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| 3. |
- Larsson, Inger, et al.
(författare)
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Interferon production in glia and glioma cell lines
- 1978
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Ingår i: Infection and Immunity. - 1098-5522. ; 22:3, s. 786-789
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Tidskriftsartikel (refereegranskat)abstract
- Interferon (IF) was produced in glia and glioma cell lines in titers comparable to those produced by human fibroblasts. It was inducible by both Sendai virus and polyriboinosinic:polyribocytidylic acid. "Superinduction" resulted in up to 500-fold-higher titers of IF. The IF appeared to be of the fibroblast type, as revealed by experiments using heat treatment, assay of antiviral activity in heterologous cell lines, and neutralization with specific antisera. Since large amounts of IF may easily be produced with glioma cell lines, such cells may be suitable for mass production of IF.
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| 4. |
- Lundgren, E, et al.
(författare)
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Effects of leukocyte and fibroblast interferon on events in the fibroblast cell cycle
- 1979
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Ingår i: Journal of General Virology. - : Microbiology Society. - 1465-2099 .- 0022-1317. ; 42:3, s. 589-595
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Tidskriftsartikel (refereegranskat)abstract
- Serum-depleted human foetal skin fibroblasts were stimulated by addition of 10% foetal calf serum to proliferate synchronously for at least one cell cycle. This proliferation was suppressed by leukocyte or fibroblast interferon (IF), which prolonged the G1 phase and diminished the rate of DNA synthesis during the S phase in a dose-dependent manner. When used in identical concentration, as judged in terms of units of antiviral activity, fibroblast IF had more pronounced effects on cell cycle events than leukocyte IF. Interferon exerted its effect in early G1, before the cells were irreversibly committed to DNA synthesis.
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| 5. |
- Miörner, Håkan, et al.
(författare)
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Regulation of mitogen-induced lymphocyte DNA synthesis by human interferon of different origins
- 1978
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 35:1, s. 15-24
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Tidskriftsartikel (refereegranskat)abstract
- Human fibroblast and leukocyte interferons were found to suppress lymphocyte mitogenesis induced by optimal doses of phytohemagglutinin and concanavalin A. In certain situations (low doses of mitogen and/or low doses of interferon), however, interferon significantly enhanced mitogenesis. In experiments using varying concentrations of interferon, dose-response curves with different slopes were obtained for fibroblast and leukocyte interferons. The effect of interferon was apparently exerted during early stages of the lymphocyte cell cycle. There was no inhibitory effect of interferon if the lymphocytes were washed with medium before being exposed to mitogen. Interferon increased the binding of radiolabeled mitogens to cells. The results suggest that the immunological effects of interferon are consequences of actions on lymphoid cells. Fibroblast and leukocyte interferons seem to have different modes of action, or to bind differently to target cells. Possible mechanisms for the suppressive and enhancing effects of interferons on lymphoid cells are discussed.
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| 6. |
- Strannegård, Örjan, et al.
(författare)
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Modulation of immune responses in newborn and adult mice by interferon
- 1978
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Ingår i: Infection and Immunity. - 1098-5522. ; 20:2, s. 334-339
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Tidskriftsartikel (refereegranskat)abstract
- Interferon was found to have both suppressive and enhancing effects on the antibody response in newborn and adult mice. Evidence was obtained that these effects are primarily evoked during the initial steps controlling cell proliferation. Stimulation of thymus and spleen cells with a T-cell mitogen was enhanced by low doses and suppressed by high doses of interferon. Treatment of parental spleen cells with interferon before injecting them into immunized F1 hybrid mice resulted in an enhanced allogeneic effect. These results are compatible with the hypothesis that interferon affects T cells and has an immunoregulatory role, either by inhibiting the action of suppressor cells or by promoting immunological maturation.
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