| 1. |
- Larsson, Inger, et al.
(författare)
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Interferon production in glia and glioma cell lines
- 1978
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Ingår i: Infection and Immunity. - 1098-5522. ; 22:3, s. 786-789
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Tidskriftsartikel (refereegranskat)abstract
- Interferon (IF) was produced in glia and glioma cell lines in titers comparable to those produced by human fibroblasts. It was inducible by both Sendai virus and polyriboinosinic:polyribocytidylic acid. "Superinduction" resulted in up to 500-fold-higher titers of IF. The IF appeared to be of the fibroblast type, as revealed by experiments using heat treatment, assay of antiviral activity in heterologous cell lines, and neutralization with specific antisera. Since large amounts of IF may easily be produced with glioma cell lines, such cells may be suitable for mass production of IF.
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| 2. |
- Miörner, Håkan, et al.
(författare)
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Regulation of mitogen-induced lymphocyte DNA synthesis by human interferon of different origins
- 1978
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 35:1, s. 15-24
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Tidskriftsartikel (refereegranskat)abstract
- Human fibroblast and leukocyte interferons were found to suppress lymphocyte mitogenesis induced by optimal doses of phytohemagglutinin and concanavalin A. In certain situations (low doses of mitogen and/or low doses of interferon), however, interferon significantly enhanced mitogenesis. In experiments using varying concentrations of interferon, dose-response curves with different slopes were obtained for fibroblast and leukocyte interferons. The effect of interferon was apparently exerted during early stages of the lymphocyte cell cycle. There was no inhibitory effect of interferon if the lymphocytes were washed with medium before being exposed to mitogen. Interferon increased the binding of radiolabeled mitogens to cells. The results suggest that the immunological effects of interferon are consequences of actions on lymphoid cells. Fibroblast and leukocyte interferons seem to have different modes of action, or to bind differently to target cells. Possible mechanisms for the suppressive and enhancing effects of interferons on lymphoid cells are discussed.
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| 3. |
- Lundgren, Erik, 1942-
(författare)
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Amino acid naphthylamidase isozymes in human cells grown in vitro : Hormonal regulation and isozyme differentiation in cancer cells and normal cells
- 1972
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The elucidation of regulatory mechanisms in higher organisms represents a front line problem in biochemical genetics. In Man the only material available for experimental studies of regulatory mechanisms is cells cultured in vitro. Enzymes which are differentiated into isozymes may have a complexgenetic background involving the action of more than one gene locus. The study of isozyme systems in cultured cells has developed into a valuable tool of increasing importance for the understanding of the genetic regulatorymechanisms in normal cells as well as in cancer cells.The purposes of this investigation were:1. to elucidate the isozyme differentiation of amino acid naphthylamidasein cultured human cancer cells and normal cells.2. to study the regulatory effects of steroid hormones especially hydrocortisoneon the levels of the different isozymes.
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| 4. |
- Strannegård, Örjan, et al.
(författare)
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Modulation of immune responses in newborn and adult mice by interferon
- 1978
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Ingår i: Infection and Immunity. - 1098-5522. ; 20:2, s. 334-339
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Tidskriftsartikel (refereegranskat)abstract
- Interferon was found to have both suppressive and enhancing effects on the antibody response in newborn and adult mice. Evidence was obtained that these effects are primarily evoked during the initial steps controlling cell proliferation. Stimulation of thymus and spleen cells with a T-cell mitogen was enhanced by low doses and suppressed by high doses of interferon. Treatment of parental spleen cells with interferon before injecting them into immunized F1 hybrid mice resulted in an enhanced allogeneic effect. These results are compatible with the hypothesis that interferon affects T cells and has an immunoregulatory role, either by inhibiting the action of suppressor cells or by promoting immunological maturation.
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