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Träfflista för sökning "WFRF:(Lasser Theo) srt2:(2005-2009)"

Sökning: WFRF:(Lasser Theo) > (2005-2009)

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1.
  • Hassler, Kai, et al. (författare)
  • Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy
  • 2007
  • Ingår i: Optics Express. - 1094-4087. ; 15:9, s. 5366-5375
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.
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2.
  • Leutenegger, Marcel, et al. (författare)
  • Dual-color total internal reflection fluorescence cross-correlation spectroscopy
  • 2006
  • Ingår i: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 11:4
  • Tidskriftsartikel (refereegranskat)abstract
    • We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation spectroscopy (FCCS). As a performance analysis, we measured a synthetic DNA-binding assay, demonstrating this dual-color TIR-FCCS approach to be a suitable method for measuring coincidence assays such as biochemical binding, fusion, or signal transduction at solid/liquid interfaces. Due to the very high numerical aperture of the epi-illumination configuration, our setup provides a very high fluorescence collection efficiency resulting in a two- to three- fold increase in molecular brightness compared to conventional confocal FCCS. Further improvements have been achieved through global analysis of the spectroscopic data.
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