| 1. |
- Suarez, Carolina, et al.
(författare)
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Metagenomic evidence of a novel family of anammox bacteria in a subsea environment
- 2022
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Ingår i: Environmental Microbiology. - : Wiley. - 1462-2912 .- 1462-2920. ; 24:5, s. 2348-2360
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Tidskriftsartikel (refereegranskat)abstract
- Bacteria in the order 'Candidatus Brocadiales' within the phylum Planctomycetes (Planctomycetota) have the remarkable ability to perform anaerobic ammonium oxidation (anammox). Two families of anammox bacteria with different biogeographical distributions have been reported, marine Ca. Scalinduaceae and freshwater Ca. Brocadiaceae. Here we report evidence of three new species within a novel genus and family of anammox bacteria, which were discovered in biofilms of a subsea road tunnel under a fjord in Norway. In this particular ecosystem, the nitrogen cycle is likely fuelled by ammonia from organic matter degradation in the fjord sediments and the rock mass above the tunnel, resulting in the growth of biofilms where anammox bacteria can thrive under oxygen limitation. We resolved several metagenome-assembled genomes (MAGs) of anammox bacteria, including three Ca. Brocadiales MAGs that could not be classified at the family level. MAGs of this novel family had all the diagnostic genes for a full anaerobic ammonium oxidation pathway in which nitrite was probably reduced by a NirK-like reductase. A survey of published molecular data indicated that this new family of anammox bacteria occurs in many marine sediments, where its members presumably would contribute to nitrogen loss.
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| 2. |
- Axman, Erik, et al.
(författare)
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Assessing the Validity and Cover Rate of the National Swedish Hernia Register
- 2021
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Ingår i: CLINICAL EPIDEMIOLOGY. - : Dove Press. - 1179-1349. ; 13, s. 1129-1134
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To assess the validity and cover rate of the Swedish hernia register. Material and Methods: Since the start of the Swedish Hernia register an annual review of randomly selected hospitals has been carried out, and since 2013 in a more standardized form to allow a systematic data collection and evaluation. 10% of all clinics were randomly selected each year in a specific region of Sweden, ensuring a systematic validation of all regions from north to south. Data from 2013 to 2018 were analyzed regarding data quality and from 2014 to 2018 regarding cover rate. All operations registered at the validated clinics were compared with the Swedish Hernia Register to assess cover rate. Fifty operations were randomly selected at each clinic and data in the Swedish Hernia register were compared with the medical records to evaluate data quality. Results: Fifty-five clinics was evaluated and a total of 73,764 variables were compared with the medical records. Cover rate between 2014 and 2018 was 97%. The proportion of correct variables was 98% between 2013 and 2018. Most frequent errors were ASA score, date at which the patient was put on the waiting list and postoperative complications. Conclusion: This unique validation of a national hernia register shows a high cover rate and good quality of data. Efforts to maintain and improve national registers are of great importance. Research with data from the Swedish hernia register should be evaluated on the basis of the results presented in this study.
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| 3. |
- Bauer, M., et al.
(författare)
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Semi-invariant Riemannian metrics in hydrodynamics
- 2020
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Ingår i: Calculus of Variations and Partial Differential Equations. - : Springer Science and Business Media LLC. - 0944-2669 .- 1432-0835. ; 59:2
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Tidskriftsartikel (refereegranskat)abstract
- Many models in mathematical physics are given as non-linear partial differential equation of hydrodynamic type; the incompressible Euler, KdV, and Camassa-Holm equations are well-studied examples. A beautiful approach to well-posedness is to go from the Eulerian to a Lagrangian description. Geometrically it corresponds to a geodesic initial value problem on the infinite-dimensional group of diffeomorphisms with a right invariant Riemannian metric. By establishing regularity properties of the Riemannian spray one can then obtain local, and sometimes global, existence and uniqueness results. There are, however, many hydrodynamic-type equations, notably shallow water models and compressible Euler equations, where the underlying infinite-dimensional Riemannian structure is not fully right invariant, but still semi-invariant with respect to the subgroup of volume preserving diffeomorphisms. Here we study such metrics. For semi-invariant metrics of Sobolev Hk-type we give local and some global well-posedness results for the geodesic initial value problem. We also give results in the presence of a potential functional (corresponding to the fluid's internal energy). Our study reveals many pitfalls in going from fully right invariant to semi-invariant Sobolev metrics; the regularity requirements, for example, are higher. Nevertheless the key results, such as no loss or gain in regularity along geodesics, can be adopted.
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| 4. |
- Cifani, P., et al.
(författare)
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Casimir preserving spectrum of two-dimensional turbulence
- 2022
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Ingår i: Physical Review Fluids. - 2469-990X. ; 7:8
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Tidskriftsartikel (refereegranskat)abstract
- We present predictions of the energy spectrum of forced two-dimensional turbulence obtained by employing a structure-preserving integrator. In particular, we construct a finite-mode approximation of the Navier-Stokes equations on the unit sphere, which, in the limit of vanishing viscosity, preserves the Lie-Poisson structure. As a result, integrated powers of vorticity are conserved in the inviscid limit. We obtain robust evidence for the existence of the double energy cascade, including the formation of the -3 scaling of the inertial range of the direct cascade. We show that this can be achieved at modest resolutions compared to those required by traditional numerical methods.
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| 5. |
- Ekholm, Jennifer, et al.
(författare)
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Full-scale aerobic granular sludge for municipal wastewater treatment – granule formation, microbial succession, and process performance
- 2022
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Ingår i: Environmental Science: Water Research & Technology. - 2053-1419. ; :8, s. 3138-3154
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Tidskriftsartikel (refereegranskat)abstract
- Aerobic granular sludge (AGS) plants have gained growing interest and application due to their low energy demand, small footprint, and low operational costs. However, the fulfilment of strict discharge limits for nitrogen and phosphorus, vast seasonal temperature variations, and large peaks in influent flows may pose challenges to the implementation of AGS. Moreover, the knowledge about microbial community assembly and process performance under varying environmental conditions in full-scale reactors is still limited. In this study, the first implementation of the AGS process in the Nordic countries was assessed. In two full-scale AGS reactors with different seeding sludges, the start-up was associated with rapid changes in microbial community composition in both, but only successful granulation in one. As a consequence, the non-granulated reactor was eventually reseeded with biomass from the better granulated reactor. This resulted in a convergence of the microbial communities in the two reactors with the maintenance of stable sludge concentrations (6–8 g L−1) with large granules (50–80% with diameter >2 mm) and fast settling of biomass (SVI30/SVI10 of 0.9–1). Immigration from the influent wastewater was a minor factor affecting the microbial community once the granules had formed, while the seasonal variations in environmental factors were identified as important. Key guilds of AOB (Nitrosomonas), NOB (mainly Ca. Nitrotoga), PAOs (mainly Tetrasphaera), and GAOs (mainly Ca. Competibacter) varied considerably in abundance throughout the study period. After 15 months, stable organic matter, nitrogen, and phosphorus removal were attained with low effluent concentrations. During the start-up, the BOD7/N ratio, influent flow, and temperature were important factors influencing the performance of the AGS.
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| 6. |
- Ekholm, Jennifer, 1992, et al.
(författare)
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Full-scale aerobic granular sludge for municipal wastewater treatment - granule formation, microbial succession, and process performance
- 2022
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Ingår i: Environmental Science: Water Research and Technology. - : Royal Society of Chemistry (RSC). - 2053-1419 .- 2053-1400. ; 8:12, s. 3138-3154
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Tidskriftsartikel (refereegranskat)abstract
- Aerobic granular sludge (AGS) plants have gained growing interest and application due to their low energy demand, small footprint, and low operational costs. However, the fulfilment of strict discharge limits for nitrogen and phosphorus, vast seasonal temperature variations, and large peaks in influent flows may pose challenges to the implementation of AGS. Moreover, the knowledge about microbial community assembly and process performance under varying environmental conditions in full-scale reactors is still limited. In this study, the first implementation of the AGS process in the Nordic countries was assessed. In two full-scale AGS reactors with different seeding sludges, the start-up was associated with rapid changes in microbial community composition in both, but only successful granulation in one. As a consequence, the non-granulated reactor was eventually reseeded with biomass from the better granulated reactor. This resulted in a convergence of the microbial communities in the two reactors with the maintenance of stable sludge concentrations (6-8 g L-1) with large granules (50-80% with diameter >2 mm) and fast settling of biomass (SVI30/SVI10 of 0.9-1). Immigration from the influent wastewater was a minor factor affecting the microbial community once the granules had formed, while the seasonal variations in environmental factors were identified as important. Key guilds of AOB (Nitrosomonas), NOB (mainly Ca. Nitrotoga), PAOs (mainly Tetrasphaera), and GAOs (mainly Ca. Competibacter) varied considerably in abundance throughout the study period. After 15 months, stable organic matter, nitrogen, and phosphorus removal were attained with low effluent concentrations. During the start-up, the BOD7/N ratio, influent flow, and temperature were important factors influencing the performance of the AGS.
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| 7. |
- Ekholm, Jennifer, 1992, et al.
(författare)
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Microbiome structure and function in parallel full-scale aerobic granular sludge and activated sludge processes
- 2024
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Ingår i: Applied Microbiology and Biotechnology. - 1432-0614 .- 0175-7598. ; 108:1
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Tidskriftsartikel (refereegranskat)abstract
- Abstract: Aerobic granular sludge (AGS) and conventional activated sludge (CAS) are two different biological wastewater treatment processes. AGS consists of self-immobilised microorganisms that are transformed into spherical biofilms, whereas CAS has floccular sludge of lower density. In this study, we investigated the treatment performance and microbiome dynamics of two full-scale AGS reactors and a parallel CAS system at a municipal WWTP in Sweden. Both systems produced low effluent concentrations, with some fluctuations in phosphate and nitrate mainly due to variations in organic substrate availability. The microbial diversity was slightly higher in the AGS, with different dynamics in the microbiome over time. Seasonal periodicity was observed in both sludge types, with a larger shift in the CAS microbiome compared to the AGS. Groups important for reactor function, such as ammonia-oxidising bacteria (AOB), nitrite-oxidising bacteria (NOB), polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs), followed similar trends in both systems, with higher relative abundances of PAOs and GAOs in the AGS. However, microbial composition and dynamics differed between the two systems at the genus level. For instance, among PAOs, Tetrasphaera was more prevalent in the AGS, while Dechloromonas was more common in the CAS. Among NOB, Ca. Nitrotoga had a higher relative abundance in the AGS, while Nitrospira was the main nitrifier in the CAS. Furthermore, network analysis revealed the clustering of the various genera within the guilds to modules with different temporal patterns, suggesting functional redundancy in both AGS and CAS. Key points: • Microbial community succession in parallel full-scale aerobic granular sludge (AGS) and conventional activated sludge (CAS) processes. • Higher periodicity in microbial community structure in CAS compared to in AGS. • Similar functional groups between AGS and CAS but different composition and dynamics at genus level. Graphical abstract: (Figure presented.).
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| 8. |
- Maurelli, M., et al.
(författare)
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Incompressible Euler equations with stochastic forcing: A geometric approach
- 2023
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Ingår i: Stochastic Processes and Their Applications. - : Elsevier BV. - 0304-4149. ; 159, s. 101-148
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Tidskriftsartikel (refereegranskat)abstract
- We consider a stochastic version of Euler equations using the infinite-dimensional geometric approach as pioneered by Ebin and Marsden (1970). For the Euler equations on a compact manifold (possibly with smooth boundary) we establish local existence and uniqueness of a strong solution in spaces of Sobolev mappings (of high enough regularity). Our approach combines techniques from stochastic analysis and infinite-dimensional geometry and provides a novel toolbox to establish local well-posedness of stochastic non-linear partial differential equations.(c) 2023 Elsevier B.V. All rights reserved.
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| 9. |
- Modin, Klas, 1979, et al.
(författare)
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Canonical scale separation in two-dimensional incompressible hydrodynamics
- 2022
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Ingår i: Journal of Fluid Mechanics. - : Cambridge University Press (CUP). - 0022-1120 .- 1469-7645. ; 943
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Tidskriftsartikel (refereegranskat)abstract
- The rules that govern a two-dimensional inviscid incompressible fluid are simple. Yet, to characterise the long-time behaviour is a knotty problem. The fluid fulfils Euler's equations: a nonlinear Hamiltonian system with an infinite number of conservation laws. In both experiments and numerical simulations, coherent vortex structures emerge after an initial stage. These formations dominate the large-scale dynamics, but small scales also emerge and persist. The resulting scale separation resembles Kraichnan's theory of forward and backward cascades of enstrophy and energy. Previous attempts to model the double cascade use filtering techniques that enforce separation from the outset. Here, we show that Euler's equations possess an intrinsic, canonical splitting of the vorticity function. The splitting is remarkable in four ways: (i) it is defined solely by the Poisson bracket and the Hamiltonian; (ii) it characterises steady flows; (iii) it innately separates scales, enabling the dynamics behind Kraichnan's qualitative description; and (iv) it accounts for 'broken line' energy spectra observed in both experiments and numerical simulations. The splitting originates from Zeitlin's truncated model of Euler's equations in combination with a standard quantum tool: the spectral decomposition of Hermitian matrices. In addition to theoretical insight, the scale separation dynamics enables stochastic model reduction, where multiplicative noise models small scales.
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