| 1. |
- Nilsson, Anders, et al.
(författare)
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Detection of Yersinia enterocolitica in food by PCR amplification
- 1998
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Ingår i: Letters in Applied Microbiology. - Oxon, United Kingdom : Blackwell Publishing. - 0266-8254 .- 1472-765X. ; 26, s. 140-144
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Tidskriftsartikel (refereegranskat)abstract
- A polymerase chain reaction (PCR) assay was developed for detection ofpathogenic, virulent strains of Yersinia enterocolitica. By using both virulence loci virFand ail as markers for pathogenicity, detection of species with a virulence factor present waspossible. DNA preparation in the presence of hexadecyl trimethy ammonium bromide(CTAB) was followed by two 44 cycle amplification reactions, one for each of themarkers. As few as 102Y. enterocolitica cells were detected in ground pork in thepresence of 105–106bacteria of other species. The described PCR assay providesa sensitive robust assay for the detection of virulent Y. enterocolitica in food.
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| 2. |
- Thisted Lambertz, Susanne, et al.
(författare)
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A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food
- 1996
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Ingår i: Journal of Applied Bacteriology. - : Blackwell Publishing. - 0021-8847. ; 81:3, s. 303-308
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to develop a polymerase chain reaction (PCR) method for the detection of pathogenic Yersinia enterocolitica and to compare it with an official culture method (NMKL-117). Primers were selected for nested PCR directed at the attachment invasion locus, ail, on the bacterial chromosome, as well as at a sequence on the pathogenic marker plasmid, termed virulence factor, virF. The final results obtained by the two methods were similar. However, while the conventional method yielded contradictory data for some steps the PCR method provided unambiguous results. Considerable advantages, i.e. higher sensitivity and specificity of the PCR method, compared with the conventional method for detecting pathogenic Y. enterocolitica, were demonstrated in this study.
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