| 1. |
- Bhalerao, RP, et al.
(författare)
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Cloning of the phycobilisome rod linker genes from the cyanobacterium synechococcus sp pcc-6301 och their inactivation in synechococcus sp pcc-7942
- 1993
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Ingår i: Molecular General Genetics. - 0026-8925 .- 1432-1874. ; 237:1-2, s. 89-96
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Tidskriftsartikel (refereegranskat)abstract
- The phycobilisome rod linker genes in the two closely related cyanobacteria Synechococcus sp. PCC 6301 and Synechococcus sp. PCC 7942 were studied. Southern blot analysis showed that the genetic organization of the phycobilisome rod operon is very similar in the two strains. The phycocyanin gene pair is duplicated and separated by a region of about 2.5 kb. The intervening region between the duplicated phycocyanin gene pair was cloned from Synechococcus sp. PCC 6301 and sequenced. Analysis of this DNA sequence revealed the presence of three open reading frames corresponding to 273, 289 and 81 amino acids, respectively. Insertion of a kanamycin resistance cassette into these open reading frames indicated that they corresponded to the genes encoding the 30, 33 and 9 kDa rod linkers, respectively, as judged by the loss of specific linkers from the phycobilisomes of the insertional mutants. Amino acid compositions of the 30 and 33 kDa linkers derived from the DNA sequence were found to deviate from those of purified 33 and 30 kDa linkers in the amounts of glutamic acid/glutamine residues. On the basis of similarity of the amino acid sequence of the rod linkers between Synechococcus sp. PCC 6301 and Calothrix sp. PCC 7601 we name the genes encoding the 30, 33 and 9 kDa linkers cpcH, cpcI and cpcD, respectively. The three linker genes were found to be co-transcribed on an mRNA of 3700 nucleotides. However, we also detected a smaller species of mRNA, of 3400 nucleotides, which would encode only the cpcH and cpcI genes. The 30 kDa linker was still found in phycobilisome rods lacking the 33 kDa linker and the 9 kDa linker was detected in mutants lacking the 33 or the 30 kDa linkers. Free phycocyanin was found in the mutants lacking the 33 or the 30 kDa linkers, whereas no free phycocyanin could be found in the mutant lacking the 9 kDa linker.
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| 2. |
- Persson, Johan, et al.
(författare)
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Prediction of surface water turnover time in coastal waters using digital bathymetric information
- 1994
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Ingår i: Environmetrics. - : Wiley. - 1180-4009 .- 1099-095X. ; 5:4, s. 433-449
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a new type of geographical information system (GIS) for scientific planning of coastal waters. One hypothesis in the work is that the morphometry of the coast plays a significant role for how the water system functions as a receiving water system, for example, as a receiver of industrial and urban pollution and in response to various forms of aquaculture. A digital technique for transferring information from standard charts into morphometric parameters expressing various characteristics of the coast has been developed. Empirical data on surface water turnover times, which are costly and demanding to determine with traditional hydrodynamic methods, has been obtained from the literature for 20 defined Swedish coastal areas. Two models for simple predictions of the median surface water turnover time, based on morphometric parameters, have been developed to exemplify the use of “morphometrical models” in expressing a coast ecological key parameter. In these models, more than 90 per cent of the variation in empirical values of surface water turnover times can be statistically explained by the topographic openness. The topographic openness describes the exposure of the coastal area towards the open sea or adjacent coastal area. The models are valid for the temperature stratified period (May–October) in areas non‐affected by tides, strong coastal currents and river inflow. The areas should also be in the size range 0.15–150 km2.
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