| 1. |
- Alsanius, Beatrix, et al.
(författare)
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Produce quality and safety
- 2017
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Ingår i: Rooftop urban agriculture. - Cham : Springer International Publishing. - 9783319577197 ; , s. 195-216
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Bokkapitel (refereegranskat)
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| 2. |
- Bergstrand, Karl-Johan, et al.
(författare)
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Nulägesanalys trädgård
- 2018
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Rapport (populärvet., debatt m.m.)abstract
- Rapporten Nulägesanalys - trädgård har genomförts inom ramen för projektgruppen Insatsstyrka trädgård. Syftet med inventeringen har varit att ge svar på vem som jobbar med trädgårdsvetenskap, vilka forskargrupper och med vilka forskningsämnen, samt identifiera framtida arbetsfält inom ämnet trädgårdsvetenskap och därmed ge uppslag för framtida utveckling av forskningsfält, utbildning och samverkan. I detta ingår att kartlägga trädgårdsvetenskaplig forskning, utbildning och samverkan vid SLU. Nulägesanalysen förväntas därmed ge en grund för fortsatt strategiskt arbete med att utveckla ämnet trädgårdsvetenskap (Horticultural Science) utifrån inventerade resurser och förutsättningar.
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| 3. |
- Darlison, Julia, et al.
(författare)
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Leaf mineral content govern microbial community structure in the phyllosphere of spinach (Spinacia oleracea) and rocket (Diplotaxis tenuifolia)
- 2019
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Ingår i: Science of the Total Environment. - : Elsevier BV. - 1879-1026 .- 0048-9697. ; 675, s. 501-512
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Tidskriftsartikel (refereegranskat)abstract
- The plant microbiome is an important factor for plant health and productivity. While the impact of nitrogen (N) availability for plant growth and development is well established, its influence on the microbial phyllosphere community structure is unknown. We hypothesize that nitrogen impacts the growth and abundance of several microorganisms on the leaf surface. The bacterial and fungal communities of baby leaf spinach (Spinacia oleracea), and rocket (Diplotaxis tenuifolia) were investigated in a field trial for two years in a commercial setting. Nitrogen fertilizer was tested in four doses (basic nitrogen, basic + suboptimal, basic + commercial, basic + excess) with six replicates in each. Culture-independent (Illumina sequencing) and culture-dependent (viable count and identification of bacterial isolates) community studies were combined with monitoring of plant physiology and site weather conditions. This study found that alpha diversity of bacterial communities decreased in response to increasing nitrogen fertilizer dose, whereas viable counts showed no differences. Correspondingly, fungal communities of the spinach phyllosphere showed a decreasing pattern, whereas the decreasing diversity of fungal communities of rocket was not significant. Plant species and effects of annual variations on microbiome structure were observed for bacterial and fungal communities on both spinach and rocket. This study provides novel insights on the impact of nitrogen fertilizer regime on a nutrient scarce habitat, the phyllosphere.
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| 4. |
- Darlison, Julia, et al.
(författare)
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Plant species affects establishment of Escherichia coli O157:H7 gfp+ on leafy vegetables
- 2019
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Ingår i: Journal of Applied Microbiology. - : Oxford University Press (OUP). - 1364-5072 .- 1365-2672. ; 127, s. 292-305
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Tidskriftsartikel (refereegranskat)abstract
- Aims Greenhouse trials were conducted with different cultivars of baby leaf spinach, rocket and Swiss chard and inoculation of Escherichia coli O157:H7 gfp+, to determine whether plant species and cultivar have an impact on the establishment of this strain. Methods and Results Three cultivars each of spinach, rocket and Swiss chard were spray inoculated with E. coli O157:H7 gfp+ at doses of log 7 CFU per ml. Due to the different lengths of growing period spinach and Swiss chard were spray inoculated three times and rocket five times, with final inoculation performed 3 days prior to harvest. After a growing period of 26-33 days, E. coli O157:H7 gfp+ was recovered from the leaf surface in mean populations between log 1 and 6 CFU per gram. The lowest occurrence of E. coli O157:H7 gfp+ was found on rocket leaves and the highest on spinach. There was no significant difference in the establishment of E. coli O157:H7 gfp+ between cultivars, but there were differences between plant species. Indigenous phyllosphere bacteria were pure cultured and identified with 16S rRNA gene sequencing. Conclusions Despite the same high inoculation dose of E. coli O157:H7 gfp+ on leaves, the establishment rate differed between plant species. However, plant cultivar did not affect establishment. Pantoea agglomerans dominated the identified bacterial isolates. Significance and Impact of the Study As previous studies are inconclusive on choice of model plant species and cultivar, we studied whether plant species or cultivar determines the fate of E. coli O157:H7 gfp+ on leafy vegetables. The findings indicate that plant species is a key determinant in the establishment of E. coli O157:H7 gfp+.
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| 5. |
- Grudén, Maria, et al.
(författare)
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Processing of green leaf produce: microorganisms associated with process water and produce
- 2016
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Ingår i: Acta Horticulturae. - 0567-7572 .- 2406-6168. ; 1141, s. 373-379
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Konferensbidrag (refereegranskat)abstract
- Bacterial colonisation of a green leaf product (rocket) was studied before processing and after packing and raw and process water quality at different stages during processing under commercial conditions was analysed. “Raw water” denotes fresh water of potable water quality used to fill the washing line, whereas “process water” describes water sampled from the prewash and the main wash unit in the washing line. Samples were collected on four events, with three replicates per event. Leaf and water samples were both analysed for heterotrophic plate counts (HPC) at 22 °C, slow-growing bacteria (SGB), total coliform bacteria (TC), Escherichia coli (EC), intestinal enterococci (IE), Listeria monocytogenes (LM), Salmonella spp. and Campylobacter spp. The bacterial load in the process water increased substantially after produce came in contact with raw water. HPC exceeded 300,000 CFU mL-1 and SGB 50,000 CFU mL-1. IE were also very abundant, but not TC and EC. The microbial reduction rate was log 0.5. From these specimens, microorganisms were grown on semi-selective media (0.1x Tryptic Soy Agar, King agar B, Enterococci agar and Violet Red Bile Dextrose Agar) and five randomly selected colonies were identified using Biolog GenIII panels. Various Pseudomonas species and Pantoea agglomerans were frequent in the process water. The phyllosphere microbiota before washing was also dominated by P. agglomerans. After washing, P. agglomerans, Rahnella aquatilis and Pseudomonas spp. were abundant. Pseudomonas fluorescens and Enterobacter cloacae resisted washing procedures in the washing line and under laboratory conditions.
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| 6. |
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| 7. |
- Hartmann, Rahel, et al.
(författare)
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Impact of the source of organic manure on persistence of E. coli O157:H7 gfp+ in rocket (Diplotaxis tenuifolia) and Swiss chard (Beta vulgaris cicla)
- 2017
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Ingår i: Food Control. - : Elsevier BV. - 0956-7135 .- 1873-7129. ; 81, s. 200-210
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Tidskriftsartikel (refereegranskat)abstract
- The influence of organic nitrogen sources on the establishment of E. coil 0157:H7 and the occurrence of other potentially human pathogenic bacteria on baby leaf salads was evaluated. Greenhouse-grown rocket and Swiss chard were spray-inoculated with gfp-tagged E. coil 0157:H7 twice a week from when their first true leaves reached a length of 2 cm until three days before harvest. Analysis of nitrogen content in leaves revealed differences between treatments. Untreated plants had the lowest values, followed by plants fertilized with pig hair pellets and chicken manure. The same pattern was seen for the growth medium at the day of harvest. The applied strain showed similar establishment (measured using culture-dependent methods) irrespective of treatment, but Swiss chard hosted significantly more E. coli 0157:H7 than rocket. Differences in the risk of infection were found for the crops, with plants fertilized with pig hair pellets showing a slightly higher risk. No relationship was found for total nitrogen content in leaves and colonization with E. coli 0157:H7 gfp+. Isolating dominant Enterobacteriaceae from leaves, some strains showed conformity to bacterial species that have previously been identified to influence the establishment of E. coli 0157:H7 in the phyllosphere. In this study, no inhibitory effects were observed in vitro.Colonies showing matching characteristics for Listeria spp. and Salmonella spp. were detected up to 5.5 and 5 log CFU g(-1) fresh weight, respectively. However, presumptive Listeria spp. and Salmonella spp. could not be confirmed by sequencing of the 16S rRNA gene. (C) 2017 Elsevier Ltd. All rights reserved.
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| 8. |
- Hitimana, Regis, et al.
(författare)
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Incremental cost and health gains of the 2016 WHO antenatal care recommendations for Rwanda: results from expert elicitation
- 2019
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Ingår i: Health Research Policy and Systems. - : Springer Science and Business Media LLC. - 1478-4505. ; 17
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: High-quality evidence of effectiveness and cost-effectiveness is rarely available and relevant for health policy decisions in low-resource settings. In such situations, innovative approaches are needed to generate locally relevant evidence. This study aims to inform decision-making on antenatal care (ANC) recommendations in Rwanda by estimating the incremental cost-effectiveness of the recent (2016) WHO antenatal care recommendations compared to current practice in Rwanda. Methods: Two health outcome scenarios (optimistic, pessimistic) in terms of expected maternal and perinatal mortality reduction were constructed using expert elicitation with gynaecologists/obstetricians currently practicing in Rwanda. Three costing scenarios were constructed from the societal perspective over a 1-year period. The two main inputs to the cost analyses were a Monte Carlo simulation of the distribution of ANC attendance for a hypothetical cohort of 373,679 women and unit cost estimation of the new recommendations using data from a recent primary costing study of current ANC practice in Rwanda. Results were reported in 2015 USD and compared with the 2015 Rwandan per-capita gross domestic product (US$ 697). Results: Incremental health gains were estimated as 162,509 life-years saved (LYS) in the optimistic scenario and 65,366 LYS in the pessimistic scenario. Incremental cost ranged between $5.8 and $11 million (an increase of 42% and 79%, respectively, compared to current practice) across the costing scenarios. In the optimistic outcome scenario, incremental cost per LYS ranged between $36 (for low ANC attendance) and $67 (high ANC attendance), while in the pessimistic outcome scenario, it ranged between $90 (low ANC attendance) and $168 (high ANC attendance) per LYS. Incremental cost effectiveness was below the GDP-based thresholds in all six scenarios. Discussion: Implementing the new WHO ANC recommendations in Rwanda would likely be very cost-effective; however, the additional resource requirements are substantial. This study demonstrates how expert elicitation combined with other data can provide an affordable source of locally relevant evidence for health policy decisions in low-resource settings.
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| 9. |
- Mogren, Lars
(författare)
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Assessment of the vitamin C content in selected baby leafy species over the season
- 2018
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Ingår i: Acta Horticulturae. - 0567-7572 .- 2406-6168. ; 1209, s. 27-33
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Konferensbidrag (refereegranskat)abstract
- Baby leaf vegetables have naturally high levels of vitamin C, of which the major part is found as ascorbic acid (AsA). A higher level of AsA at harvest may prolong the shelf life of this perishable vegetable commodity. Temperature has been shown to be one of the factors that affect AsA synthesis in plants, as well as light and day length, and all these factors vary over the season. This study compared different baby leaf species, different baby leaf spinach cultivars and variation in vitamin C content in commercially grown baby leaf spinach over the season. AsA levels as well as dehydro ascorbic acid (DHA) levels on both fresh and dry weight basis were analyzed. These preliminary results indicate that vitamin C content varies greatly between baby leaf species and that exactly the same type of bag of baby leaf spinach from the same super market can contain leaves with a vitamin C content varying between 50-350 mg kg(-1) fw over the season.
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| 10. |
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