| 1. |
|
|
| 2. |
- Lenshof, Andreas, et al.
(författare)
-
Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics.
- 2009
-
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 81:Jul 13, s. 6030-6037
-
Tidskriftsartikel (refereegranskat)abstract
- The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL).
|
|
| 3. |
- Lenshof, Andreas, et al.
(författare)
-
Acoustic whole blood plasmapheresis chip for PSA microarray diagnostics
- 2009
-
Ingår i: Proceedings of Conference, MicroTAS 2009 : The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 1330-1332
-
Konferensbidrag (refereegranskat)abstract
- An acoustic device for generating high quality blood plasma for PSA microarray diagnostics is presented.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
- Malm, Johan, et al.
(författare)
-
The pentraxin serum amyloid P component is found in the male genital tract and attached to spermatozoa
- 2008
-
Ingår i: International Journal of Andrology. - : Wiley. - 0105-6263 .- 1365-2605. ; 31, s. 508-517
-
Tidskriftsartikel (refereegranskat)abstract
- Serum amyloid P component (SAP) belongs to the pentraxin family of proteins, members of which are characterized by radial pentameric structure and calcium-dependent ligand binding. SAP is present in all types of amyloidosis and has been shown to bind to several ligands, but the physiological function of this protein has not been fully elucidated. The present study identified and characterized SAP in human semen and immunolocalized it to the male reproductive tract. SAP was also detected in seminal plasma by immunoblotting and purification by affinity chromatography followed by mass spectrometry. According to electroimmunoassay, the concentration of SAP in semen is approximately 2 mg/L, and flow cytometry revealed SAP attached to the surface of spermatozoa. Moreover, immunohistochemistry showed positive staining of spermatozoa, subsets of epithelial cells, and the stroma of accessory male genital glands and testis. Presence of mRNA supports local production of SAP, as shown with reverse transcription polymerase chain reaction. We identified SAP in a new setting - the human male reproductive system. SAP was detected on ejaculated spermatozoa, in seminal plasma and in tissue sections from the male reproductive tract. Further functional studies are needed to explain the role of SAP in human reproduction.
|
|
| 7. |
- Malm, Richard, et al.
(författare)
-
Field testing and simulation of dynamic properties of a tied arch railway bridge
- 2006
-
Ingår i: Engineering structures. - : Elsevier BV. - 0141-0296 .- 1873-7323. ; 28:1, s. 143-152
-
Tidskriftsartikel (refereegranskat)abstract
- This paper deals with the dynamic effects on a tied arch railway bridge during train passages. The bridge is located in Ange municipality in central Sweden. Large vibrations of the hangers were observed during train passages and field measurements have been performed to study the train induced vibrations of the hangers. According to the Palmgren-Miners linear damage rule, there is a great risk of fatigue in the threaded parts of the hangers. The low damping in the hangers has a large influence of the risk of fatigue failure. A 3D finite element model of the bridge has been developed where the dynamic vehicle-bridge interaction was modelled using contact surface formulation with a sprung mass train system. The measured data results are compared with the results from the FE model to give a better understanding of the dynamic behaviour of the bridge.
|
|
| 8. |
- Malm, T M, et al.
(författare)
-
Bone-marrow-derived cells contribute to the recruitment of microglial cells in response to beta-amyloid deposition in APP/PS1 double transgenic Alzheimer mice
- 2005
-
Ingår i: Neurobiology of Disease. - : Elsevier BV. - 0969-9961. ; 18:1, s. 134-142
-
Tidskriftsartikel (refereegranskat)abstract
- The role of microglia recruited from bone marrow (BM) into the CNS during the progression of Alzheimer's disease (AD) is poorly understood. To investigate whether beta-amyloid (Abeta) associated microglia are derived from blood monocytes, we transplanted BM cells from enhanced green fluorescent protein expressing mice into young or old transgenic AD mice and determined the engraftment of BM-derived cells into the brain and their relative distribution near Abeta deposits. When young transgenic mice were transplanted before the onset of AD-like pathology and the brains analyzed 6.5 months later, the number of engrafted cells was significantly higher than in age-matched wild type mice. Moreover, the number of BM-derived cells associated with Abeta was significantly higher than in old transgenic mice transplanted after the establishment of AD-like pathology. Local inflammation caused by intrahippocampal lipopolysaccharide injection significantly increased the engraftment of BM-derived cells in old AD mice and decreased the hippocampal Abeta burden. These results suggest that infiltration of BM-derived monocytic cells into the brain contributes to the development of microglial reaction in AD.
|
|
| 9. |
|
|
