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- Li, Chunmei, et al.
(författare)
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An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes
- 2008
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Ingår i: PLOS Genetics. - : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 4:3, s. e1000044-
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Tidskriftsartikel (refereegranskat)abstract
- MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 ( Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains unknown. Here we demonstrate that the C. elegans MIP-T3 ortholog DYF-11 is an intraflagellar transport (IFT) protein that plays a critical role in assembling functional kinesin motor-IFT particle complexes. We have cloned a loss of function dyf-11 mutant in which several key components of the IFT machinery, including Kinesin-II, as well as IFT subcomplex A and B proteins, fail to enter ciliary axonemes and/or mislocalize, resulting in compromised ciliary structures and sensory functions, and abnormal lipid accumulation. Analyses in different mutant backgrounds further suggest that DYF-11 functions as a novel component of IFT subcomplex B. Consistent with an evolutionarily conserved cilia-associated role, mammalian MIP-T3 localizes to basal bodies and cilia, and zebrafish mipt3 functions synergistically with the Bardet-Biedl syndrome protein Bbs4 to ensure proper gastrulation, a key cilium- and basal body-dependent developmental process. Our findings therefore implicate MIP-T3 in a previously unknown but critical role in cilium biogenesis and further highlight the emerging role of this organelle in vertebrate development.
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- Shaw, D. A., et al.
(författare)
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A study of vibronic coupling in excited neutral and ionic states of carbon dioxide
- 2006
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Ingår i: Chemical Physics. - : Elsevier BV. - 0301-0104. ; 324:2-3, s. 515-526
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Tidskriftsartikel (refereegranskat)abstract
- Dispersed fluorescence spectroscopy and photoelectron spectroscopy have been employed to study vibronic coupling in excited neutral and ionic states of carbon dioxide. The dispersed fluorescence excitation spectra of the CO2+ B~(001) 2Sigmag+ -> X~ (001) 2Piu and the B~ (002) 2Sigmau+ -> X~ (002) 2Pig transitions have revealed new structure. Some of this structure can be assigned to nsigmag or nd Rydberg series converging onto the CO2+ C~(001) 2Sigmau+ threshold or to an np Rydberg series converging onto the CO2+ C~(002) 2Sigmag+ threshold. Autoionisation from Rydberg states belonging to these series results in the formation of ions with one or two quanta of the asymmetric stretching mode being excited. The HeI excited photoelectron spectra of the CO2+ B~ 2Sigmau+ and C~ 2Sigmag+ states reveal extensive and complex vibrational structure. Transitions involving the non-totally symmetric bending and asymmetric stretching modes have been observed. The occurrence of these normally forbidden transitions is attributed to vibronic interactions.
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