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- Ohlson, Sten, et al.
(författare)
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A Novel Approach to Monoclonal Antibody Separation Using High Performance Liquid Affinity Chromatography (HPLAC) with SelectiSpher-10 Protein G
- 1988
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Ingår i: JIM - Journal of Immunological Methods. - : Elsevier BV. - 0022-1759 .- 1872-7905. ; 114, s. 175-180
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Tidskriftsartikel (refereegranskat)abstract
- Protein G, a bacterial cell wall protein extracted from strains of Streptococci, has been employed as a ligand in high performance liquid affinity chromatography (HPLAC) for separation of monoclonal antibodies. Examples are given of rapid high-resolution separations of rat and mouse monoclonal antibodies belonging to various subclasses. In comparison with protein A chromatography, we were able to show superior binding characteristics for SelectiSpher-10 protein G columns under conditions of 'low' ionic strengh (about 0.1 M) and neutral pH (pH ≈ 7). The monoclonal antibodies were isolated in high purity (> 90%) and with good recovery of specific activity (80–100%).We believe that the HPLAC technology based on SelectiSpher-10 protein G is of potential value in the analysis and purification of monoclonal antibodies from various species and subclasses.
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