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Träfflista för sökning "WFRF:(Li B) srt2:(1995-1999)"

Sökning: WFRF:(Li B) > (1995-1999)

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  • Jansson, Magnus, et al. (författare)
  • High-level production of uniformly 15N- and 13C-enriched fusion proteins in Escherichia coli.
  • 1996
  • Ingår i: Journal of Biomolecular NMR. - 0925-2738 .- 1573-5001. ; 7:2, s. 131-41
  • Tidskriftsartikel (refereegranskat)abstract
    • An approach to produce 13C- and 15N-enriched proteins is described. The concept is based on intracellular production of the recombinant proteins in Escherichia coli as fusions to an IgG-binding domain, Z, derived from staphylococcal protein A. The production method provides yields of 40-200 mg/l of isotope-enriched fusion proteins in defined minimal media. In addition, the Z fusion partner facilitates the first purification step by IgG affinity chromatography. The production system is applied to isotope enrichment of human insulin-like growth factor II (IGF-II), bovine pancreatic trypsin inhibitor (BPTI), and Z itself. High levels of protein production are achieved in shaker flasks using totally defined minimal medium supplemented with 13C(6)-glucose and (15NH4)2SO4 as the only carbon and nitrogen sources. Growth conditions were optimized to obtain high protein production levels and high levels of isotope incorporation, while minimizing 13C(6)-glucose usage. Incorporation levels of 13C and/or 15N isotopes in purifies IGF-II, BPTI, and Z were confirmed using mass spectrometry and NMR spectroscopy. More than 99% of total isotope enrichment was obtained using a defined isotope-enriched minimal medium. The optimized systems provide reliable, high-level production of isotope-enriched fusion proteins. They can be used to produce 20-40 mg/l of properly folded Z and BPTI proteins. The production system of recombinant BPTI is state-of-the-art and provides the highest known yield of native refolded BPTI.
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  • Lee, SY, et al. (författare)
  • Effect of magnetized electron cooling on a Hopf bifurcation
  • 1996
  • Ingår i: PHYSICAL REVIEW E. - : AMER INST PHYSICS. ; 53:1, s. 1287-1290
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • We have observed longitudinal limit cycle oscillations of a proton beam when a critical threshold in the relative velocity between the proton beam and the cooling electrons has been exceeded. The threshold for the bifurcation of a fixed point into a limit
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  • Li, W.B., et al. (författare)
  • A kinetic model for reaction bonding process of silicon powder compact
  • 1997
  • Ingår i: Journal of the European Ceramic Society. - 0955-2219 .- 1873-619X. ; 17:9, s. 1119-1129
  • Tidskriftsartikel (refereegranskat)abstract
    • In order to obtain detailed information about the kinetics and the reaction nature of a complex reaction process like reaction bonding of silicon nitride, mathematical modelling of the process is necessary. The previous quantitative models for this process have been based only on the mechanism that the nitrogen diffuses through the solid silicon nitride without taking into account the multiple reaction mechanisms. In the present study, a comprehensive kinetic model, which is based on analysis of the multiple mechanisms in a silicon powder compact reacting with nitrogen gas and forming silicon nitride, is constructed for a solid-gas reaction bonding process with specific application to the reaction-bonding of silicon nitride. The model will incorporate the rate equation for each mechanism into a constitutive equation from which more complete information of process kinetics can be predicted. The results predicted by the present model have been compared with previous experimental results and satisfactory agreement obtained
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