| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Kristan, Matej, et al.
(författare)
-
The first visual object tracking segmentation VOTS2023 challenge results
- 2023
-
Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
-
Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
|
|
| 3. |
- Wang, Fang, et al.
(författare)
-
Emerging contaminants: A One Health perspective
- 2024
-
Ingår i: Innovation. - 2666-6758. ; 5
-
Forskningsöversikt (refereegranskat)abstract
- Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the continuous introduction of new substances remains a major threat to both people and the planet. In response, global initiatives are focusing on risk assessment and regulation of emerging contaminants, as demonstrated by the ongoing efforts to establish the UN's Intergovernmental Science-Policy Panel on Chemicals, Waste, and Pollution Prevention. This review identifies the sources and impacts of emerging contaminants on planetary health, emphasizing the importance of adopting a One Health approach. Strategies for monitoring and addressing these pollutants are discussed, underscoring the need for robust and socially equitable environmental policies at both regional and international levels. Urgent actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.
|
|
| 4. |
- Qi, Zhenyu, et al.
(författare)
-
Blueshifting the Absorption of a Small -Molecule Donor and Using it as the Third Component to Achieve High-Efficiency Ternary Organic Solar Cells
- 2022
-
Ingår i: Solar RRL. - : WILEY-V C H VERLAG GMBH. - 2367-198X. ; 6:9
-
Tidskriftsartikel (refereegranskat)abstract
- Adding a small-molecule donor (SMD) to state-of-the-art nonfullerene organic solar cells (OSCs) is demonstrated as a useful strategy to construct ternary organic solar cells, as SMDs typically have high crystallinity and can tune charge transport properties of OSCs. However, the absorption of most SMDs overlaps with typical donor polymers (e.g., PM6), which is against the general guidelines of adopting materials with complementary absorption in ternary OSCs. Herein, the absorption of state-of-art SMDs (BTR-CI) by linking the beta position of the outer thiophene to the alpha position of the inner thiophene unit is intentionally blueshifted. The resulting molecule beta-S1 shows a maximum absorption peak at 505 nm in the film state, which exhibits wider bandgap and shows complementary absorption with the host system (PM6:Y6). The corresponding ternary OSCs with 20%wt beta-S1 show significantly enhanced efficiency from 16.2% to 17.1% due to the increased short-circuit current (J(sc)) and improved fill factor (FF). Herein, an effective strategy to design SMDs with both wider bandgaps and higher crystallinity for high-performance ternary OSCs is presented.
|
|
| 5. |
|
|
| 6. |
- Li, Han, et al.
(författare)
-
Advancements of nature nanocage protein : preparation, identification and multiple applications of ferritins
- 2022
-
Ingår i: Critical reviews in food science and nutrition. - : Informa UK Limited. - 1040-8398 .- 1549-7852. ; 62:25, s. 7117-7128
-
Forskningsöversikt (refereegranskat)abstract
- Ferritin is an important iron storage protein, which is widely existed in all forms of life. Ferritin can regulate iron homeostasis when iron ions are lacking or enriched in the body, so as to avoid iron deficiency diseases and iron poisoning. Ferritin presents a hollow nanocage, which can store ions or other small molecular substances in the cavity. Therefore, ferritin shows its potential as a functional nanomaterial that can deliver nutrients or drugs in a targeted manner to improve bioavailability. Due to the special structure, the research on ferritin has attracted more and more attention in recent years. In this paper, the structural characteristics of ferritin were introduced, and the natural purification and prokaryotic expression methods of ferritin from different sources were described. At the same time, ferritin can bind to small molecules, so that it has the activity of small molecules, to construct a new type of ferritin. As a result, ferritin plays an important role as a nutrient substance, in targeted transport, and disease monitoring, etc. In conclusion, the yield of ferritin can be improved by means of molecular biology. Meanwhile, molecular modification can be used to make ferritin have unique activity and function, which lays a foundation for subsequent research.
|
|
| 7. |
- Li, Han, et al.
(författare)
-
Improvement of thermal stability of oyster (Crassostrea gigas) ferritin by point mutation
- 2021
-
Ingår i: Food Chemistry. - : Elsevier. - 0308-8146 .- 1873-7072. ; 346
-
Tidskriftsartikel (refereegranskat)abstract
- Ferritin can be widely used as functional nanomaterial. But the physiological activity of ferritin can be damaged under excessive temperatures, which affect the self-assembly property. In this study, point mutation was produced in Asp120 to Gly120 of ferritin amino acid sequence and the heat resistance was improved significantly. The thermal denaturation temperature of mutated ferritin is 89.17 degrees C and has increased by 13 degrees C more than the wildtype oyster ferritin. The effect of thermal treatment on the denaturation, aggregation state, particle size and the structure of ferritin was not changed before 90 degrees C. The computational modeling and analysis indicated that mutated ferritin promotes the overall structural stability assembly via decreasing the interaction energies of 62 percent energies in 3-fold interface. Improving the thermal stability of oyster ferritin by point mutation enhances its applications as a food ingredient.
|
|
| 8. |
- Li, Xin, et al.
(författare)
-
Halide Double Perovskite Nanocrystals Doped with Rare-Earth Ions for Multifunctional Applications
- 2023
-
Ingår i: Advanced Science. - 2198-3844. ; 10:20
-
Tidskriftsartikel (refereegranskat)abstract
- Most lead-free halide double perovskite materials display low photoluminescence quantum yield (PLQY) due to the indirect bandgap or forbidden transition. Doping is an effective strategy to tailor the optical properties of materials. Herein, efficient blue-emitting Sb3+-doped Cs2NaInCl6 nanocrystals (NCs) are selected as host, rare-earth (RE) ions (Sm3+, Eu3+, Tb3+, and Dy3+) are incorporated into the host, and excellent PLQY of 80.1% is obtained. Femtosecond transient absorption measurement found that RE ions not only served as the activator ions but also filled the deep vacancy defects. Anti-counterfeiting, optical thermometry, and white-light-emitting diodes (WLEDs) are exhibited using these RE ions-doped halide double perovskite NCs. For the optical thermometry based on Sm3+-doped Cs2NaInCl6:Sb3+ NCs, the maximum relative sensitivity is 0.753% K−1, which is higher than those of most temperature-sensing materials. Moreover, the WLED fabricated by Sm3+-doped Cs2NaInCl6:Sb3+ NCs@PMMA displays CIE color coordinates of (0.30, 0.28), a luminous efficiency of 37.5 lm W−1, a CCT of 8035 K, and a CRI over 80, which indicate that Sm3+-doped Cs2NaInCl6:Sb3+ NCs are promising single-component white-light-emitting phosphors for next-generation lighting and display technologies.
|
|
| 9. |
- Li, Zhenyu, et al.
(författare)
-
Mixed Static and Reconfigurable Metasurface Deployment in Indoor Dense Spaces : How Much Reconfigurability is Needed?
- 2024
-
Ingår i: 2024 IEEE Wireless Communications and Networking Conference, WCNC 2024 - Proceedings. - : Institute of Electrical and Electronics Engineers (IEEE).
-
Konferensbidrag (refereegranskat)abstract
- In this paper, we investigate how metasurfaces can be deployed to deliver high data rates in a millimeter-wave (mmWave) indoor dense space with many blocking objects. These surfaces can either be static metasurfaces (SMSs) that reflect with fixed phase-shifts or reconfigurable intelligent surfaces (RISs) that can reconfigure their phase-shifts to the currently served user. The latter comes with an increased power, cabling, and signaling cost. To see how reconfigurability affects the network performance, we propose an iterative algorithm based on the feasible point pursuit successive convex approximation method. We jointly optimize the types and phase-shifts of the surfaces and the time portion allocated to each user equipment to maximize the minimum data rate achieved by the network. Our numerical results demonstrate that the minimum data rate improves as more RISs are introduced but the gain diminishes after some point. Therefore, introducing more reconfigurability is not always necessary. Another result shows that to reach the same data rate achieved by using 22 SMSs, at least 18 RISs are needed. This suggests that when it is costly to deploy many RISs, as an inexpensive alternative solution, one can reach the same data rate just by densely deploying more SMSs.
|
|
| 10. |
- Li, Zhenyu, et al.
(författare)
-
mmWave Coverage Extension Using Reconfigurable Intelligent Surfaces in Indoor Dense Spaces
- 2023
-
Ingår i: ICC 2023 - IEEE International Conference on Communications. - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 5805-5810
-
Konferensbidrag (refereegranskat)abstract
- In this work, we consider the deployment of reconfigurable intelligent surfaces (RISs) to extend the coverage of a millimeter-wave (mmWave) network in indoor dense spaces. We first integrate RIS into ray-tracing simulations to realistically capture the propagation characteristics, then formulate a non-convex optimization problem that minimizes the number of RISs under rate constraints. We propose a feasible point pursuit and successive convex approximation-based algorithm, which solves the problem by jointly selecting the RIS locations, optimizing the RIS phase-shifts, and allocating time resources to user equipments (UEs). The numerical results demonstrate substantial coverage extension by using at least four RISs, and a data rate of 130 Mbit/s is guaranteed for UEs in the considered area of an airplane cabin.
|
|
