| 1. |
- Aoyama, K., et al.
(författare)
-
Cleavage of integrin by mu-calpain during hypoxia in human endometrial cells
- 2004
-
Ingår i: Am J Reprod Immunol. - 1046-7408. ; 52:6, s. 362-9
-
Tidskriftsartikel (refereegranskat)abstract
- PROBLEM: The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated. METHOD OF STUDY: Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis. RESULTS: The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia. CONCLUSIONS: Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium.
|
|
| 2. |
- Fujita, Y, et al.
(författare)
-
Evidence for the existence of the [202]3/2 deformed band in mirror nuclei Mg-25 and Al-25
- 2004
-
Ingår i: Physical Review Letters. - 1079-7114. ; 92:6
-
Tidskriftsartikel (refereegranskat)abstract
- After 50 years of its prediction, the highest-lying [2 0 2]3/2 orbit among the six Nilsson single-particle orbits originating from the sd shells in prolately deformed nuclei and the rotational band on this orbit were identified. The band members were observed in Al-25 at excitation energies of 6-7.5 MeV in a high-resolution Mg-25(He-3,t) charge-exchange reaction at 0degrees having a strong selectivity for Gamow-Teller transitions. In the comparison with the analogous M1 transitions in Mg-25, the J(pi)=3/2(+) bandhead state and the excited 5/2(+) and 7/2(+) members were clearly assigned.
|
|
| 3. |
- Shimbara, Y, et al.
(författare)
-
Suppression of Gamow-Teller and M1 transitions in deformed mirror nuclei Mg-25 and Al-25 - Direct observation of K selection rules
- 2004
-
Ingår i: European Physical Journal A. Hadrons and Nuclei. - : Springer Science and Business Media LLC. - 1434-6001. ; 19:1, s. 25-31
-
Tidskriftsartikel (refereegranskat)abstract
- The mirror nuclei Mg-25 and Al-25 are expected to have very similar structures. The Gamow-Teller (GT) transitions from the J(pi) = 5/2(+) ground state of Mg-25 to the excited states in Al-25 were studied by high-resolution measurements of the Mg-25(He-3,t) charge-exchange reaction at 0degrees and at 140 MeV/nucleon. Assuming the usual DeltaJ(pi) = 1(+) selection rule for the spin-isospin-type GT transitions, the states with J(pi) = 3/2(+), 5/2(+), and 7/2 (+) should be excited. However, of the more than ten states with these J(pi) values below 6 MeV excitation energy, only the 5/2 (+) ground state and the 7/2 (+) , 1.613 MeV state in Al-25 were strongly populated, while all other states were strongly suppressed. The analysis of M1 transitions in Mg-25 also suggested a very similar feature for the analogous M1 transitions. Both Mg-25 and Al-25 are known to be largely deformed, and most low-lying states can be interpreted in terms of one-particle quantum numbers in the deformed potential and the associated rotational spectra. The observed suppression can be explained in terms of the K quantum number selection rules that are inherent to axially deformed nuclei.
|
|
| 4. |
- Nishikawa, H., et al.
(författare)
-
The role of cathepsin B and cystatin C in the mechanisms of invasion by ovarian cancer
- 2004
-
Ingår i: Gynecol Oncol. - : Elsevier BV. - 0090-8258. ; 92:3, s. 881-6
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The aim of this study was to investigate the contribution of cathepsin B and cystatin C to the mechanisms of invasion by ovarian cancer. MATERIALS AND METHODS: Using surgical materials from patients with ovarian cancer, immunohistochemistry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analysis were performed using antibodies against cathepsin B or cystatin C. Serum levels of cathepsin B and cystatin C in patients with benign and malignant ovarian lesions were determined by enzyme-linked immunosorbent assay (ELISA). An invasion assay using an ovarian cancer cell line was performed by addition of cystatin C or specific inhibitors of cathepsin B. RESULTS: While immunohistochemical staining of cathepsin B and cystatin C was evident in cancer cells and associated stromal tissue, this was not the case in benign tumors. The malignancies were also found to be positive for cathepsin B and cystatin C by SDS-PAGE and Western blotting analysis. No significant difference in serum cathepsin B levels was observed between patients with benign and malignant disease. However, the concentration of cystatin C in cases with ovarian cancer was significantly higher in benign cases (P<0.0001) and in healthy controls (P<0.0001). Invasion by cancer cells was dose-dependently suppressed by cystatin C and cathepsin B inhibitors. CONCLUSION: The results provided convincing evidence that cathepsin B and cystatin C may contribute to the mechanisms of invasion of ovarian cancer.
|
|
