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PP15 89Zr-Sideropho...
PP15 89Zr-Siderophore-Affibody conjugates for imaging EGFR expression
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- Summer, D (författare)
- Department of Nuclear Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria
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- Garousi, Javad (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Oroujeni, Maryam, PhD, 1982- (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Mitran, Bogdan (författare)
- Uppsala universitet,Theranostics
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- Andersson, K. G. (författare)
- Division of Protein Technology, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden
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- Vorobyeva, Anzhelika (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Löfblom, J.n (författare)
- Division of Protein Technology, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden
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- Orlova, Anna, 1960- (författare)
- Uppsala universitet,Theranostics
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- Tolmachev, Vladimir (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Decristoforo, C (författare)
- Department of Nuclear Medicine, Medical University Innsbruck, Anichstrasse 35, A-6020 Innsbruck, Austria
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(creator_code:org_t)
- 2018-01-23
- 2018
- Engelska.
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Ingår i: EJNMMI Research. - : Springer Science and Business Media LLC. - 2191-219X. ; 8:S1
- Relaterad länk:
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https://ejnmmires.sp...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Aim: Zirconium-89 has gained great interest for PET, when imaging at late time points is required. Desferrioxamine B (DFO), is mostly used for this radionuclide as bifunctional chelator (BFC) and we recently reported on fusarinine C (FSC) with similar zirconium-89 complexing properties but potentially higher stability related to its cyclic structure. This study reports on the comparison of FSC and DFO as BFCs for 89Zr labelling of the affibody ZEGFR:2377 targeting Epidermal Growth Factor Receptors (EGFR).Methods: FSC-ZEGFR:2377 and DFO-ZEGFR:2377 were evaluated regarding labeling, in vitro stability, specificity, cell uptake, receptor affinity, biodistribution and microPET-CT imaging.Results: Both conjugates showed increased labelling yields at elevated temperature (85°C). Both conjugates revealed remarkable specificity, affinity and slow cell-line dependent internalisation. Labeling at 85°C showed comparable results in A431 tumor xenografted mice with minor differences regarding blood clearance, tumor and liver uptake but clear improvement as compared to 89Zr-DFO-ZEGFR:2377, labeled at room temperature, which was confirmed by MicroPET-CT imaging.Conclusion: We were able to show that FSC is a suitable alternative to DFO for labeling of biomolecules with zirconium-89. Furthermore our findings indicate that 89Zr- labeling of DFO conjugates at higher temperature reduces off-chelate binding leading to significantly improved tumor-to-organ ratios and therefore enhancing image contrast.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
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Summer, D
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Garousi, Javad
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Oroujeni, Maryam ...
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Mitran, Bogdan
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Andersson, K. G.
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Vorobyeva, Anzhe ...
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visa fler...
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Löfblom, J.n
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Orlova, Anna, 19 ...
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Tolmachev, Vladi ...
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Decristoforo, C
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visa färre...
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