| 1. |
- Bernacka Wojcik, Iwona, et al.
(författare)
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Flexible Organic Electronic Ion Pump for Flow-Free Phytohormone Delivery into Vasculature of Intact Plants
- 2023
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Ingår i: Advanced Science. - : WILEY. - 2198-3844. ; 10:14
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Tidskriftsartikel (refereegranskat)abstract
- Plant vasculature transports molecules that play a crucial role in plant signaling including systemic responses and acclimation to diverse environmental conditions. Targeted controlled delivery of molecules to the vascular tissue can be a biomimetic way to induce long distance responses, providing a new tool for the fundamental studies and engineering of stress-tolerant plants. Here, a flexible organic electronic ion pump, an electrophoretic delivery device, for controlled delivery of phytohormones directly in plant vascular tissue is developed. The c-OEIP is based on polyimide-coated glass capillaries that significantly enhance the mechanical robustness of these microscale devices while being minimally disruptive for the plant. The polyelectrolyte channel is based on low-cost and commercially available precursors that can be photocured with blue light, establishing much cheaper and safer system than the state-of-the-art. To trigger OEIP-induced plant response, the phytohormone abscisic acid (ABA) in the petiole of intact Arabidopsis plants is delivered. ABA is one of the main phytohormones involved in plant stress responses and induces stomata closure under drought conditions to reduce water loss and prevent wilting. The OEIP-mediated ABA delivery triggered fast and long-lasting stomata closure far away from the delivery point demonstrating systemic vascular transport of the delivered ABA, verified delivering deuterium-labeled ABA.
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| 2. |
- Axelsson, Louise, et al.
(författare)
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The meaning of animal well-being- for farmers and dairy farm employees
- 2024
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Ingår i: Agricultural and Food Science. - 1459-6067 .- 1795-1895. ; 33, s. 30-39
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Tidskriftsartikel (refereegranskat)abstract
- Dairy farms in Sweden have undergone a structural change. The number of family farms has decreased, while the number of large dairy farms with employees caring for the animals has increased. This changing situation has created a new farming landscape. From that perspective, it is crucial to gain insight into what factors contribute to the well-being of humans and animals on big dairy farms. Twenty-three semi-structured interviews were conducted on three farms. Farmers and employees were interviewed. The material was analysed using a qualitative approach inspired by qualitative content analysis. For the farmers and employees, animal well-being was central for various reasons and from different perspectives. Despite the differences, the impact of animal well-being was interlinked between the two groups. An increased and deeper understanding of the different perspectives and needs arising from the different roles of farmers and employees can provide new knowledge about factors important for improvinganimal well-being.
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| 3. |
- Axelsson, Robert, et al.
(författare)
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The Challenge of Transdisciplinary Research : A Case Study of Learning by Evaluation for Sustainable Transport Infrastructures
- 2020
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Ingår i: Sustainability. - : MDPI. - 2071-1050. ; 12:17, s. 1-24
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Tidskriftsartikel (refereegranskat)abstract
- While transdisciplinary (TD) research is desired in order to solve real world sustainability issues, this may be challenging for both academic and non-academic participants. Supporting learning through evaluation, we analyzed a project aiming at sustainable transport infrastructures. After developing a TD research framework as a benchmark, two external independent evaluators interviewed all project researchers, representatives for end-users, and donors. The evaluators compared results with the framework, and evaluators and participants critically reflected on the results together. There were three inconsistencies relative to the framework: (1) limited understanding of TD research among project management, end-users, and most of the researchers; (2) no structured learning process among end-users; instead, they expressed very diverse opinions about what they expected from the project; (3) project leaders had limited understanding of the special challenges of TD research, did not fully understand the status of the project's social system, and thus did not act as facilitators of the required collaborative learning process. Non-academic participants saw themselves as customers and not as partners in the knowledge production process. We conclude that TD problem-solving research requires much time and needs facilitation and training. A preparatory phase with a lower level of funding would be helpful in preparing for TD processes.
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| 4. |
- Blennow Nordström, Erik, et al.
(författare)
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Neuropsychological outcome after cardiac arrest : results from a sub-study of the targeted hypothermia versus targeted normothermia after out-of-hospital cardiac arrest (TTM2) trial
- 2023
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Ingår i: Critical Care. - : BioMed Central (BMC). - 1364-8535 .- 1466-609X. ; 27:1
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Cognitive impairment is common following out-of-hospital cardiac arrest (OHCA), but the nature of the impairment is poorly understood. Our objective was to describe cognitive impairment in OHCA survivors, with the hypothesis that OHCA survivors would perform significantly worse on neuropsychological tests of cognition than controls with acute myocardial infarction (MI). Another aim was to investigate the relationship between cognitive performance and the associated factors of emotional problems, fatigue, insomnia, and cardiovascular risk factors following OHCA.METHODS: This was a prospective case-control sub-study of The Targeted Hypothermia versus Targeted Normothermia after Out-of-Hospital Cardiac Arrest (TTM2) trial. Eight of 61 TTM2-sites in Sweden, Denmark, and the United Kingdom included adults with OHCA of presumed cardiac or unknown cause. A matched non-arrest control group with acute MI was recruited. At approximately 7 months post-event, we administered an extensive neuropsychological test battery and questionnaires on anxiety, depression, fatigue, and insomnia, and collected information on the cardiovascular risk factors hypertension and diabetes.RESULTS: Of 184 eligible OHCA survivors, 108 were included, with 92 MI controls enrolled. Amongst OHCA survivors, 29% performed z-score ≤ - 1 (at least borderline-mild impairment) in ≥ 2 cognitive domains, 14% performed z-score ≤ - 2 (major impairment) in ≥ 1 cognitive domain while 54% performed without impairment in any domain. Impairment was most pronounced in episodic memory, executive functions, and processing speed. OHCA survivors performed significantly worse than MI controls in episodic memory (mean difference, MD = - 0.37, 95% confidence intervals [- 0.61, - 0.12]), verbal (MD = - 0.34 [- 0.62, - 0.07]), and visual/constructive functions (MD = - 0.26 [- 0.47, - 0.04]) on linear regressions adjusted for educational attainment and sex. When additionally adjusting for anxiety, depression, fatigue, insomnia, hypertension, and diabetes, executive functions (MD = - 0.44 [- 0.82, - 0.06]) were also worse following OHCA. Diabetes, symptoms of anxiety, depression, and fatigue were significantly associated with worse cognitive performance.CONCLUSIONS: In our study population, cognitive impairment was generally mild following OHCA. OHCA survivors performed worse than MI controls in 3 of 6 domains. These results support current guidelines that a post-OHCA follow-up service should screen for cognitive impairment, emotional problems, and fatigue.TRIAL REGISTRATION: ClinicalTrials.gov, NCT03543371. Registered 1 June 2018.
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| 5. |
- Blennow Nordström, Erik, et al.
(författare)
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Neuropsychological outcome after cardiac arrest : A prospective case control sub-study of the Targeted hypothermia versus targeted normothermia after out-of-hospital cardiac arrest trial (TTM2)
- 2020
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Ingår i: BMC Cardiovascular Disorders. - : Springer Science and Business Media LLC. - 1471-2261. ; 20:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: This study is designed to provide detailed knowledge on cognitive impairment after out-of-hospital cardiac arrest (OHCA) and its relation to associated factors, and to validate the neurocognitive screening of the Targeted Hypothermia versus Targeted Normothermia after Out-of-Hospital Cardiac Arrest trial (TTM2-trial), assessing effectiveness of targeted temperature management after OHCA. Methods: This longitudinal multi-center clinical study is a sub-study of the TTM2-trial, in which a comprehensive neuropsychological examination is performed in addition to the main TTM2-trial neurocognitive screening. Approximately 7 and 24 months after OHCA, survivors at selected study sites are invited to a standardized assessment, including performance-based tests of cognition and questionnaires of emotional problems, fatigue, executive function and insomnia. At 1:1 ratio, a matched control group from a cohort of acute myocardial infarction (MI) patients is recruited to perform the same assessment. We aim to include 100 patients per group. Potential differences between the OHCA patients and the MI controls at 7 and 24 months will be analyzed with a linear regression, using composite z-scores per cognitive domain (verbal, visual/constructive, working memory, episodic memory, processing speed, executive functions) as primary outcome measures. Results from OHCA survivors on the main TTM2-trial neurocognitive screening battery will be compared with neuropsychological test results at 7 months, using sensitivity and specificity analyses. Discussion: In this study we collect detailed information on cognitive impairment after OHCA and compare this to a control group of patients with acute MI. The validation of the TTM2 neurocognitive screening battery could justify its inclusion in routine follow-up. Our results may have a potential to impact on the design of future follow-up strategies and interventions after OHCA. Trial registration: ClinicalTrials.gov, NCT03543371. Registered 1 June 2018
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| 6. |
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| 7. |
- Blix Germundsson, Lisa, et al.
(författare)
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Collective agency as a leverage point in multi-actor innovation: Two case studies from Swedish horticulture
- 2023
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Ingår i: Agricultural and Food Science. - 1459-6067 .- 1795-1895. ; 32, s. 166-178
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study is to investigate how horticultural firms meet their need for innovation within the innovation system. A comparative process ethnography approach was applied to two cases of long-term collaboration between multiple actors in Swedish horticulture. The retrospective reconstruction of the cases through documentation and interviews allowed for triangulation of data in a grounded theory approach. The leverage points of the developing collaborative processes were found to be; having agency and a network approach, the forming of collective agency through social learning, enhancing resource access, and operationalization of results. The concept of leverage points is useful for understanding evolutionary changes in multi-actor collaborations. The use of the concept of collective agency contributes to the understanding of the dynamics of demand articulation, illustrating a reciprocal process between the actors' individual agencies, and evolving over time through their social learning. The results point to a need for policymakers and practitioners to develop a broader understanding of how actors work in the innovation system to enhance knowledge development and innovation.
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| 8. |
- Blix Germundsson, Lisa, et al.
(författare)
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Leverage points in farmer, advisor and researcher interactions.
- 2023
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this study is to investigate how agricultural firms can use innovation support services to develop new knowledge and innovation, for their sustainable business development and growth. The methods comprise a qualitative case study with a comparative process ethnography approach, employing two cases of long-term collaborations between multiple actors in Sweden. The findings suggest that the processes of social learning, the forming of collective agency, enhancing of resource access and the operationalizing of results, were leverage points creating the ability to maintain and develop the collaboration over time. The practical implications include how agricultural firms can gain innovative strength and find leverage by forming collective agency with key individuals in order to access complementary competences and resources of others. The theoretical implications include the value of collective agency for multi-actor collaborations, and that a composition of smaller leverage points were found to enable larger change.
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| 9. |
- Krafft, Jannica, et al.
(författare)
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Delivering too much, too little or off target-possible consequences of differences in perceptions on agricultural advisory services
- 2021
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Ingår i: Agriculture and Human Values. - : Springer Science and Business Media LLC. - 0889-048X .- 1572-8366. ; 39, s. 185-199
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Tidskriftsartikel (refereegranskat)abstract
- Advisory services are considered to play an important role in the development of competitiveness and sustainability in agriculture. Advisory services have been studied at policy level, structural level and within case studies, but there is still restricted knowledge about advisors' and farmers' view on advisory services in general. This paper presents the views of Swedish advisors and farmers on advisory services. In a survey-based study, perceptions of farm advisors and full-time farmers in commercial Swedish agriculture on advisory services were identified and statistically analysed, comparing differences between and within the groups. The results are structured around three main themes; motives for a farmer using or not using advisory services, preferred approach by the advisor and future demands on advisory services and their importance today. Possible consequences of differences in perceptions for on-farm service delivery were assessed. Similarities in perceptions on advisory services among advisors and farmers, were found in areas characterised by well-defined questions or production-related issues. Significant differences in perceptions of advisors and farmers emerged in less concrete areas and on topics connected to change, management and strategy. Consequences of discrepancies in perceptions are that advisors may deliver too much, too little or off target, especially when expectations on advisory services are not clearly expressed. A strong and proactive back-office supporting the advisors is needed to prevent these possible consequences.
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| 10. |
- Lagercrantz, Ulf, et al.
(författare)
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Nyctinastic thallus movement in the liverwort Marchantia polymorpha is regulated by a circadian clock
- 2020
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- The circadian clock coordinates an organism’s growth, development and physiology with environmental factors. One illuminating example is the rhythmic growth of hypocotyls and cotyledons in Arabidopsis thaliana. Such daily oscillations in leaf position are often referred to as sleep movements or nyctinasty. Here, we report that plantlets of the liverwort Marchantia polymorpha show analogous rhythmic movements of thallus lobes, and that the circadian clock controls this rhythm, with auxin a likely output pathway affecting these movements. The mechanisms of this circadian clock are partly conserved as compared to angiosperms, with homologs to the core clock genes PRR, RVE and TOC1 forming a core transcriptional feedback loop also in M. polymorpha.
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