| 1. |
- Mikkonen, Saara, et al.
(författare)
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Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation
- 2022
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Ingår i: Biotechnology Journal. - : Wiley. - 1860-6768 .- 1860-7314.
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Tidskriftsartikel (refereegranskat)abstract
- The increased use of biopharmaceuticals calls for improved means of bioprocess monitoring. In this work, capillary electrophoresis (CE) and microchip electrophoresis (MCE) methods were developed and applied for the analysis of amino acids (AAs) in cell culture supernatant. In samples from different days of a Chinese hamster ovary cell cultivation process, all 19 proteinogenic AAs containing primary amine groups could be detected using CE, and 17 out of 19 AAs using MCE. The relative concentration changes in different samples agreed well with those measured by high-performance liquid chromatography (HPLC). Compared to the more commonly employed HPLC analysis, the CE and MCE methods resulted in faster analysis, while significantly lowering both the sample and reagent consumption, and the cost per analysis.
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| 2. |
- van der Burg, D., et al.
(författare)
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Method development for mono- and disaccharides monitoring in cell culture medium by capillary and microchip electrophoresis
- 2022
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 43:9-10, s. 922-929
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Tidskriftsartikel (refereegranskat)abstract
- The rapidly growing, competitive biopharmaceutical market requires tight bioprocess monitoring. An integrated, automated platform for the routine online/at-line monitoring of key factors in the cell culture medium could greatly improve process monitoring. Mono- and disaccharides, as the main energy and carbon source, are one of these key factors. A CE-LIF method was developed for the analysis of several mono- and disaccharides, considering requirements and restrictions for analysis in an integrated, automated monitoring platform, such as the possibility for miniaturization to microchip electrophoresis. Analysis was performed after fluorescent derivatization with 8-aminopyrene-1,3,6-trisulfonic acid. The derivatisation reaction and the separation BGE were optimized using design of experiments. The developed method is applicable to the complex matrix of cell culture medium and proved transferable to microchip electrophoresis.
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