Optimization of the detection of microbes in blood from immunocompromised patients with haematological malignancies.

• The present study aimed to improve the rate of detection of blood-borne microbes by using PCRs with pan-bacterial and Candida specificity. Seventeen per cent of the blood samples (n=178) collected from 107 febrile patients with haematological malignancies were positive using standard culture (BacT/Alert system). Candida PCR was positive in 12 patients, only one of whom scored culture-positive. Bacterial PCR using fresh blood samples was often negative, but the detection rate increased when the blood was pre-incubated for 2 days. These data indicate that PCR assays might be a complement for the detection of blood-borne opportunists in immunocompromised haematology patients.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

Nyckelord

Bacteremia

epidemiology

microbiology

Blood

microbiology

Candida

classification

genetics

isolation & purification

Candida albicans

genetics

isolation & purification

Candida glabrata

genetics

isolation & purification

Candidiasis

microbiology

Culture Media

DNA

Bacterial

analysis

DNA

Fungal

analysis

Fever

Fungemia

epidemiology

microbiology

Gram-Negative Bacteria

genetics

isolation & purification

Gram-Positive Bacteria

genetics

isolation & purification

Hematologic Neoplasms

complications

Humans

Immunocompromised Host

Neutropenia

Opportunistic Infections

epidemiology

microbiology

Polymerase Chain Reaction

methods

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)