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Diagnostic metaboli...
Diagnostic metabolite biomarkers of chronic typhoid carriage
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- Näsström, Elin (författare)
- Umeå universitet,Kemiska institutionen
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- Jonsson, Pär (författare)
- Umeå universitet,Kemiska institutionen
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- Johansson, Anders (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)
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Dongol, Sabina (författare)
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Karkey, Abhilasha (författare)
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Basnyat, Buddha (författare)
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Thieu, Nga Tran Vu (författare)
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Van, Tan Trinh (författare)
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Thwaites, Guy E. (författare)
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- Antti, Henrik (författare)
- Umeå universitet,Kemiska institutionen
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Baker, Stephen (författare)
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(creator_code:org_t)
- 2018-01-26
- 2018
- Engelska.
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Ingår i: PLoS Neglected Tropical Diseases. - : Public Library of Science (PLoS). - 1935-2727 .- 1935-2735. ; 12:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Background: Salmonella Typhi and Salmonella Paratyphi A are the agents of enteric (typhoid) fever; both can establish chronic carriage in the gallbladder. Chronic Salmonella carriers are typically asymptomatic, intermittently shedding bacteria in the feces, and contributing to disease transmission. Detecting chronic carriers is of public health relevance in areas where enteric fever is endemic, but there are no routinely used methods for prospectively identifying those carrying Salmonella in their gallbladder.Methodology/Principal findings: Here we aimed to identify biomarkers of Salmonella carriage using metabolite profiling. We performed metabolite profiling on plasma from Nepali patients undergoing cholecystectomy with confirmed S. Typhi or S. Paratyphi A gallbladder carriage (and non-carriage controls) using two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS) and supervised pattern recognition modeling. We were able to significantly discriminate Salmonella carriage samples from non-carriage control samples. We were also able to detect differential signatures between S. Typhi and S. Paratyphi A carriers. We additionally compared carriage metabolite profiles with profiles generated during acute infection; these data revealed substantial heterogeneity between metabolites associated with acute enteric fever and chronic carriage. Lastly, we found that Salmonella carriers could be significantly distinguished from non-carriage controls using only five metabolites, indicating the potential of these metabolites as diagnostic markers for detecting chronic Salmonella carriers.Conclusions/Significance: Our novel approach has highlighted the potential of using metabolomics to search for diagnostic markers of chronic Salmonella carriage. We suggest further epidemiological investigations of these potential biomarkers in alternative endemic enteric fever settings.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
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Näsström, Elin
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Jonsson, Pär
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Johansson, Ander ...
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Dongol, Sabina
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Karkey, Abhilash ...
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Basnyat, Buddha
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visa fler...
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Thieu, Nga Tran ...
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Van, Tan Trinh
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Thwaites, Guy E.
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Antti, Henrik
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Baker, Stephen
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visa färre...
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Umeå universitet