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Development of func...
Development of functionalized SYBR green II related cyanine dyes for viral RNA detection
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Saarnio, Ville K. (författare)
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Salorinne, Kirsi (författare)
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- Ruokolainen, Visa P. (författare)
- Jyväskylän Yliopisto,University of Jyväskylä
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- Nilsson, Jesper, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Tero, Tiia Riikka (författare)
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- Oikarinen, Sami (författare)
- Tampereen Yliopisto,University of Tampere
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- Wilhelmsson, Marcus, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Lahtinen, Tanja M. (författare)
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- Marjomäki, Varpu S. (författare)
- Jyväskylän Yliopisto,University of Jyväskylä
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Dyes and Pigments. - : Elsevier BV. - 0143-7208 .- 1873-3743. ; 177
- Relaterad länk:
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements of RNA, are unavailable. Herein we developed cyanine dyes that can be conjugated to viral capsid or other targets. First, we solved the crystal structure of SYBR Green II. The structural elucidation of this commonly used RNA probe provided the basis for synthesizing similar molecules with much desired function for post-synthetic conjugation. To address this need, cyanine dyes were prepared using an alternative synthesis protocol. All studied compounds showed considerable brightness upon binding to nucleic acids. However, regardless of the common chromophore on the dyes, the observed fluorescence emission intensities varied significantly, where methyl-substituted dye 1 gave values higher than SYBR Green II, whereas compounds 2–5 containing undecyl spacers had lower values. Studying the structure-activity relationship revealed the longer alkyl chains to induce slight perturbation in dye intercalation, as well as demand larger binding area on the nucleic acid lattice, explaining these differences. To study the potential biological use of the dyes, the RNA genome of enterovirus echovirus 1 was studied in vitro with the probes. A novel method employing the low binding space requirement of 1 was developed to determine the single-to-double-stranded RNA ratio of a sample, whereas compound 4 was covalently bound to the viral capsid and used successfully to monitor the viral RNA release from within the capsid. The presented results open new possibilities for preparation and use of SYBR Green-based nucleic acid probes to further apply these compounds for increasingly demanding targeting in biological contexts.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Host-guest systems
- Viruses
- Cyanines
- Fluorescent probes
- RNA recognition
- Nucleic acids
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Saarnio, Ville K ...
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Salorinne, Kirsi
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Ruokolainen, Vis ...
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Nilsson, Jesper, ...
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Tero, Tiia Riikk ...
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Oikarinen, Sami
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Wilhelmsson, Mar ...
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Lahtinen, Tanja ...
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Marjomäki, Varpu ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Strukturbiologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Organisk kemi
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Dyes and Pigment ...
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Chalmers tekniska högskola