| 1. |
- Al-Smadi, Derar, 1983-, et al.
(författare)
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Chemical and Biochemical Approaches for the Synthesis of Substituted Dihydroxybutanones and Di-, and Tri-Hydroxypentanones
- 2019
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Ingår i: Journal of Organic Chemistry. - : American Chemical Society (ACS). - 0022-3263 .- 1520-6904. ; 84:11, s. 6982-6991
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Tidskriftsartikel (refereegranskat)abstract
- Polyhydroxylated compounds are building blocks for the synthesis of carbohydrates and other natural products. Their synthesis is mainly achieved by different synthetic versions of aldol-coupling reactions, catalyzed either by organocatalysts, enzymes or metal-organic catalysts. We have investigated the formation of 1,4-substituted 2,3-dihydroxybutan-1-one derivatives from para- and meta-substituted phenylacetaldehydes by three distinctly different strategies. The first involved a direct aldol reaction with hydroxyacetone, dihydroxyacetone or 2-hydroxyacetophenone, catalyzed by the cinchona derivative cinchonine. The second was reductive cross-coupling with methyl or phenyl glyoxal promoted by SmI2 resulting in either 5-substituted 3,4-dihydroxypentan-2-ones or 1,4 bis-phenyl substituted butanones, respectively. Finally, in the third case, aldolase catalysis was employed for synthesis of the corresponding 1,3,4-trihydroxylated pentan-2-one derivatives. The organocatalytic route with cinchonine generated distereomerically enriched syn products (de = 60−99 %), with moderate enantiomeric excesses (ee = 43−56%), but did not produce aldols with either hydroxyacetone or dihydroxyacetone as donor ketones. The SmI2-promoted reductive cross-coupling generated product mixtures with diastereomeric and enantiomeric ratios close to unity. This route allowed for the production of both 1-methyl- and 1-phenylsubstituted 2,3-dihydroxybutanones, at yields between 40−60%. Finally, the biocatalytic approach resulted in enantiopure syn (3R,4S) 1,3,4-trihydroxypentan-2-ones.
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| 2. |
- Al-Smadi, Derar, 1983-, et al.
(författare)
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Synthesis of substrates for aldolase-catalyzed reactions : A comparison of methods for the synthesis of substituted phenylacetaldehydes
- 2018
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Ingår i: Synlett. - : Georg Thieme Verlag KG. - 0936-5214 .- 1437-2096. ; 29:9, s. 1187-1190
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Tidskriftsartikel (refereegranskat)abstract
- Methods for the synthesis of phenylacetaldehydes (oxidation, one-carbon chain extension) were compared by using the synthesis of 4-methoxyphenylacetaldehyde as a model example. Oxidations of 4-methoxyphenylethanol with activated DMSO (Swern oxidation) or manganese dioxide gave unsatisfactory results; whereas oxidation with 2-iodoxybenzoic add (IBX) produced 4-methoxyphenylacetaldehyde in reasonable (75%) yield. However, Wittig-type one-carbon chain extension with methoxymethylene-triphenylphosphine followed by hydrolysis gave an excellent (81% overall) yield of 4-methoxyphenylacetaldehyde from 4-methoxybenzaldehyde (a cheap starting material). This approach was subsequently used to synthesise a set of 10 substituted phenylacetaldehydes in good to excellent yields.
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| 3. |
- Allard, Christina, et al.
(författare)
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Rasbiologiskt språkbruk i statens rättsprocess mot sameby : DN Debatt 2015-06-11
- 2015
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Annan publikation (populärvet., debatt m.m.)abstract
- Statens hantering av forskningsresultat i rättsprocessen med Girjas sameby utgör ett hot mot Sverige som rättsstat och kunskapsnation. Åratal av svensk och internationell forskning underkänns och man använder ett språkbruk som skulle kunna vara hämtat från rasbiologins tid. Nu måste staten ta sitt ansvar och börja agera som en demokratisk rättsstat, skriver 59 forskare.
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| 4. |
- Chahtane, Hicham, et al.
(författare)
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LEAFY activity is post-transcriptionally regulated by BLADE ON PETIOLE2 and CULLIN3 in Arabidopsis
- 2018
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Ingår i: New Phytologist. - : Wiley-Blackwell. - 0028-646X .- 1469-8137. ; 220:2, s. 579-592
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Tidskriftsartikel (refereegranskat)abstract
- The Arabidopsis LEAFY (LFY) transcription factor is a key regulator of floral meristem emergence and identity. LFY interacts genetically and physically with UNUSUAL FLORAL ORGANS, a substrate adaptor of CULLIN1-RING ubiquitin ligase complexes (CRL1). The functionally redundant genes BLADE ON PETIOLE1 (BOP1) and -2 (BOP2) are potential candidates to regulate LFY activity and have recently been shown to be substrate adaptors of CULLIN3 (CUL3)-RING ubiquitin ligases (CRL3). We tested the hypothesis that LFY activity is controlled by BOPs and CUL3s in plants and that LFY is a substrate for ubiquitination by BOP-containing CRL3 complexes. When constitutively expressed, LFY activity is fully dependent on BOP2 as well as on CUL3A and B to regulate target genes such as APETALA1 and to induce ectopic flower formation. We also show that LFY and BOP2 proteins interact physically and that LFY-dependent ubiquitinated species are produced invitro in a reconstituted cell-free CRL3 system in the presence of LFY, BOP2 and CUL3. This new post-translational regulation of LFY activity by CRL3 complexes makes it a unique transcription factor subjected to a positive dual regulation by both CRL1 and CRL3 complexes and suggests a novel mechanism for promoting flower development.
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| 5. |
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| 6. |
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| 7. |
- Reiman, Arto, et al.
(författare)
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Risk factors contributing to truck drivers€™ non-driving occupational accidents
- 2018
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Ingår i: International Journal of Physical Distribution & Logistics Management. - : Emerald Group Publishing Limited. - 0960-0035 .- 1758-664X. ; 48:2, s. 183-199
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Tidskriftsartikel (refereegranskat)abstract
- Purpose The purpose of this paper is to identify various individual factors and combinations thereof that can contribute to truck drivers' occupational accidents, particularly connected to work performed outside the cab in various work environments.Design/methodology/approach In total, 74 accidents were analysed through in-depth interviews with truck drivers. These interviews were conducted employing the critical incident technique, and analysed utilising a qualitative content analysis approach.Findings The contributing factors identified were categorised into 14 categories. In all, 13 of these were grouped into four sections reflecting the drivers' work outside the cab: Goods and equipment, Loading/unloading area, Loading/unloading tasks, and Organisation. A single risk factor was associated with 40 accidents while the other 34 involved combinations of factors.Research limitations/implications Although the tasks performed by truck drivers in different countries are probably similar, one limitation might be that all the accidents characterised occurred in one country: Sweden.Practical implications The findings reveal that complex combinations of risk factors often contribute to accidents. In addition to the transportation company itself, other stakeholders, such as clients, and designers and manufacturers of technology, may influence the occupational safety of truck drivers. Different stakeholders who could contribute to managerial decision making that is designed to prevent accidents are identified and discussed.Originality/value This investigation contributes to an in-depth understanding of the causes of accidents in the transportation industry. The findings are discussed from the perspective of the stakeholders and safety management in an attempt to identify key stakeholders who can improve accident prevention.
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| 8. |
- Svensson, Robert, 1982-
(författare)
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Från träningsoverall till trenchcoat : Tränarpositionens förändring inom svensk herrelitfotboll mellan 1960- och 2010-talet
- 2019
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The position of the coach in elite sports has changed radically over the last 50 years. Using the Swedish male elite soccer coach as an example, the aim of this thesis is to acquire a deeper understanding of this change. The purpose is to examine the changing position of the Swedish male elite soccer coach in relation to the changing conditions of elite soccer between the 1960s and the 2010s. The history of the Swedish soccer coach is analysed through the lens of the Foucauldian perspective of governmentality. Focusing on the constitution of subject positions, the thesis identifies the power/knowledge relationships formulated within the governmentalities of Swedish elite football clubs and the Swedish Football Association (SvFF). A text analysis of board minutes and annual reports from the Swedish elite soccer club IFK Norrköping, education material from coaching courses organised by SvFF and the transcripts of semi-structured qualitative interviews with former and still active Swedish elite soccer coaches has been conducted.The analysis shows that at the beginning of the 1960s the established power/knowledge relationship implied that, kitted out in a whole and clean tracksuit, the coach was expected to govern the players’ techniques and fitness training twice a week. However, in order to be internationally competitive, in 1967 SvFF overturned the amateur regulations and instead allowed Swedish clubs to sign professional contracts with the players. From then on the coach was given more time to “conduct the conduct” of the players. In parallel, the clubs and SvFF turned to scientific knowledge and expertise in the areas of physiology and tactics and later sport psychology, leadership and communication. As a consequence, new power/knowledge relationships were formulated which meant that the coach was not only expected to govern new and more dimensions of the players, but also a growing staff of assistant coaches. In addition, dressed in tailor-made trench coat, the coach became the club’s outward face for attracting sponsors and communicating with the media and fans.
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| 9. |
- Uusitalo, Pia, et al.
(författare)
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The salicylidene acylhydrazide INP0341 attenuates Pseudomonas aeruginosa virulence in vitro and in vivo
- 2017
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Ingår i: Journal of antibiotics (Tokyo. 1968). - : Japan Antibiotics Research Association. - 0021-8820 .- 1881-1469. ; 70:9, s. 937-943
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Tidskriftsartikel (refereegranskat)abstract
- Pseudomonas aeruginosa is an opportunistic pathogen that can be very hard to treat because of high resistance to different antibiotics and alternative treatment regimens are greatly needed. An alternative or a complement to traditional antibiotic is to inhibit virulence of the bacteria. The salicylidene acylhydrazide, INP0341, belongs to a class of compounds that has previously been shown to inhibit virulence in a number of Gram-negative bacteria. In this study, the virulence blocking effect of INP0341 on P. aeruginosa was studied in vitro and in vivo. Two important and closely related virulence system were examined, the type III secretion system (T3SS) that translocates virulence effectors into the cytosol of the host cell to evade immune defense and facilitate colonization and the flagella system, needed for motility and biofilm formation. INP0341 was shown to inhibit expression and secretion of the T3SS toxin exoenzyme S (ExoS) and to prevent bacterial motility on agar plates and biofilm formation. In addition, INP0341 showed an increased survival of P. aeruginosa-infected mice. In conclusion, INP0341 attenuates P. aeruginosa virulence.
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| 10. |
- Zhang, Bo, et al.
(författare)
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BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance
- 2017
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Ingår i: eLIFE. - : eLife Sciences Publications, Ltd. - 2050-084X. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Both light and temperature have dramatic effects on plant development. Phytochrome photoreceptors regulate plant responses to the environment in large part by controlling the abundance of PHYTOCHROME INTERACTING FACTOR (PIF) transcription factors. However, the molecular determinants of this essential signaling mechanism still remain largely unknown. Here, we present evidence that the BLADE-ON-PETIOLE (BOP) genes, which have previously been shown to control leaf and flower development in Arabidopsis, are involved in controlling the abundance of PIF4. Genetic analysis shows that BOP2 promotes photo-morphogenesis and modulates thermomorphogenesis by suppressing PIF4 activity, through a reduction in PIF4 protein level. In red-light-grown seedlings PIF4 ubiquitination was reduced in the bop2 mutant. Moreover, we found that BOP proteins physically interact with both PIF4 and CULLIN3A and that a CULLIN3-BOP2 complex ubiquitinates PIF4 in vitro. This shows that BOP proteins act as substrate adaptors in a CUL3BOP1/BOP2 E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation.
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