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- Frantz, Laurent A. F., et al.
(author)
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Ancient pigs reveal a near-complete genomic turnover following their introduction to Europe
- 2019
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 116:35, s. 17231-17238
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Journal article (peer-reviewed)abstract
- Archaeological evidence indicates that pig domestication had begun by similar to 10,500 y before the present ( BP) in the Near East, and mitochondrial DNA ( mtDNA) suggests that pigs arrived in Europe alongside farmers similar to 8,500 y BP. A few thousand years after the introduction of Near Eastern pigs into Europe, however, their characteristic mtDNA signature disappeared and was replaced by haplotypes associated with European wild boars. This turnover could be accounted for by substantial gene flow from local European wild boars, although it is also possible that European wild boars were domesticated independently without any genetic contribution from the Near East. To test these hypotheses, we obtained mtDNA sequences from 2,099 modern and ancient pig samples and 63 nuclear ancient genomes from Near Eastern and European pigs. Our analyses revealed that European domestic pigs dating from 7,100 to 6,000 y BP possessed both Near Eastern and European nuclear ancestry, while later pigs possessed no more than 4% Near Eastern ancestry, indicating that gene flow from European wild boars resulted in a near-complete disappearance of Near East ancestry. In addition, we demonstrate that a variant at a locus encoding black coat color likely originated in the Near East and persisted in European pigs. Altogether, our results indicate that while pigs were not independently domesticated in Europe, the vast majority of human-mediated selection over the past 5,000 y focused on the genomic fraction derived from the European wild boars, and not on the fraction that was selected by early Neolithic farmers over the first 2,500 y of the domestication process.
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| 3. |
- Gela, Anele, et al.
(author)
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Eotaxin-3 (CCL26) exerts innate host defense activities that are modulated by mast cell proteases
- 2015
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In: Allergy. - : Wiley. - 1398-9995 .- 0105-4538. ; 70:2, s. 161-170
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Journal article (peer-reviewed)abstract
- BackgroundDuring bacterial infections of the airways, a Th1-profiled inflammation promotes the production of several host defense proteins and peptides with antibacterial activities including -defensins, ELR-negative CXC chemokines, and the cathelicidin LL-37. These are downregulated by Th2 cytokines of the allergic response. Instead, the eosinophil-recruiting chemokines eotaxin-1/CCL11, eotaxin-2/CCL24, and eotaxin-3/CCL26 are expressed. This study set out to investigate whether these chemokines could serve as innate host defense molecules during allergic inflammation. MethodsAntibacterial activities of the eotaxins were investigated using viable count assays, electron microscopy, and methods assessing bacterial permeabilization. Fragments generated by mast cell proteases were characterized, and their potential antibacterial, receptor-activating, and lipopolysaccharide-neutralizing activities were investigated. ResultsCCL11, CCL24, and CCL26 all showed potent bactericidal activity, mediated through membrane disruption, against the airway pathogens Streptococcus pneumoniae, Staphylococcus aureus, Nontypeable Haemophilus influenzae, and Pseudomonas aeruginosa. CCL26 retained bactericidal activity in the presence of salt at physiologic concentrations, and the region holding the highest bactericidal activity was the cationic and amphipathic COOH-terminus. Proteolysis of CCL26 by chymase and tryptase, respectively, released distinct fragments of the COOH- and NH2-terminal regions. The COOH-terminal fragment retained antibacterial activity while the NH2-terminal had potent LPS-neutralizing properties in the order of CCL26 full-length protein. An identical fragment to NH2-terminal fragment generated by tryptase was obtained after incubation with supernatants from activated mast cells. None of the fragments activated the CCR3-receptor. ConclusionsTaken together, the findings show that the eotaxins can contribute to host defense against common airway pathogens and that their activities are modulated by mast cell proteases.
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| 4. |
- Pease, Victoria, et al.
(author)
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U–Pb zircon geochronology of Cretaceous arc magmatism in eastern Chukotka, NE Russia, with implications for Pacific plate subduction and the opening of the Amerasia Basin
- 2018
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In: Geological Society Special Publication. - 0305-8719 .- 2041-4927. ; 460:1
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Journal article (peer-reviewed)abstract
- The tectonomagmatic evolution of eastern Chukotka, NE Russia, is important for refining the onset of Pacific plate subduction, understanding the development of the Amerasia Basin, and constraining Arctic tectonic reconstructions. Field mapping and strategic sample collection provide relative age constraints on subduction-related continental arc magmatism in eastern Chukotka. Ion microprobe U–Pb zircon ages provide absolute constraints and identify five magmatic episodes (c. 134, 122, 105, 94 and 85 Ma) separated by three periods of uplift and erosion (c. 122–105, 94–85 and post-85 Ma). Volcanic rocks in the region are less contaminated than their plutonic equivalents which record greater crustal assimilation. These data, combined with xenocrystic zircons, reflect the self-assimilation of a continental arc during its evolution. Proto-Pacific subduction initiated by c. 121 Ma and arc development occurred over c. 35–50 myr. Crustal growth was simultaneous with regional exhumation and crustal thinning across the Bering Strait region. Ocean–continent subduction in eastern Chukotka ended at c. 85 Ma. The timing of events in the region is roughly synchronous with the inferred opening of the Amerasia Basin. Simultaneous arc magmatism, extension and development of the Amerasia Basin within a back-arc basin setting best explain these coeval tectonic events.
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