| 1. |
- Chen, Daru, et al.
(författare)
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Single-longitudinal-mode erbium-doped fiber laser based on a fiber Bragg grating Fabry-Perot filter
- 2007
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Ingår i: Laser physics. - 1054-660X .- 1555-6611. ; 17:10, s. 1246-1248
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Tidskriftsartikel (refereegranskat)abstract
- A novel single-longitudinal-mode (SLM) erbium-doped fiber laser with a simple linear cavity based on a fiber Bragg grating Fabry-Perot filter (FBG-FPF) and a narrowband (similar to 0.06 nm) FBG is proposed and demonstrated experimentally. Two uniform FBGs form the FBG-FPF, which has two ultranarrow transmission bands with a bandwidth of 0.12 pm and a wavelength spacing of 0.095 nm. By slightly tuning the central wavelength of the narrowband FBG, SLM lasing at 1549.658 or 1549.563 nm (corresponding to the two transmission peaks of the FBG-FPF) is achieved with a laser output power of similar to 4 mW, when the pump power is similar to 75 mW.
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| 2. |
- Liu, Lihong, et al.
(författare)
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Development of a primer-probe energy transfer real-time PCR assay for improved detection of classical swine fever virus
- 2009
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Ingår i: Journal of Virological Methods. - : Elsevier BV. - 0166-0934 .- 1879-0984. ; 160, s. 69-73
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Tidskriftsartikel (refereegranskat)abstract
- Classical swine fever (CSF) is a contagious and devastating disease, causing serious losses in the pig industry worldwide. Vaccination of pigs with the conventional C-strain vaccine has been practised in different regions of the world in order to prevent the disease. In the control programmes of CSF, rapid detection and identification of the causing agent, classical swine fever virus (CSFV) is a crucial step. This study describes a novel real-time PCR assay based on primer-probe energy transfer (PriProET) technology for improved detection of CSFV. The assay is able to detect 20 copies of viral cDNA per reaction, showing a high sensitivity. The specificity has been evaluated by testing 57 pestiviruses, representing all species and unclassified pestiviruses. The assay has been found to be highly reproducible. Following PCR amplification, melting curve analysis allows confirmation of specific amplicons, and differentiation between wild-type CSFV and certain C-strain vaccines. This study provides a new tool for the diagnosis of CSF. (C) 2009 Elsevier B.V. All rights reserved.
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| 3. |
- Liu, Lihong, et al.
(författare)
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Maximum likelihood and Bayesian analyses of a combined nucleotide sequence dataset for genetic characterization of a novel pestivirus, SVA/cont-08
- 2009
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Ingår i: Archives of Virology. - : Springer Science and Business Media LLC. - 0304-8608 .- 1432-8798. ; 154, s. 1111-1116
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Tidskriftsartikel (refereegranskat)abstract
- Bovine viral diarrhoea virus 1 (BVDV-1) and Bovine viral diarrhoea virus 2 (BVDV-2) are two recognised bovine pestivirus species of the genus Pestivirus. Recently, a pestivirus, termed SVA/cont-08, was detected in a batch of contaminated foetal calf serum originating from South America. Comparative sequence analysis showed that the SVA/cont-08 virus shares 15-28% higher sequence identity to pestivirus D32/00_'HoBi' than to members of BVDV-1 and BVDV-2. In order to reveal the phylogenetic relationship of SVA/cont-08 with other pestiviruses, a molecular dataset of 30 pestiviruses and 1,896 characters, comprising the 5'UTR, N(pro) and E2 gene regions, was analysed by two methods: maximum likelihood and Bayesian approach. An identical, well-supported tree topology was observed, where four pestiviruses (SVA/cont-08, D32/00_'HoBi', CH-KaHo/cont, and Th/04_KhonKaen) formed a monophyletic clade that is closely related to the BVDV-1 and BVDV-2 clades. The strategy applied in this study is useful for classifying novel pestiviruses in the future.
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| 4. |
- Liu, Lihong, et al.
(författare)
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Phylogeny, classification and evolutionary insights into pestiviruses
- 2009
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Ingår i: Virology. - : Elsevier BV. - 0042-6822 .- 1178-122X. ; 385, s. 351-357
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Tidskriftsartikel (refereegranskat)abstract
- The genus Pestivirus comprises four established species: Bovine viral diarrhoea viruses 1 (BVDV-1) and 2 (BVDV-2), Border disease virus (BDV), and Classical swine fever virus (CSFV); and a tentative species, Pestivirus of giraffe. Additional pestiviruses have been identified and suggested for recognition as novel subgroups/species. To achieve a reliable phylogeny as the basis for classification of pestiviruses, a molecular dataset of 56 pestiviruses and 2089 characters, comprising the 5'UTR, complete N(pro) and E2 gene regions was analysed by Maximum likelihood and Bayesian approach. An identical, robust tree topology was inferred, where seven well-supported monophyletic clades and two highly divergent lineages were identified. Dating most recent common ancestor was estimated for major pestivirus lineages and their evolutionary histories were revealed. Accordingly, a new proposal is presented for the classification of pestiviruses into nine species: BVDV-1, BVDV-2, BVDV-3 (atypical bovine pestiviruses), Pestivirus of giraffe, CSFV, BDV, Tunisian sheep Virus (TSV; previously termed "Tunisian isolates"), Antelope and Bungowannah. (c) 2008 Elsevier Inc. All rights reserved.
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