| 1. |
- Henriksson, Elisabet Welin, 1960-, et al.
(författare)
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Autoepitope-mapping of the U1-70K protein with human-Drosophila chimeric proteins
- 1997
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Ingår i: Journal of Autoimmunity. - : Academic Press. - 0896-8411 .- 1095-9157. ; 10:6, s. 559-568
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Tidskriftsartikel (refereegranskat)abstract
- The 70K protein is the major autoantigen for anti-RNP autoantibodies directed against the U1 small nuclear ribonucleoprotein complex particle. The U1-70K protein has been epitope-mapped by various groups, and a major antigenic region of about 70 amino acids has been found which overlaps with the RNA binding motif. Attempts to map the major antigenic region further with smaller cloned fragments or with peptides have been hampered by total loss of, or strongly reduced, antigenicity. Thus the major antigenic region is composed of conformational epitopes and a detailed analysis of particular epitopes has not been possible. In the present work, we examine the antigenicity of chimeric proteins assembled from the highly conserved Drosophila melanogaster 70K proteins grafted with human 70K segments. With this approach, the effects on antigenicity of exchanging particular segments can be assayed with the overall structure of the major antigenic domain kept relatively constant. Our results, supported by depletion experiments, show that residues 99-128 from the human protein are essential for recognition by both human and canine anti-RNP autoantibodies. These residues have to be presented in a manner that allows correct conformational interaction between the different protein domains.
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| 2. |
- Henriksson, Elisabet Welin, 1960-, et al.
(författare)
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Human anti-RNP sera contain both human-specific and cross-reactive anti-70K autoantibodies
- 1996
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Ingår i: Journal of Autoimmunity. - : Academic Press. - 0896-8411 .- 1095-9157. ; 9:4, s. 551-559
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Tidskriftsartikel (refereegranskat)abstract
- The U1 snRNP (small nuclear ribonucleoprotein complex) associated 70K protein is the main autoantigen for the anti-RNP autoantibodies which are directed against the U1 snRNP particle. The major antigenic region of the 70K protein has by various laboratories been mapped to an RNA binding domain required for the 70K-U1 snRNA interaction. We have used recombinant proteins comprising this region from the human and the Drosophila melanogaster 70K proteins to examine the species specificity of the human anti-70K autoantibodies found in 42 patient sera. Most, but not all, anti-70K positive sera in this cross-sectional sample contained both human 70K specific anti-bodies and Drosophila 70K reactive antibodies. Results of a longitudinal follow-up of 14 patients indicated that the cross-reactive anti-70K antibodies developed secondarily to the establishment of a species-specific anti-70K reaction. In a fraction of the patient sera this broadening of the response never occurred. Taken together, the data in this study support the hypothesis that the endogenous human 70K protein is the immunogen driving the production of anti-70K autoantibodies.
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| 3. |
- Håkansson, Lars, et al.
(författare)
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Active Control Machine Tool Chatter Embedded Piezo Ceramic Actuators in Tool Holder Shank
- 1999
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Konferensbidrag (refereegranskat)abstract
- In the turning operation chatter or vibration is a frequent problem, which affects the result of the machining, and, in particular, the surface finish. Tool life is also influenced by vibration. Severe acoustic noise in the working environment frequently occurs as a result of dynamic motion between the cutting tool and the workpiece. These problems can be reduced by active control of machine-tool vibration. Adaptive feedback control based on the filtered-x LMS-algorithm, enables a reduction of the vibration by up to 40 dB at 1.5 kHz and by approximately 40 dB at 3 kHz. The active control performed a broadband attenuation of the sound pressure level by up to 35 dB . A significant improvement of the workpiece surface was also observed. In the active control of tool vibration a tool holder construction based on integrated high magnetostrictive actuators was used. However, both the physical features and properties of an active tool holder construction based on high magnetostrictive actuators and the fact that this type of actuators generally have a non-linear behaviour highly reduce its applicability to the general lathe and turning operation. Therefor, a new generation embedded active tool holder shanks based on piezo ceramic actuators have been developed. Based on spectrum estimates, both coherence spectrum and frequency response function estimates has been calculated for both the old tool holder construction and the new generation active tool holder shank. From the results it follows that the phase delay is smaller and the linearity of the new generation active tool holder shank are superior compared to the old technology. It is also obvious that physical features and properties of new generation embedded active tool holder shanks based on piezo ceramic actuators fits the general lathe application.
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| 4. |
- Håkansson, Lars, et al.
(författare)
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Embedded Piezoceramic Actutaors in a Tool Holder Shank for the Active Control of Machine-tool Vibration
- 1999
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Konferensbidrag (refereegranskat)abstract
- In the turning operation chatter or vibration is a frequent problem, which affects the result of the machining, and, in particular, the surface finish. Tool life is also influenced by vibration. Severe acoustic noise in the working environment frequently occurs as a result of dynamic motion between the cutting tool and the workpiece. These problems can be reduced by active control of machine-tool vibration based on high magnetostrictive actuators. However, both the physical features and properties of a active tool holder construction based on high magnetostrictive actuators and the fact that this type of actuators generally have a non-linear behaviour highly reduce its applicability to the general lathe and turning operation. Therefor, a new generation embedded active tool holder shanks based on piezo ceramic actuators have been developed. Based on spectrum estimates, both coherence spectrum and frequency response function estimates has been calculated for both the old tool holder construction and the new generation active tool holder shank. From the results it follows that the phase delay is smaller and the linearity of the new generation active tool holder shank are superior compared to the old technology. It is also obvious that physical features and properties of new generation embedded active tool holder shanks based on piezo ceramic actuators fits the general lathe application.
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| 5. |
- Pettersson, Börge, et al.
(författare)
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Etnobiologi i Sverige
- 1998
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Ingår i: Fauna och Flora. ; 93, s. 145-148
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 6. |
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| 7. |
- Pettersson, Linus, et al.
(författare)
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Detection in a Robotised Short Circuting GMA Welding using Neural Networks
- 1999
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Konferensbidrag (refereegranskat)abstract
- Today it is both time and cost consuming to check the quality of a weld when it is done off-line by an experienced weld operator. Therefor the need for an automatic detection is urgent in order to reduce production costs. There are systems for monitoring the quality of a weld commercially available but there is still some research that has to be done in this area in order to increase the reliability. The method presented in this paper is neural network based and considers short circuiting GMA welding, but there is no obstacle for this solution to work on other types of robotised welding. By presenting the weld voltage to a neural network, the network is able to detect defects in the weld joint. Testresults have shown that the detection rate is 100 percent and false alarms are nonexisting.
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| 8. |
- Svanberg, Ingvar & Pettersson, Börge & Tunón, Håkan
(författare)
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Människan och bävern
- 1998
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Ingår i: Biodiverse från Centrum för biologisk mångfald. ; 3:2, s. 12-13
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- En kortfattad etnobiologisk monografi över bäverns roll i Sverige förr och nu. Här diskuteras folkliga föreställningar, användningen av bäverskinn, jakt och fångst av bäver förr och nu, bäverns olika benämningar, återintroduceringen av bäver samt bävergal
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| 9. |
- Svanberg, Ingvar & Pettersson, Börge & Tunón, Håkan
(författare)
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Nötter: etnobiologisk läsebok
- 1998
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- I det förindustriella Sverige tjänade nötter som omväxling och helgdagsgotter. Det var ett uppskattat nöje för ungdomar att få ge sig ut i hagarna och skörda hasselnötter på hösten. Nötlekar under julen var populärt. I dag utgör nötter en viktig del av vå
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| 10. |
- Welin Henriksson, Elisabet, 1960-, et al.
(författare)
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Autoantibody profiles in canine ANA-positive sera investigated by immunoblot and ELISA
- 1998
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Ingår i: Veterinary Immunology and Immunopathology. - : Elsevier. - 0165-2427 .- 1873-2534. ; 61:2-4, s. 157-170
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Tidskriftsartikel (refereegranskat)abstract
- Canine systemic lupus erythematosus (SLE) has a similar disease expression as human SLE, but the serological characterisation of the canine disease is as yet incomplete. In the present study, we examined the specificity of antinuclear antibodies (ANA) in indirect immunofluorescence (IIF) positive canine sera. Sixty-four canine IIF ANA positive sera were characterised using HeLa cell nuclear extract immunoblots and recombinant U1-70K ELISA. We compared these results with a previously shown concordance between indirect immunofluorescence and immunodiffusion in canine SLE serological diagnosis. One canine serum reacting with Sm proteins was observed, and five canine sera presented anti-RNP autoantibodies against the antigens 70K, A, C, and/or B/B'. The autoantigen most frequently recognised was a 43 kDa nuclear protein, previously described as hnRNP G. This prominent canine autoantigen was missing in the commercially available extract designed for immunodiffusion testing of human sera. Other prominent canine autoantigens were found not to be identical with the principal human ones, thus making present human test systems deficient for the use in canine systemic connective disease diagnosis. The development of antigenic extract designed for canine autoimmune autoantigens is necessary in order to make immunodiffusion a useful method in canine diagnosis. The anti-RNP positive canine sera were examined in more detail and we found that the human major antigenic region of the most prominent RNP antigen, the U1-70K protein, also is targeted by canine autoantibodies. Thus, the response against the RNP antigen seems to be conserved between man and dog.
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