| 1. |
- Basu, Swaraj, et al.
(författare)
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Accurate mapping of mitochondrial DNA deletions and duplications using deep sequencing
- 2020
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Ingår i: PLoS Genetics. - : Public Library of Science (PLoS). - 1553-7404. ; 16:12
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Tidskriftsartikel (refereegranskat)abstract
- Deletions and duplications in mitochondrial DNA (mtDNA) cause mitochondrial disease and accumulate in conditions such as cancer and age-related disorders, but validated high-throughput methodology that can readily detect and discriminate between these two types of events is lacking. Here we establish a computational method, MitoSAlt, for accurate identification, quantification and visualization of mtDNA deletions and duplications from genomic sequencing data. Our method was tested on simulated sequencing reads and human patient samples with single deletions and duplications to verify its accuracy. Application to mouse models of mtDNA maintenance disease demonstrated the ability to detect deletions and duplications even at low levels of heteroplasmy. Author summary Deletions in the mitochondrial genome cause a wide variety of rare disorders, but are also linked to more common conditions such as neurodegeneration, diabetes type 2, and the normal ageing process. There is also a growing awareness that mtDNA duplications, which are also relevant for human disease, may be more common than previously thought. Despite their clinical importance, our current knowledge about the abundance, characteristics and diversity of mtDNA deletions and duplications is fragmented, and based to large extent on a limited view provided by traditional low-throughput analyses. Here, we describe a bioinformatics method, MitoSAlt, that can accurately map and classify mtDNA deletions and duplications using high-throughput sequencing. Application of this methodology to mouse models of mitochondrial deficiencies revealed a large number of duplications, suggesting that these may previously have been underestimated.
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| 2. |
- Larsson, Ingrid, 1968-, et al.
(författare)
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Patient Empowerment and Associations with Disease Activity and Pain‐Related and Lifestyle Factors in Patients With Rheumatoid Arthritis
- 2021
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Ingår i: ACR Open Rheumatology. - Hoboken, NJ : John Wiley & Sons. - 2578-5745. ; 3:12, s. 842-849
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Tidskriftsartikel (refereegranskat)abstract
- Background: Empowerment is important to patients with rheumatoid arthritis (RA) because most care is in the form of self-management. The aim was to study levels of empowerment and associated variables in patients with RA and to investigate longitudinal clinical data in patients with low and high empowerment.Methods: A postal survey was sent in 2017 to patients with RA from the BARFOT (Better Anti-Rheumatic Pharmacotherapy) cohort that included questions about disease activity, pain-related factors, lifestyle habits, and contained the Swedish Rheumatic Disease Empowerment Scale (SWE-RES-23). The 844 patients who answered the SWE-RES-23 made up the cohort of the present study. Differences in level of empowerment between groups (low, moderate, and high empowerment) were analyzed with ANOVA. Logistic regression analysis was used to study variables associated with low empowerment. Thirdly, we performed comparisons in longitudinal data (15 years) of disease activity, pain, and physical function between the three empowerment groups (low, moderate, and high empowerment).Results: Patients with low empowerment (n = 282) were significantly older, more often women, and reported worse pain-related factors and physical function and lower moderate and vigorous physical activity compared with those with high empowerment (n = 270). An analysis of longitudinal data found that patients with low empowerment had worse pain and physical function at all time points.Conclusion: Patients with low empowerment have more pain-related symptoms, poorer physical function, and are less physically active. To promote patient empowerment in rehabilitation interventions it is important to identify and support self-management. © 2021 The Authors.
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| 3. |
- Sorgenfrei, Simon, Docent, et al.
(författare)
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Mångreligiositet och sekularitet i svenskt polisväsende, vård, skola och offentlig förvaltning : en forskningsöversikt
- 2021
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Under de senaste decennierna har Sverige genomgått stora demografiska och politiska förändringar. Tillsammans har dessa inneburit att Sverige idag samtidigt är ett av Europas mest sekulariserade och mest mångreligiösa länder. Den snabba demografiska förändring Sverige har genomgått har ställt många inför nya, stora och i vissa fall skyndsamma kunskapsbehov samtidigt är forskningen om situationen delvis eftersatt. I denna rapport identifieras hur den nya situationen relaterar till det lagstadgade uppdrag som svenskt polisväsende, vård, skola och offentlig förvaltning har. Den forskning som gjorts inom dessa områden sammanfattas och de viktigaste forskningsbehoven identifieras.
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| 4. |
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| 5. |
- Sunesson, Evelina, et al.
(författare)
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Experiences of How Health and Lifestyle among Individuals with Knee Pain Have Been Influenced during the COVID-19 Pandemic, a HALLOA Study
- 2022
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Ingår i: International Journal of Environmental Research and Public Health. - Basel : MDPI. - 1661-7827 .- 1660-4601. ; 19:14
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Tidskriftsartikel (refereegranskat)abstract
- The COVID-19 pandemic has affected the health and lifestyles of both the general population and of vulnerable groups. Individuals with knee pain are recommended to lead an active lifestyle to relieve pain but find it difficult to maintain health and lifestyle compared to the general population due to the cause of chronic pain, impaired physical function, and a diminished quality of life. This study aimed to explore experiences of how health and lifestyle among individuals with knee pain have been influenced during the COVID-19 pandemic. Interviews (n = 19) were conducted in 2021 and analysed with qualitative content analysis. The results showed how individuals with knee pain adjusted their behaviour and revalued their life to maintain health and lifestyle during COVID-19. Adjusted behaviours emerged, such as spending more time at home, becoming digital, and spending more time outdoors, while revaluing life emerged as having a positive outlook on life and sharing responsibility. In conclusion, behaviour was adjusted, and life revalued to manage health and lifestyle during the pandemic. However, the findings are probably similar to the general population, i.e., individuals with knee pain live similar lives as the general population despite knee pain. The results may contribute to alternative ways of maintaining health and lifestyle in various vulnerable groups and may be applied in situations other than the pandemic. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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| 6. |
- Sunesson, Evelina, et al.
(författare)
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Experiences of how health and lifestyle among individuals with knee pain have been influenced during the Covid-19 pandemic, a halloa study
- 2022
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background: The covid-19 pandemic has affected health and lifestyle among the general population and vulnerable groups. Individuals with knee pain are recommended an active lifestyle to reveal pain but find it difficult to maintain health and lifestyle compared to the general population due to the cause of chronic pain, impaired physical function, and a diminished quality of life. This adds to the importance of exploring how to preserve health and lifestyle among individuals with knee pain during the pandemic. Objective: The aim of this study was to explore experiences of how health and lifestyle among individuals with knee pain have been influenced during the covid-19 pandemic.Method: Nineteen participants with current knee pain, with no earlier diagnosed radiographic osteoarthritis, and no rheumatologic disorder or cruciate ligament injury, were recruited from The Halland Osteoarthritis (HALLOA) Cohort Study, twelve females and seven males, between 41-62 (median 51) years of age. Data were collected through individual interviews with open-ended questions: “What impact have you experienced with your health and lifestyle during the covid-19 pandemic?”, and “What activities or strategies have you changed to maintain your health and lifestyle during the covid-19 pandemic? ”. Qualitative content analysis was used, where two categories and five sub-categories emerged (Table 1). Result: The result from this study explored how individuals with knee pain adjusted their behaviour and revalued their life to maintain health and lifestyle during the pandemic. The category adjusted behaviours emerged with the sub-categories: spending more time at home, becoming digitally and spending more time outdoors. These sub-categories determine how a more reclusive behaviour has appeared due to the pandemic, where digital platforms and outdoor activities have facilitated companionship and togetherness when feelings of loneliness and isolation were present. The category revaluing life emerged with the sub-categories: having a positive outlook on life and sharing responsibility. These sub-categories establish the importance of being grateful for maintaining health and lifestyle and being solution-oriented to find the best possible outcomes to continue with everyday life, despite the pandemic. While having a responsibility toward others arose as a facility to manage to keep social distance during the pandemic. Conclusion: In conclusion, individuals with knee pain managed their health and lifestyle during the pandemic by adjusting behaviors and revaluing life. Adapting to restrictions included spending more time at home and outdoors, becoming more digitally, and revaluing life by having a positive outlook on life and supporting others. The result may contribute to alternative ways of preserving health and lifestyle in different vulnerable groups and may be used in situations other than the pandemic.
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| 7. |
- Sunesson, Evelina, et al.
(författare)
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The Impact of the Covid-19 pandemic on Health and Lifestyle in Individuals with Knee Pain : a HALLOA study
- 2022
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Konferensbidrag (refereegranskat)abstract
- Background: Covid-19 has affected everyday life, health and lifestyle among the general population and vulnerable groups. Individuals with knee pain are recommended an active lifestyle to reveal pain but find it difficult to maintain health and lifestyle compared to the general population due to the cause of chronic pain, impaired physical function and a diminishes quality of life. This adds to the importance of exploring how to preserve health and lifestyle among individuals with knee pain during the pandemic.Objectives: The aim of this study was to explore the impact of the covid-19 pandemic on the health and lifestyle in individuals with knee pain.Methods: Nineteen participants with current knee pain, and with no earlier diagnosed radiographic osteoarthritis, and no rheumatologic disorder or cruciate ligament injury, were recruited from The Halland Osteoarthritis (HALLOA) Cohort Study, twelve female and seven males, between 41-62 (median 51) years of age. Data were collected through individual interviews with open-ended questions: “What impact have you experienced with your health and lifestyle during covid-19?”, and “What activities or strategies have you changed to maintain your health and lifestyle during covid-19? ”. Qualitative content analysis was used, where two categories and five sub-categories emerged ( Table 1 ).Results: The result from this study explored how behaviour and attitude towards valuing life have been adjusted to maintain health and lifestyle among individuals with knee pain during covid-19. The category adjusted behaviours emerged with the sub-categories: spending more time at home, becoming digitally, and spending more time outdoors. These sub-categories determine how a more reclusive behaviour have appeared due to the pandemic, where digital platforms and outdoor activities have facilitated companionship and togetherness when feelings of loneliness and isolation were present. The category valuing life emerged with the sub-categories: having a positive outlook of life and sharing responsibility. These sub-categories establish the importance of trying to be grateful for maintaining health and lifestyle and trying to be solution-oriented to find the best possible outcomes to continue with everyday life, despite the pandemic. While having a responsibility towards others arose as a facility to manage to keep social distance during the pandemic.Conclusion: In conclusion, the behaviour was adjusted to continue with everyday life and maintain health and lifestyle among individuals with knee pain during covid-19. Valuing life was also considered important to maintain health and lifestyle as well as supporting others maintain their health and lifestyle. The result may in the future contribute to alternative ways of maintaining health and lifestyle in different vulnerable groups and may be used in situations other than the pandemic.
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| 8. |
- Sylwander, Charlotte, 1992-, et al.
(författare)
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Experiences of Health-Promoting Activities among Individuals with Knee Pain : The Halland Osteoarthritis Cohort
- 2022
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Ingår i: International Journal of Environmental Research and Public Health. - Basel : MDPI. - 1661-7827 .- 1660-4601. ; 19:17
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Tidskriftsartikel (refereegranskat)abstract
- Knee pain is an early sign of knee osteoarthritis (KOA) and a risk factor for chronic widespread pain (CWP). Early prevention is vital, and more research is needed to understand health-promoting activities for individuals with knee pain from a patient perspective. This study aimed to explore how individuals with knee pain experienced health-promoting activities. Explorative qualitative design with inductive approach was applied to explore the experiences of 22 individuals (13 women, 9 men; median age: 52). Semi-structured interviews were conducted and analysed using latent qualitative content analysis. The results revealed health-promoting activities in individuals with knee pain and were interpreted in the overall theme, striving for balance in everyday life. Two categories explored the content in health-promoting activities: (1) Caring for the body—being physically active, having a healthy diet, and utilising support; and (2) Managing life stressors—allowing for recovery, promoting vitality, and safeguarding healthy relationships. In conclusion, individuals with knee pain described various health-promoting activities. They strived for balance in everyday life by caring for the body and managing life stressors. We suggest that a broader approach to everyday life can be helpful in treatment plans and health promotion to manage and prevent KOA and CWP, while striving for a healthy lifestyle.
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| 9. |
- Sylwander, Charlotte, 1992-, et al.
(författare)
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Health literacy in individuals with knee pain-a mixed methods study
- 2023
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Ingår i: BMC Public Health. - London : BioMed Central (BMC). - 1471-2458. ; 23, s. 1-16
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Low health literacy is associated with worse pain and poorer self-management. This study (1) examined the level of health literacy and associations with lifestyle habits, health status, chronic pain, and radiographic knee osteoarthritis; and (2) explored experiences illuminating health literacy among individuals with knee pain.METHODS: A convergent parallel mixed-methods design was used, including 221 individuals. Health literacy was assessed by HLS-EU-Q16 and eHEALS. The questionnaire included questions on lifestyle habits, health status, and pain distribution. Radiographic knee osteoarthritis was assessed with x-rays. Associations were analysed using logistic regression analyses. Individual semi-structured interviews were conducted (n = 19) and analysed with qualitative content analysis.RESULTS: The result showed that 71% reported sufficient health literacy. Higher education, healthy lifestyle habits, better general health, and absence of widespread pain were associated with sufficient health literacy. Experiences regarding health literacy influencing the decision-making process toward a decision on action comprised: (1) searching for information actively or passively; (2) processing of the information included being informed, critical, and interpretive; and (3) taking a stand on the information based on trustfulness and motivation.CONCLUSION: Seven out of 10 reported sufficient health literacy. Despite this, unhealthy lifestyles were common, suggesting that having sufficient HL is not enough for a behavioural change and the decision-making process, including different phases such as searching, processing, and taking a stand on health information is important to consider. More research on health literacy is needed to gain knowledge of how best to develop health promotion in individuals with knee pain. © 2023, BioMed Central Ltd., part of Springer Nature.
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| 10. |
- Sylwander, Charlotte, 1992-, et al.
(författare)
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Health Promotion in Individuals with Knee Pain – Different Ways to Enable and Maintain a Balance in Everyday Life Based on the Abilities
- 2022
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background: Most individuals with knee pain develop radiographic knee osteoarthritis over time [1], and up to 30% develop chronic widespread pain (CWP) [2]. Many studies only focus on physical activity or weight loss when it comes to managing knee osteoarthritis (KOA) and chronic pain. There is a challenge to get more individuals to follow the existing recommendations for KOA, including physical activity and weight loss [3]. However, the interaction between the various physical, mental, and social factors regarding health promotion is complex. More research is needed to understand health-promoting activities applied amongst individuals with knee pain.Objectives: To explore health-promoting activities in individuals with knee pain.Methods: The study was a part of the Halland Osteoarthritis cohort (ClinicalTrials.gov NCT04928170), including 306 individuals with knee pain without earlier diagnosed KOA, cruciate ligament injury or other rheumatological disorder. A purposeful sample of 22 individuals (13 women, 9 men; median age 52 years) participated in the study. Ten had radiographic KOA, 12 had chronic regional pain, 9 reported CWP, 16 were overweight, and 13 met the WHO recommendations for physical activity with moderate intensity. Semi-structured interviews were conducted and followed a pilot-tested interview guide initiated with open-ended questions such as “What do you do to maintain your health?” and “What enables you to keep health?”. The interviewers followed the participants reasoning, and to obtain depth in the data, the participant received follow-up questions such as “Please, can you tell me more about…?”. The interviews were audio-recorded and transcribed verbatim. Data analysis was performed by latent qualitative content analysis and revealed an overall theme including two categories and six sub-categories.Results: Health-promoting activities in individuals with knee pain were interpreted in the overall theme; enabling and maintaining a balance in everyday life based on abilities. Two categories explored the content in health-promoting activities as 1) Using facilitators to take care of the body with the sub-categories: being physically active, having a healthy diet, and using external resources. For example, the participants took care of the body by being physically active to decrease pain, reducing sugar intake and using external resources such as online workouts or help from health care. 2) Managing stressors of everyday life with the sub-categories: promoting vitality, allowing for recovery, and safeguarding healthy relationships. The participants managed stressors of life by, for example, being in nature, planning their work- and private life and spending time with friends and family. See Table 1 Conclusion: Individuals with knee pain described various health-promoting activities, ranging from physical activity to social interactions, included in the theme of enabling and maintaining a balance in everyday life based on abilities. We suggest that a broader approach to everyday life can be helpful in treatment plans and health promotion to manage and prevent KOA and chronic pain while maintaining a healthy lifestyle.REFERENCES:[1] Thorstensson et al. Natural course of knee osteoarthritis in middle-aged subjects with knee pain… . ARD, 2009;68:1890-1893.[2] Bergman et al. Chronic widespread pain and its associations with quality of life and function at a 20-year follow-up… . BMC Musculoskel, 2019;20:592[3] Fernandes et al. EULAR recommendations for the non-pharmacological core management of hip and knee osteoarthritis . ARD, 2013;72:1128
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