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Träfflista för sökning "WFRF:(Lee S. H) srt2:(1990-1994)"

Sökning: WFRF:(Lee S. H) > (1990-1994)

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1.
  • Szymanski, J. J., et al. (författare)
  • MEGA : A search for the decay mu –> e gamma
  • 1994
  • Ingår i: Intersections between particle and nuclear physics. Proceedings, 5th Conference, St. Petersburg, USA, May 31-June 6, 1994. ; , s. 789-792
  • Konferensbidrag (refereegranskat)
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2.
  • Birnir, Bryndis, et al. (författare)
  • Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells.
  • 1990
  • Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1048:1, s. 100-4
  • Tidskriftsartikel (refereegranskat)abstract
    • Cells derived from the simian kidney, COS-7 cells, were transfected with a eucaryotic expression vector (pEUK-C1) containing the clone for the rabbit intestinal Na+/glucose cotransporter. Expression was monitored after transfection with lipofectin by measuring the initial rate of alpha-methylglucopyranoside (MeGlc) uptake. Cells transfected with vector containing the cDNA for the Na+/glucose cotransporter expressed Na(+)-dependent MeGlc transport. Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. Na(+)-dependent MeGlc uptake into transfected cells was saturable (Km 150 microM), phlorizin-sensitive (Ki 11 microM), and inhibited by sugar analogs (D-glucose greater than MeGlc greater than D-galactose greater than 3-O-methyl-D-glucoside greater than D-allose much greater than L-glucose). Europium was able to mimic Na+ in driving MeGIC uptake. Finally, tunicamycin, an inhibitor of asparagine-linked glycosylation, inhibited the expression of Na(+)-dependent MeGlc transport 80%. We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic properties to that in the native brush border and to that expressed in Xenopus oocytes. In addition, N-linked glycosylation appears to be important for functional expression of this membrane protein.
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