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- Hilson, P., et al.
(författare)
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Versatile gene-specific sequence tags for Arabidopsis functional genomics : Trancript profiling and reverse genetics applications
- 2004
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Ingår i: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 14:10B, s. 2176-2189
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Tidskriftsartikel (refereegranskat)abstract
- Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
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2. |
- Gaite, José A., et al.
(författare)
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Nonlinear spherical gravitational downfall of gas onto a solid ball : Analytic and numerical results
- 2003
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Ingår i: Physica D. - : Elsevier. - 0167-2789 .- 1872-8022. ; 183:1-2, s. 102-116
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Tidskriftsartikel (refereegranskat)abstract
- The process of downfall of initially homogeneous gas onto a solid ball due to the ball's gravity (relevant in astrophysical situations) is studied with a combination of analytic and numerical methods. The initial explicit solution soon becomes discontinuous and gives rise to a shock wave. Afterwards, there is a crossover between two intermediate asymptotic similarity regimes, where the shock wave propagates outwards according to two self-similar laws, initially accelerating and eventually decelerating and vanishing, leading to a static state. The numerical study allows one to investigate in detail this dynamical problem and its time evolution, verifying and complementing the analytic results on the initial solution, intermediate self-similar laws and static long-term solution.
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