| 1. |
- Ankerst, J., et al.
(författare)
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Decreased responsiveness to immune complexes of granulocytes from patients with acute leukemia in remission demonstrated by microcalorimetry
- 1984
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Ingår i: Leukemia Research. - : Elsevier BV. - 0145-2126. ; 8:6, s. 997-1002
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Tidskriftsartikel (refereegranskat)abstract
- Functional activity of granulocytes from healthy individuals and from patients with acute leukemia in remission was studied. The increase of heat production rate (metabolic activity) after stimulation of the blood cells with in vitro formed immune complexes was measured by microcalorimeters of heat conduction type. It was demonstrated that increased heat production rate after exposure to immune complexes was significantly lower (p<0.0005) in 9 patients with acute leukemia with a remission duration of less than 6 months than in 25 healthy volunteers.
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| 2. |
- Faldt, R., et al.
(författare)
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Heat production in different populations of human blood cells exposed to immune complexes in vitro : The importance of the Fc parts of immunoglobulins and the influence of active complement
- 1982
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Ingår i: Immunology. - 0019-2805. ; 46:1, s. 189-198
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Tidskriftsartikel (refereegranskat)abstract
- By use of a batch microcalorimeter of the thermopile type, heat production was measured in isolated populations of human peripheral blood cells exposed to defined immune complexes formed in vitro. It was found that most of the heat production recorded in whole blood after admixture of immune complexes occurs in the granulocytes. Under these conditions small but constantly higher activation values were found in the absence of active complement. It was shown that complexes consisting of antigen and F(ab)2 fragments prepared from the specific antibodies were able to initiate heat production in the cells only in the presence of active complement. These experiments indicate that immune complexes are able to induce increased heat production in the cells either by binding to Fc receptors or by activation of complement through the alternative pathway and subsequent binding of the generated C3b to C3b receptors on the heat-producing cells.
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| 3. |
- Faldt, R., et al.
(författare)
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Heat production rate in polymorphonuclear granulocytes from patients with acute myelogenous leukaemia and healthy individuals
- 1984
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Ingår i: British Journal of Haematology. - : Wiley. - 0007-1048 .- 1365-2141. ; 58:4, s. 671-678
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Tidskriftsartikel (refereegranskat)abstract
- Heat production rate in polymorphonuclear granulocytes (PMN) from 18 AML patients and 21 healthy individuals was measured using microcalorimetry. An increased PMN heat production rate was found in most AML patients at diagnosis and during the first 6 months of remission. In 7/7 patients showing values above the normal mean level the heat production rate decreased during sequential analysis. The increased PMN heat production rate is serum dependent and leukaemic sera induced increased calorimetric values also in PMN from healthy donors. This effect was not due to heat labile complement factors.
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| 4. |
- Monti, M., et al.
(författare)
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A new approach to detection of antigen-antibody complexes by microcalorimetric measurements of heat production in blood cells
- 1980
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 37:1, s. 29-37
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Tidskriftsartikel (refereegranskat)abstract
- Using batch microcalorimeters of the thermopile conduction type, heat production was measured in human blood in the presence of defined antigens and their specific antibodies. A large increase in heat production, reflecting increased metabolic activity in the blood cells, was found when antigens and specific antisera at certain antigen-antibody proportions were mixed with the blood. The reproducibility and specificity of the method were found to be very good. The present results indicate that calorimetry may be a useful technique for detection of antigen-antibody complexes.
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