| 1. |
- Birney, Ewan, et al.
(författare)
-
Prepublication data sharing
- 2009
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 461:7261, s. 168-170
-
Tidskriftsartikel (refereegranskat)abstract
- Rapid release of prepublication data has served the field of genomics well. Attendees at a workshop in Toronto recommend extending the practice to other biological data sets.
|
|
| 2. |
- Amirahmadi, S. F., et al.
(författare)
-
Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells
- 2005
-
Ingår i: Arthritis and Rheumatism. - : Wiley. - 0004-3591 .- 1529-0131. ; 52:6, s. 1897-1906
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Some monoclonal antibodies (mAb) to type II collagen (CII) are arthritogenic upon passive transfer to mice. We undertook this study to investigate whether such mAb are pathogenic in the absence of mediators of inflammation. METHODS: The arthritogenic mAb CIIC1 and M2139, and the nonarthritogenic mAb CIIF4, each reactive with a distinct and well-defined conformational epitope on CII, were compared with control mAb GAD6. Bovine chondrocytes were cultured with one of the mAb, and on days 3, 6, and 9, antibody binding by chondrocytes and newly synthesized extracellular matrix (ECM) was examined by immunofluorescence, morphologic effects were studied by electron microscopy, and synthesis of matrix components was determined by metabolic labeling with (3)H-proline for collagen and (35)S-sulfate for proteoglycans. RESULTS: All 3 mAb to CII bound to the matrix. CIIC1 and M2139 adversely affected the cultures, whereas CIIF4 did not. CIIC1 caused disorganization of CII fibrils in the ECM without affecting chondrocyte morphology, and increased matrix synthesis. M2139 caused thickening and aggregation of CII fibrils in the ECM and abnormal chondrocyte morphology but matrix synthesis was unaffected. CONCLUSION: The unique arthritogenic capacity of particular anti-CII mAb upon passive transfer could be explained by their adverse, albeit differing, effects in primary cultures of chondrocytes. Such effects occur independent of inflammation mediators and are related to the epitope specificity of the mAb. Interference with the structural integrity of CII could precede, and even initiate, the inflammatory expression of disease.
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Hammarlund, Dan, et al.
(författare)
-
A sedimentary record of the rise and fall of the metal industry in Bergslagen, south central Sweden
- 2008
-
Ingår i: Journal of Paleolimnology. - : Springer Science and Business Media LLC. - 0921-2728 .- 1573-0417. ; 39:4, s. 463-475
-
Tidskriftsartikel (refereegranskat)abstract
- Detailed stratigraphic analyses of sediments deposited in Lake Botjarnen, a small boreal forest lake in the shield terrain of central Sweden, clearly reflect progressively increasing human impact on terrestrial and aquatic ecosystems following settlement and establishment of an iron industry in the 17th century. Rising frequencies of pollen and spores from light-demanding plants provide evidence of extensive forest clearance for charcoal and timber production, which peaked in the early 20th century. An associated increase in catchment erosion is reflected by changing carbon and nitrogen elemental content and carbon-isotope composition of sediment organic matter and by increased magnetic susceptibility of the sediments. Records of air-borne pollutants (lead, zinc and sulphur) can be correlated to the development of local and regional mining and metal industry as inferred from historical accounts. Rapid recession of the iron industry led to re-forestation and recovery of the aquatic nutrient status to pre-industrial conditions over the past 100 years. The chronology of the sediment succession, which is based on Pb-210 and Cs-137 radionuclide data in combination with radiocarbon dating, is confirmed by historical lead pollution trends established for the region.
|
|
