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Träfflista för sökning "WFRF:(Ojala T) srt2:(2010-2014)"

Sökning: WFRF:(Ojala T) > (2010-2014)

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  • Ojala, JRM, et al. (författare)
  • A novel scavenger receptor 5-based antibiotic-independent selection method for generation of stable recombinant protein-producing mammalian cell lines especially suitable for proteins affecting cell adhesion
  • 2012
  • Ingår i: BioTechniques. - : Future Science Ltd. - 1940-9818 .- 0736-6205. ; 53:4, s. 221-
  • Tidskriftsartikel (refereegranskat)abstract
    • The establishment of stable recombinant protein-producing mammalian cell lines is an expensive, time-consuming, tedious procedure. In some cases, expressed recombinant proteins have adverse effects on host cell function, including cell adhesion. Based on the adhesive properties of SCARA5, a scavenger receptor (SR) of the class A SR family, we developed a method for selection of stable recombinant protein-producing cell clones that relies on an internal ribosome entry site (IRES) vector where the protein of interest is expressed in the same messenger RNA as SCARA5, resulting in improved adhesion and increased cell viability of recombinant protein-producing cells in serum-free media. This method does not depend on antibiotics, complicated selective cell culture media or equipment, and thus offers the advantages of being inexpensive, environmentally friendly, and simple.
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  • Szczesniak, T., et al. (författare)
  • A Time Resolution Study of a Continuous Crystal Detector for TOF PET
  • 2010
  • Ingår i: IEEE Transactions on Nuclear Science. - 0018-9499 .- 1558-1578. ; 57:1, s. 40-47
  • Tidskriftsartikel (refereegranskat)abstract
    • The majority of current Positron Emission Tomography (PET) systems are based on block-detectors consisting of many scintillating pixels read by a smaller number of photomultipliers (PMTs). An improvement of the time resolution, using a common light readout from a cluster of PMTs, was proposed by Kuhn et al., and tested by us earlier with LSO crystals. This, triggered an idea to design a new PET detector optimized for Time of Flight (TOF) systems, based on continuous crystals. In the present work, we report on optimization of timing with a 20 x 20 x 20 mm(3) LYSO crystal coupled to a 16-channel photomultiplier H8711-200MOD from Hamamatsu. First, measurements were performed of the transit time jitter, the number of photoelectrons and the time resolution using a small 10 x 10 x 5 mm(3) LSO crystal coupled to a H8711-200MOD PMT. Results were compared with data collected from fast timing photomultipliers like Photonis XP1020, XP3060, XP20D0 or Hamamatsu R9800. In the second part of the study, the time resolution measurements and the optimization of the system were made with a continuous LYSO crystal. The final results are discussed in terms of the measured photoelectron number and the requirements for the TOF-PET scanners.
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