| 1. |
- Berger, Roland, et al.
(författare)
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C-band heteromorphism in breast cancer patients
- 1985
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Ingår i: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 18:1, s. 37-42
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Tidskriftsartikel (refereegranskat)abstract
- The pattern of heteromorphism in the C-band-positive constitutive heterochromatin of human chromosomes #1, #9, and #16 was studied in peripheral lymphocytes of 54 breast cancer patients and 78 control individuals. The parameters of the heterochromatic regions analyzed were relative size, symmetry-asymmetry within homologous pairs, and prevalence of inversions. Significant differences between the two groups were found in C-band size of chromosomes #1, #9, and #16 and in incidence of inversions on chromosomes #1 and #9. Significant differences were noted between premenopausal and postmenopausal cancer patients in regard to inversions on chromosome #9 and between familial and sporadic patients in regard to C-band size on chromosome #16.
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| 2. |
- Brandt, Lars, et al.
(författare)
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Proliferative activity and number of clonal chromosome aberrations in non‐Hodgkin's lymphomas
- 1986
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Ingår i: Scandinavian Journal of Haematology. - : Wiley. - 0036-553X. ; 37:2, s. 106-110
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Tidskriftsartikel (refereegranskat)abstract
- Thymidine labelling indices (LI) and the number of clonal chromosome aberrations were assessed in fine‐needle aspirates from enlarged lymph nodes in 22 patients with non‐Hodgkin's lymphoma (NHL). 11 patients had LI > 3.0 (median 8.4) and 11 others had LI < 3.0 (median 1.0). Three categories of chromosome aberration were recorded: normal karyotype, 1–5 aberrations, and ≥ 6 aberrations or multiple complex changes. The distribution of these categories was different among the patients with LI > 3.0 compared to those with LI < 3.0 (p = 0.02). 8 of 11 patients with LI > 3.0 had ≥ 6 or complex changes. The corresponding figures for patients with LI < 3.0 were 2 out of 11. When 16 previously untreated patients were analysed separately, the median number of clonal aberrations was 6.5 in 8 patients with LI > 3.0 and 2.5 in 8 others with LI < 3.0 (p = 0.025). The results suggest that early and spontaneous changes in the genetic material are common in lymphomas with a high proliferative activity. According to previous studies, therapeutic results are especially poor in NHL with high LI. It is proposed that a high proliferative activity of lymphoma cells facilitates an early development of several new mutants and that some of these may be resistant to chemotherapeutic agents.
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| 3. |
- Brandt, Lars, et al.
(författare)
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Relation between occupational exposure to organic solvents and chromosome aberrations in non‐Hodgkin's lymphoma
- 1989
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Ingår i: European Journal of Haematology. - : Wiley. - 0902-4441 .- 1600-0609. ; 42:3, s. 298-302
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Tidskriftsartikel (refereegranskat)abstract
- Chromosome analysis of lymphoma cells was performed in 54 untreated patients with non‐Hodgkin's lymphoma (NHL). 10 patients had a history of daily occupational handling of organic solvents for at least 1 year (exposed group) and 44 patients had never (or only for shorter periods) worked with solvents (unexposed group). There were no differences between exposed and unexposed patients regarding age, clinical stage or histologic malignancy grade. The patients were assigned to three categories: Patients with 0–4, 5–9, or ≥ 10 cytogenetic events producing clonal aberrations of the lymphoma cells. The proportions of exposed patients in these categories were 2/26 (8%), 5/20 (25%) and 3/8 (38%); respectively, i.e. with increasing numbers of events there was an increasing probability of previous exposure to solvents (p = 0.035; trend analysis). 5 of 7 exposed patients (71%) with intermediate or high‐grade lymphomas displayed translocations involving the band 14q32. Such 14q+ markers were found in only 5 out of 28 unexposed patients (18%) with lymphomas of comparable malignancy grade (p = 0.01). Among unexposed patients with intermediate or high‐grade lymphoma the most common clonal aberration was 6q‐ which occurred in 10 out of 28 patients (36%). This abnormality was not observed in the exposed patients with lymphomas of corresponding malignancy grades (p = 0.08). It thus appears that the number of clonal chromosome aberrations is especially large in NHL patients with a history of occupational exposure to organic solvents. Moreover, such exposure may be associated with characteristic cytogenetic changes in the lymphoma cells.
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| 4. |
- Brandt, Lars, et al.
(författare)
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Survival following combination chemotherapy in advanced high grade non‐Hodgkin's lymphomas : Relation to proliferative activity of the lymphoma cells
- 1987
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Ingår i: European Journal of Haematology. - : Wiley. - 0902-4441 .- 1600-0609. ; 38:5, s. 437-441
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Tidskriftsartikel (refereegranskat)abstract
- In 18 untreated adult patients (median age 62.5 yr) with advanced non‐Hodgkin's lymphoma of unfavourable histology, thymidine labelling indices (LIs) of the lymphoma cells were assessed. The patients were treated with combination chemotherapy and have been followed for 29–60 (median 52) months or until death. The survival curve had a steep fall during the first 2 yr. Between 2–5 yr after treatment there was a flattening of the curve and survival seemed to be similar to the survival expected for a Swedish population matched for age and sex. 11 patients died with 2 yr and 7 patients have survived for a longer period. Age, histopathologic classification and clinical stages were comparable in short‐term and long‐term survivors and treatment was not more aggressive for the long‐term survivors. The LIs were significantly higher (median 8.2) in short‐term survivors than in the long‐term survivors (median 1.4). Long‐term survival following combination chemotherapy of advanced NHL of unfavourable histology seems to be achieved mainly in patients with a low proliferative activity of the lymphoma cells. It is suggested that in NHL a high proliferative activity may facilitate the generation of new mutants and that some of these are spontaneously resistant to various chemotherapeutic drugs.
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| 5. |
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| 6. |
- JOHANSSON, BERTIL, et al.
(författare)
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Normal frequency of structural chromosome aberrations in fibroblasts from patients with non‐Hodgkin's lymphoma
- 1988
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 109:2, s. 277-280
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Tidskriftsartikel (refereegranskat)abstract
- The incidence of chromosome aberrations, i.e., chromatid and chromosome gaps, breaks, and exchanges, was studied in cultured skin fibroblasts from 25 untreated patients with non‐Hodgkin's lymphoma (NHL) and 26 controls. The mean frequencies of aberrant cells, and gap, break, and gap+break events per 100 metaphases were 4.2, 1.9, 2.8, and 4.7 in the NHL group, and 5.1, 2.6, 3.2, and 5.8 in the control group. None of these parameters differed significantly between the groups, indicating that constitutional chromosomal instability is not related to the development of NHL. In the total material there was a significant (P<0.05) increase with age in the number of aberrant cells.
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| 7. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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C‐band polymorphism in non‐Hodgkin lymphoma
- 1985
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 103:1, s. 85-87
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Tidskriftsartikel (refereegranskat)abstract
- The pattern of heteromorphism in C‐band positive constitutive heterochromatin of human chromosomes Nos. 1, 9, and 16 was studied in peripheral lymphocytes from 78 patients with non‐Hodgkin lymphoma and 78 control individuals. The parameters of the heterochromatic regions analyzed were relative size, symmetry‐asymmetry within homologous pairs, and prevalence of inversions. No consistent differences were found in these parameters between non‐Hodgkin lymphoma patients and controls.
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| 8. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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CYTOGENETIC STUDIES IN HODGKIN'S DISEASE
- 1987
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Ingår i: Acta Pathologica Microbiologica Scandinavica. Section A. Pathology. - 0108-0164. ; 95 A:1-6, s. 289-295
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Tidskriftsartikel (refereegranskat)abstract
- Cytogenetic analysis was attempted in 20 patients with Hodgkin's disease. No mitoses were found in 2 cases, normal metaphases in 7, and normal metaphases with nonclonal aberrations in 7. Of the 4 cases with clonal aberrations, one had +16 as the sole change, whereas the remaining tumors had multiple numerical and structural changes.
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| 9. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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Cytogenetic studies in non‐Hodgkin lymphomas ‐ Results from surgical biopsies
- 1986
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 104:1, s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- Cytogenetic analysis using Giemsa banding technique was successful in 30 of the 49 adult patients with nonHodgkin lymphomas investigated. The results are correlated with previous findings in 48 non‐Hodgkin lymphoma patients studied by means of fine needle aspiration in our laboratory. As 8 patients were included in both series, the total number successfully investigated was 70. The success rate with both sampling techniques was equivalent, and both methods also seemed to give qualitatively similar information about chromosome pattern. However, cells with a normal diploid karyotype were more frequent in the surgical biopsies. In the total material, normal karyotypes only were found in 10 patients. In two patients the aberrations were too complex to allow evaluation. The chromosome variation among the remaining 58 cases was distinctly nonrandom. Chromosomes 3, 7, 12, and 18 were preferentially gained, whereas chromosomes 1, 6, and 14 were most often involved in structural rearrangements. A 14q+ marker chromosome was the single most frequent abnormality; originating through t(14; 18) (q32; q21) in 10 of 23 cases. The second most common structural aberration was a deletion of the long arm of chromosome 6 with breakpoints at bands q15 and q21 (12 cases). At least one of the two most common numerical and/or structural changes, +7, +12, 14q+, and 6q‐, were present in 39 of the 58 patients with aberrations (67%). Longitudinal studies demonstrated karyotypic evolution during the course of the disease in five of six patients. Simultaneous samples from different tumor sites were studied in 10 patients. The findings in 9 cases suggested a monoclonal origin; in one case totally unrelated karyotypes were found in two different lymph nodes at third relapse, suggesting a multifocal origin.
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| 10. |
- KRISTOFFERSSON, ULF, et al.
(författare)
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Cytogenetic studies in non‐Hodgkin lymphomas‐Results from fineneedle aspiration samples
- 1985
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 0018-0661. ; 103:1, s. 63-76
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Tidskriftsartikel (refereegranskat)abstract
- 118 samples obtained by means of fine‐needle aspiration from 86 cases of non‐Hodgkin, non‐Burkitt lymphomas have been investigated cytogenetically with chromosome banding technique. If>5 times 106 cells were obtained at aspiration the success rate with the technique used was almost 70%, while if the number of cells was lower it was only about 15%. Technical aspects, and detailed cytogenetic findings in 48 successful cases, are reported. There was no difference in success rate due to tumor stage, or sampling at diagnosis or in relapse. It is concluded that the fine‐needle aspiration technique has certain advantages in cancer cytogenetics; it is easy to handle, requires no surgical intervention, causes little distress to the patient, and the success rate is acceptable.
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