| 1. |
- Edrén, Johannes, et al.
(författare)
-
Modelica and Dymola for education in vehicle dynamics at KTH
- 2009
-
Ingår i: Proceedings from 7th Modelica Conference 2009. - : Linköping University Electronic Press. ; , s. 775-783
-
Konferensbidrag (refereegranskat)abstract
- Dymola and Modelica has been used at KTH Vehicle Dynamics (KTHVD) for research work since 2000, see e.g. [1]. With the Vehicle Dynamics Library (VDL) [2], Modelica has become far more accessible for both researchers and students in the field of vehicle dynamics. Therefore a project aiming at introducing it as a tool in education was initiated in order to evaluate the current state of Dymola and Modelica as tools for wider use in education at the division. The work presented in this paper was realized as a part of a PhD course, where one of the tasks were to design dedicated exercises to illustrate fundamentals of vehicle dynamics for students.
|
|
| 2. |
- Göransson, Jenny, 1978-, et al.
(författare)
-
A single molecule array for digital targeted molecular analyses
- 2009
-
Ingår i: Nucleic Acids Research. - England : Oxford University Press. - 0305-1048 .- 1362-4962. ; 37:1, s. e7-
-
Tidskriftsartikel (refereegranskat)abstract
- We present a new random array format together with a decoding scheme for targeted multiplex digital molecular analyses. DNA samples are analyzed using multiplex sets of padlock or selector probes that create circular DNA molecules upon target recognition. The circularized DNA molecules are amplified through rolling-circle amplification (RCA) to generate amplified single molecules (ASMs). A random array is generated by immobilizing all ASMs on a microscopy glass slide. The ASMs are identified and counted through serial hybridizations of small sets of tag probes, according to a combinatorial decoding scheme. We show that random array format permits at least 10 iterations of hybridization, imaging and dehybridization, a process required for the combinatorial decoding scheme. We further investigated the quantitative dynamic range and precision of the random array format. Finally, as a demonstration, the decoding scheme was applied for multiplex quantitative analysis of genomic loci in samples having verified copy-number variations. Of 31 analyzed loci, all but one were correctly identified and responded according to the known copy-number variations. The decoding strategy is generic in that the target can be any biomolecule which has been encoded into a DNA circle via a molecular probing reaction.
|
|
| 3. |
- Rönn, Ann-Charlotte, 1969-
(författare)
-
Analysis of Nucleotide Variations in Non-human Primates
- 2007
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Many of our closest relatives, the primates, are endangered and could be extinct in a near future. To increase the knowledge of non-human primate genomes, and at the same time acquire information on our own genomic evolution, studies using high-throughput technologies are applied, which raises the demand for large amounts of high quality DNA.In study I and II, we evaluated the multiple displacement amplification (MDA) technique, a whole genome amplification method, on a wide range of DNA sources, such as blood, hair and semen, by comparing MDA products to genomic DNA as templates for several commonly used genotyping methods. In general, the genotyping success rate from the MDA products was in concordance with the genomic DNA. The quality of sequences of the mitochondrial control region obtained from MDA products from blood and non-invasively collected semen samples was maintained. However, the readable sequence length was shorter for MDA products.Few studies have focused on the genetic variation in the nuclear genes of non-human primates. In study III, we discovered 23 new single nucleotide polymorphisms (SNPs) in the Y-chromosome of the chimpanzee. We designed a tag-microarray minisequencing assay for genotyping the SNPs together with 19 SNPs from the literature and 45 SNPs in the mitochondrial DNA. Using the microarray, we were able to analyze the population structure of wild-living chimpanzees.In study IV, we established 111 diagnostic nucleotide positions for primate genera determination. We used sequence alignments of the nuclear epsilon globin gene and apolipoprotein B gene to identify positions for determination on the infraorder and Catarrhini subfamily level, respectively, and sequence alignments of the mitochondrial 12S rRNA (MT-RNR1) to identify positions to distinguish between genera. We designed a microarray assay for immobilized minisequencing primers for genotyping these positions to aid in the forensic determination of an unknown sample.
|
|
| 4. |
- Sunnegårdh-Grönberg, Karin, 1969-, et al.
(författare)
-
Selection of dental materials and longevity of replaced restorations in Public Dental Health clinics in northern Sweden.
- 2009
-
Ingår i: Journal of Dentistry. - : Elsevier BV. - 0300-5712 .- 1879-176X. ; :37, s. 673-678
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVES: To investigate the selection of direct restorative materials and longevity of replaced restorations in relation to operator and patients characteristics. METHODS: A cross-sectional study of treatment in practice, recording all new placements and replacements of direct restorations was performed during 2 weeks comprising all dentists within the Public Dental Health clinics in the county council of Västerbotten. RESULTS: A total of 2834 data collection sheets, one for each placed restoration, were received with a dropout of 10%. Restorations analyzed in the study were placed in permanent teeth in patients older than 15 years. First restorations placed due to primary caries were 671 and replacements 1536. Class II was the most frequently treated cavity followed by class I. The median longevity of replaced restorations was for amalgam, resin based composite and glass ionomer 16, 6 and 11 years, respectively. High caries risk patients showed shorter longevity for resin based composite restorations than low or moderate risk patients. Secondary caries as reason for failure for class II resin based composite restorations occurred significantly later than loss or fracture. Significantly longer longevity was observed for replaced restorations executed by more experienced dentists. CONCLUSIONS: The use of amalgam was negligible and the material was predominantly replaced by resin based composites in first and replaced restorations. Class II was the most frequent placed and replaced restorations. Caries risk and experience of operator influenced longevity of replacements.
|
|
