| 1. |
- Hilson, P., et al.
(author)
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Versatile gene-specific sequence tags for Arabidopsis functional genomics : Trancript profiling and reverse genetics applications
- 2004
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In: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 14:10B, s. 2176-2189
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Journal article (peer-reviewed)abstract
- Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
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| 2. |
- Castillo Leon, Jaime, et al.
(author)
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Biosensors for life quality - Design, development and applications
- 2004
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In: Sensors and Actuators B: Chemical. - : Elsevier BV. - 0925-4005. ; 102:2, s. 179-194
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Journal article (peer-reviewed)abstract
- Biosensors, combining a selective biological recognition element and a sensitive transducer, are versatile analytical tools applied more and more in different fields, such as medicine, food quality and safety control, and environment pollution monitoring. They are expected to play an increasingly important role in the improvement of life quality. In this context, the present work covers recent approaches in design and development of biosensors applied for analysis of real samples of medical, environmental or industrial relevance. The described sensors meet the sensitivity, selectivity, and response time required by their applications. Moreover, they are designed to avoid contamination of the monitored systems with undesirable components and to minimise the damage of living organisms (when it comes to in vivo monitoring). (C) 2004 Elsevier B.V. All rights reserved.
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| 3. |
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| 4. |
- Winblad, B, et al.
(author)
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Mild cognitive impairment--beyond controversies, towards a consensus: report of the International Working Group on Mild Cognitive Impairment.
- 2004
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In: Journal of internal medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 256:3, s. 240-6
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Research review (peer-reviewed)abstract
- The First Key Symposium was held in Stockholm, Sweden, 2-5 September 2003. The aim of the symposium was to integrate clinical and epidemiological perspectives on the topic of Mild Cognitive Impairment (MCI). A multidisciplinary, international group of experts discussed the current status and future directions of MCI, with regard to clinical presentation, cognitive and functional assessment, and the role of neuroimaging, biomarkers and genetics. Agreement on new perspectives, as well as recommendations for management and future research were discussed by the international working group. The specific recommendations for the general MCI criteria include the following: (i) the person is neither normal nor demented; (ii) there is evidence of cognitive deterioration shown by either objectively measured decline over time and/or subjective report of decline by self and/or informant in conjunction with objective cognitive deficits; and (iii) activities of daily living are preserved and complex instrumental functions are either intact or minimally impaired.
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