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- Axelsson Landberg, Tove, et al.
(author)
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Hälsoprojekt ökar barns lust att röra på sig
- 2005
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In: Svensk Idrottsforskning. ; 4, s. 1-6
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Journal article (other academic/artistic)abstract
- Många skolor driver glädjande nog hälsoprojekt idag, bland annat som följd av Handslaget. Få har dock utvärderats ännu. Påverkas barnens attityder och vanor? Ett nyligen avslutat hälsoprojekt i Partille kommun tyder på detta. Det Bunkeflo-inspirerande hälsoprojektet löpte under en tvåårsperiod, ht 2002 till vt 2004, och innefattade utökad fysisk aktivitet i olika former samt massage och avslappning för de minsta barnen. Projektet följdes upp med enkäter till barnen före och efter projektet samt en fokusgruppintervju med nyckelpersonal. Resultatet har redovisats i form av en C-uppsats vid Göteborgs universitet, vilket sammanfattas i nedanstående artikel.
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| 2. |
- Landberg, Eva, 1966-, et al.
(author)
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Detection of molecular variants of prolactin in human serum, evaluation of a method based on ultrafiltration
- 2007
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In: Clinica Chimica Acta. - : Elsevier BV. - 0009-8981 .- 1873-3492. ; 376:1-2, s. 220-225
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Journal article (peer-reviewed)abstract
- BackgroundIn human blood, there are several molecular variants of prolactin with different biological effects. There is a need for new methods to detect and quantify these variants in order to fully understand the pathophysiological role of prolactin.MethodsA method based on ultrafiltration was optimized, validated and compared to PEG precipitation. Serum samples from 84 patients were analyzed before and after pre treatment on two immunoassays, Elecsys (Roche) and Access (Beckman). Protein G precipitation was used to confirm presence of macroprolactin.ResultsThe recovery of prolactin after ultrafiltration was lower than after PEG precipitation. A limit of 40% recovery after PEG precipitation corresponded to 27% recovery after ultrafiltration. Using these limits there were total agreement regarding detection of macroprolactin (rs = 0.96). In contrast, recovery of prolactin in samples without macroprolactin showed a considerable disagreement between ultrafiltration and PEG precipitation (rs = 0.48). Within-run CV was 4% for the ultrafiltration method. The correlation coefficient (r) between the immunoassays was 0.96 after ultrafiltration.ConclusionsUltrafiltration can be used to compare different prolactin immunoassays and to detect macroprolactin in assays with interference from PEG. For samples without macroprolactin ultrafiltration may give additional information reflecting individual variations of other molecular variants of prolactin.
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