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Träfflista för sökning "WFRF:(Olsson Per) srt2:(1970-1979)"

Sökning: WFRF:(Olsson Per) > (1970-1979)

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1.
  • Belfrage, Per, et al. (författare)
  • Dispersion of viable pig liver cells with collagenase
  • 1975
  • Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
  • Tidskriftsartikel (refereegranskat)abstract
    • Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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  • Malmström, Per, et al. (författare)
  • Cholecystomy for acute choelcystitis
  • 1973
  • Ingår i: The American Journal of Surgery. - 1879-1883. ; 126:3, s. 397-402
  • Tidskriftsartikel (refereegranskat)abstract
    • During the decade 1957 through 1966, sixty-three cholecystostomies in sixty-two patients were performed in the Surgical Department, University of Lund. Fifty-five of these sixty-two patients were followed up. The mean age was seventy-one years for both sexes. The primary mortality was 20 per cent: a third died of circulatory and respiratory insufficiency; three quarters died of peritonitis, abscess, and renal failure; half of the surviving patients had delayed wound healing. The mean hospital stay for the survivors was nineteen days. During the follow-up period, sixteen later required cholecystectomy, nine electively and seven for a recurrence of acute cholecystitis. Nine had another attack of cholecystitis after cholecystostomy. Another four had continuous symptoms; five more died of the gallbladder disease.It is concluded that cholecystostomy should be restricted to very ill patients and should be planned and carried out with the patient under local anesthesia. In all other patients, early primary cholecystectomy should be performed with cholangiography performed during the operation.
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