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Träfflista för sökning "WFRF:(Oberthür Dominik) "

Search: WFRF:(Oberthür Dominik)

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1.
  • Brändén, Gisela, 1975, et al. (author)
  • Coherent diffractive imaging of microtubules using an X-ray laser.
  • 2019
  • In: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10:1
  • Journal article (peer-reviewed)abstract
    • X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.
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2.
  • Galchenkova, Marina, et al. (author)
  • Radiation damage in a hemoglobin crystal studied with an X-ray free-electron laser
  • Other publication (other academic/artistic)abstract
    • Radiation damage is a topic since the dawn of X-ray crystallography, and has gained new importance in the era of X-ray free-electron lasers (XFELs), due to their unprecedented brilliance and pulse duration. One of the driving questions has been how short the XFEL pulse has to be for the structural information to be ”damage free”. Here we compare data from Serial Femtosecond Crystallography (SFX) experiments conducted with a 3 fs and a 10 fs X-ray pulse. We conclude that even if the estimated displacement of atoms in the sample is an order of magnitude larger in the case of the 10 fs experiment, the displacement is still too small to affect the experimental data at a resolution relevant for structural determination.
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3.
  • Prasciolu, Mauro, et al. (author)
  • On the use of multilayer Laue lenses with X-ray free electron lasers
  • 2021
  • In: International Conference on X-Ray Lasers 2020. - : SPIE. - 1996-756X .- 0277-786X. - 9781510646186 ; 11886
  • Conference paper (peer-reviewed)abstract
    • We report on the use of multilayer Laue lenses to focus the intense X-ray Free Electron Laser (XFEL) beam at the European XFEL to a spot size of a few tens of nanometers. We present the procedure to align and characterize these lenses and discuss challenges working with the pulse trains from this unique X-ray source.
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