Single-step purification of sIgA from bovine colostrums by anion-exchange chromatography

• A fraction containing sIgA(sIgA-rich fraction)was prepared from bovine colostrum by anion exchange chromatography using alkali resin. The effect of changing buffer properties(pH and ionic strength)on purity of sIgA was studied. The best result was sIgA purity increasing from 16.31% in bovine colostrum solution to 91.24% in the eluting fraction with a recovery of 47% at the condition of pH 7.0,0.03mol/L sodium phosphate. These results suggested that the anion exchange chromatography using alkali resin was a potential process for sIgA purification from bovine colostrum.

Nyckelord

food enginnering

protein separation

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ref (ämneskategori)

art (ämneskategori)