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Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules

Nyberg, Lena, 1979 (författare)
Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Quaderi, Mahmood Saair, 1990 (författare)
Chalmers University of Technology,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden / Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.;Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.
Emilsson, Gustav, 1989 (författare)
Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.;Chalmers, Dept Appl Phys, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden / Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden
visa fler...
Karami, N. (författare)
University of Gothenburg,Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden.,Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Lagerstedt, Erik (författare)
Lunds universitet,Lund University,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.
Müller, Vilhelm, 1990 (författare)
Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Noble, Charleston (författare)
Lund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden
Hammarberg, S. (författare)
Lunds universitet,Lund University,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.
Nilsson, Adam N. (författare)
Lund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden
Sjoberg, F. (författare)
University of Gothenburg,Univ Gothenburg, Sahlgrenska Acad, Dept Infect Dis, Gothenburg, Sweden.,Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
Fritzsche, Joachim, 1977 (författare)
Chalmers University of Technology,Chalmers, Dept Appl Phys, Gothenburg, Sweden.,Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden
Sandegren, Linus (författare)
Uppsala universitet,Uppsala University,Institutionen för medicinsk biokemi och mikrobiologi,Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
Ambjörnsson, Tobias (författare)
Lund University,Lunds universitet,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science,Lund Univ, Dept Astron & Theoret Phys, Lund, Sweden.,Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden
Westerlund, Fredrik, 1978 (författare)
Chalmers University of Technology,Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden.,Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden
Kristiansson, Erik, 1978 (författare)
University of Gothenburg,Chalmers University of Technology,Univ Gothenburg, Dept Math Sci, Chalmers Univ Technol, Gothenburg, Sweden.,Department of Mathematical Sciences, Chalmers University of Technology, University of Gothenburg, Gothenburg, Sweden
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Chalmers University of Technology Chalmers, Dept Biol & Biol Engn, Gothenburg, Sweden (creator_code:org_t)
2016-07-27
2016
Engelska.
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322 .- 2045-2322. ; 6
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)
NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
NATURVETENSKAP  -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Other Physics Topics (hsv//eng)
NATURVETENSKAP  -- Data- och informationsvetenskap -- Bioinformatik (hsv//swe)
NATURAL SCIENCES  -- Computer and Information Sciences -- Bioinformatics (hsv//eng)

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