Sökning: WFRF:(Oliver Steven E)
> (2002-2004) >
Azadirachtin disrup...
Azadirachtin disrupts formation of organised microtubule arrays during microgametogenesis of Plasmodium berghei
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Billker, Oliver (författare)
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Shaw, Michael K (författare)
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Jones, Ian W (författare)
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Ley, Steven V (författare)
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Mordue, A Jennifer (författare)
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Sinden, Robert E (författare)
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- Wiley, 2002
- 2002
- Engelska.
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Ingår i: Journal of Eukaryotic Microbiology. - : Wiley. - 1066-5234 .- 1550-7408. ; 49:6, s. 489-497
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Transmission of malaria parasites from vertebrate blood to the mosquito vector depends critically on the differentiation of the gametocytes into gametes. This occurs in response to environmental stimuli encountered by the parasite in the mosquito bloodmeal. Male gametogenesis involves three rounds of DNA replication and endomitosis, and the assembly de novo of 8 motile axonemes. Azadirachtin, a plant limnoid and insecticide with an unkown mode of action, specifically inhibits the release of motile gametes from activated microgametocytes but does not inhibit growth and replication of a sexual blood stages. We have combined confocal laser scanning microscopy and transmission electron microscopy to examine the effect of azadirachtin on the complex reorganisation of the microtubule cytoskeleton during gametogenesis in Plasmodium berghei. Neither the replication of the genome nor the ability of tubulin monomers to assemble into microtubules upon gametocyte activation were prevented by azadirachtin. However, the drug interfered with the formation of mitotic spindles and with the assembly of microtubules into typical axonemes. Our observations suggest that azadarachtin specifically disrupts the patterning of microtubules into more complex structures, such as mitotic spindles and axonemes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Animals
- Insecticides/*pharmacology
- Limonins/*pharmacology
- Microscopy
- Confocal
- Microscopy
- Electron
- Microtubules/*drug effects/metabolism/ultrastructure
- Plasmodium berghei/drug effects/*growth & development
- Spindle Apparatus/drug effects/metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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