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- Fagerberg, Linn, et al.
(författare)
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Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
- 2014
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 13:2, s. 397-406
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Tidskriftsartikel (refereegranskat)abstract
- Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody- based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to 80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.
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| 2. |
- Fagerberg, Linn, et al.
(författare)
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Contribution of antibody-based protein profiling to the human chromosome-centric proteome project (C-HPP)
- 2013
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 12:6, s. 2439-2448
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Tidskriftsartikel (refereegranskat)abstract
- A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approximately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).
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| 8. |
- Ottosson, Elisabet, et al.
(författare)
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Species associations during the succession of wood-inhabiting fungal communities
- 2014
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Ingår i: Fungal ecology. - : Elsevier BV. - 1754-5048 .- 1878-0083. ; 11, s. 17-28
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Tidskriftsartikel (refereegranskat)abstract
- We studied fungal succession in decaying wood by compiling time-series data of fruit body observations. We tested the hypothesis that the presence of a primary species affects the probability of a succeeding species occurring later on the same log. Significant associations were detected for 15 species pairs; these were consistent with earlier findings on cooccurrence patterns in single time surveys. We used enrichment analysis to test if species with particular life-history attributes were more often associated with the occurrence of a succeeding species, or vice versa. White rot fungi and fungi abundant as mycelia were more often associated with the occurrence of succeeding species, compared to brown rot fungi and species with low mycelial abundance. Our results indicate that certain primary species cause priority effects and non-random co-occurrence patterns in the field. These successional patterns are likely to be connected both with substrate modification and species interactions.
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| 9. |
- Ottosson, Niklas, et al.
(författare)
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Cations Strongly Reduce Electron Hopping-Times in Aqueous Solutions
- 2011
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 133:34, s. 13489-13495
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Tidskriftsartikel (refereegranskat)abstract
- We study how the ultrafast electron transfer between H2O molecules in liquid water upon absorption of soft X-ray radiation depends on the local molecular binding environment. Our probe is the resonant Auger-decay of the water O1s core-hole (~3.6 fs), by which we show that efficiency for electron delocalization can be significantly reduced when a first-shell water molecule is replaced by an atomic ion. Decays resulting from excitations at the O1s post-edge feature (~540 eV) of 6m LiBr and 3m MgBr2 aqueous solutions reveal electron hopping-times of approximately 1.5 and 1.9 fs, respectively – the latter represents a four-fold increase compared to the corresponding value in neat water. The slower electron delocalization in electrolytes, which shows a strong dependence on the charge of the cations, can be explained by ion-induced reduction of water-water orbital mixing. Density functional theory (DFT) electronic structure calculations of solvation geometries obtained from molecular dynamics simulations reveal that this phenomenon largely arises from electrostatic perturbations of ions on the solvating water molecules. Our results demonstrate that it is possible to deliberately manipulate charge-transfer rates in aqueous media.
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| 10. |
- Rohdin, Patrik, 1977-, et al.
(författare)
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Energy efficient process ventilation in paint shops in the car industry : Experiences and an evaluation of a full scale implementation at Saab Automobile in Sweden.
- 2012
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Ingår i: Ventilation 2012. ; , s. 1-6
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Konferensbidrag (refereegranskat)abstract
- Support processes in industrial energy systems, such as heating, ventilation and cooling systems, are important processes in industrial premises as they are related to energy cost, product quality as well as the indoor environment.In the vehicle production process the paint shop is the most energy intensive part, and about 75% of the energy is used in the ovens and spray booths. The spray booth line, which includes paint application and the oven, uses large quantities of air in order to keep the air quality in an optimal range to achieve the desired paint quality. The approach used in paint shops has up to now been to keep as much of steady state conditions as possible to avoid paint defects due to disturbances in the balance. This means that these high air flows are used also at low and non production hours. There is thus a large potential to increase energy efficiency by controlling the air flow and heating without losing the critical balances. This paper will present an initial post-implementation evaluation of the energy efficiency potential and experiences after running this type of system. CFD has been used to investigate the control strategy.
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