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A novel radioligand...
A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM
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- Grubin, C. E. (författare)
- University of Washington
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- Daniels, T. (författare)
- University of Washington
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- Toivola, B. (författare)
- University of Washington
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- Landin-Olsson, M. (författare)
- Lund University,Lunds universitet,Medicin/akutsjukvård, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Diabetes lab,Forskargrupper vid Lunds universitet,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups
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- Hagopian, W. A. (författare)
- University of Washington
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- Li, L. (författare)
- University of Washington
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- Karlsen, A. E. (författare)
- Hagedorn Research Institute
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- Boel, E. (författare)
- Novo Nordisk A/S
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- Michelsen, B. (författare)
- Hagedorn Research Institute
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- Lernmark, Å (författare)
- Karolinska Institute,Karolinska University Hospital,University of Washington
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Diabetologia. - 0012-186X. ; 37:4, s. 344-350
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Insulin-dependent diabetes mellitus (IDDM) is associated with autoreactivity against GAD but the diagnostic sensitivity (positivity in disease) and specificity (negativity in health) of isoform-specific GAD antibodies have yet to be defined in assay systems suitable for screening large number of samples. One set of IDDM patient (n=10) and control (n=50) standard sera were used to develop quantitative antibody assays with in vitro synthesized recombinant 35S-methionine-labelled GAD65 and GAD67, respectively, and protein A-Sepharose to separate free from antibody-bound ligand. Binding levels were not normally distributed (p<0.0001) and therefore, the diagnostic accuracy of GAD antibodies was analysed by the ROC plots in population-based, consecutively-diagnosed, recent onset, 0-14 year-old patients (n=105), and matched, healthy control subjects (n=157). The ROC plots showed that the diagnostic sensitivity of GAD65 antibodies was 77% and the specificity 92% compared with 8% and 98%, respectively for GAD67 antibodies. In the IDDM sera, GAD65 and GAD67 antibodies were concordant in 7% (6 of 81) and GAD65 antibodies and ICA in 89% (72 of 81) without a correlation between the autoantibody levels. Autoantibodies to recombinant human islet GAD65 are specific and sensitive markers for childhood IDDM in this immunoassay with in vitro synthesized 35S-methioninelabelled recombinant GAD.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- autoimmunity
- diabetes mellitus
- diagnostic accuracy
- Glutamic acid decarboxylase
- islet cell antibodies
- receiver-operating characteristic plot
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Grubin, C. E.
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Daniels, T.
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Toivola, B.
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Landin-Olsson, M ...
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Hagopian, W. A.
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Li, L.
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visa fler...
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Karlsen, A. E.
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Boel, E.
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Michelsen, B.
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Lernmark, Å
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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Diabetologia
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Lunds universitet