| 1. |
- Tiukova, Ievgeniia, et al.
(författare)
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Transcriptome of the Alternative Ethanol Production Strain Dekkera bruxellensis CBS 11270 in Sugar Limited, Low Oxygen Cultivation
- 2013
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:3, s. e58455-
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Tidskriftsartikel (refereegranskat)abstract
- Dekkera bruxellensis can outcompete Saccharomyces cerevisiae in environments with low sugar concentrations. It is usually regarded as a spoilage yeast but has lately been identified as an alternative ethanol production organism. In this study, global gene expression in the industrial isolate D. bruxellensis CBS 11270 under oxygen and glucose limitation was investigated by whole transcriptome sequencing using the AB SOLiD technology. Among other observations, we noted expression of respiratory complex I NADH-ubiquinone reductase although D. bruxellensis is a Crabtree positive yeast. The observed higher expression of NADH-generating enzymes compared to NAD(+)-generating enzymes might be the reason for the previously observed NADH imbalance and resulting Custer effect in D. bruxellensis. Low expression of genes involved in glycerol production is probably the molecular basis for high efficiency of D. bruxellensis metabolism under nutrient limitation. No D. bruxellensis homologs to the genes involved in the final reactions of glycerol biosynthesis were detected. A high number of expressed sugar transporter genes is consistent with the hypothesis that the competitiveness of D. bruxellensis is due to a higher affinity for the limiting substrate.
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| 2. |
- Aftab, Obaid, 1984-, et al.
(författare)
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NMR spectroscopy based metabolic profiling of drug induced changes in vitro can discriminate between pharmacological classes
- 2014
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Ingår i: Journal of chemical information and modeling. - : American Chemical Society (ACS). - 1549-9596 .- 1549-960X. ; 54:11, s. 3251-3258
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Tidskriftsartikel (refereegranskat)abstract
- Drug induced changes in mammalian cell line models have already been extensively profiled at the systemic mRNA level and subsequently used to suggest mechanisms of action for new substances as well as to support drug repurposing, i.e. identifying new potential indications for drugs already licensed for other pharmacotherapy settings. The seminal work in this field, which includes a large database and computational algorithms for pattern matching, is known as the “Connectivity Map” (CMap). The potential of similar exercises at the metabolite level is, however, still largely unexplored. Only recently the first high throughput metabolomic assay pilot study was published, involving screening of metabolic response to a set of 56 kinase inhibitors in a 96-well format. Here we report results from a separately developed metabolic profiling assay, which leverages 1H NMR spectroscopy to the quantification of metabolic changes in the HCT116 colorectal cancer cell line, in response to each of 26 compounds. These agents are distributed across 12 different pharmacological classes covering a broad spectrum of bioactivity. Differential metabolic profiles, inferred from multivariate spectral analysis of 18 spectral bins, allowed clustering of most tested drugs according to their respective pharmacological class. A more advanced supervised analysis, involving one multivariate scattering matrix per pharmacological class and using only 3 spectral bins (three metabolites), showed even more distinct pharmacology-related cluster formations. In conclusion, this kind of relatively fast and inexpensive profiling seems to provide a promising alternative to that afforded by mRNA expression analysis, which is relatively slow and costly. As also indicated by the present pilot study, the resulting metabolic profiles do not seem to provide as information rich signatures as those obtained using systemic mRNA profiling, but the methodology holds strong promise for significant refinement.
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| 3. |
- Ahsan, Muhammad, et al.
(författare)
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Genetic analysis and replication of QTL effects on chicken body weight using a multi-locus introgression line
- 2013
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Ingår i: Journal of Data Mining in Genomics and Proteomics. - 2153-0602. ; 4, s. 83-83
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Quantitative Trait Loci (QTL) mapping is a useful initial step to dissect the geneticarchitecture of complex biological traits. Intercrosses between divergently selected lines ofanimals is a powerful approach to map loci that affect such traits individually or throughinteractions. The drawback with the approach is a low mapping resolution and consequentlyfurther fine mapping is needed for detailed studies of the mapped loci. We have earliermapped a network of four interacting loci that through reciprocal epistatic capacitationexplains nearly half of the difference in body weight between the Virginia High WeightSelected (HWS) and Low Weight Selected (LWS) chicken lines that after 50 generationsdisplay a 12-fold difference in weight at 56 days of age. The network has been replicated andfine-mapped in an eight-generation Advance Intercross Line (AIL) and its large effect onweight confirmed. A three-locus introgression line has been bred, where the LWS haplotypesat the three loci in the network with the strongest effects on weight have been simultaneouslyintrogressed into the HWS background. Here we describe the first results from a haplotypebasedassociation analysis in this multi-locus introgression line that again replicate the effectsof the introgressed loci on body-weight in the Virginia chicken lines, but also indicate anallelic heterogeneity at the loci within the founder-lines. Further analyses are in progress toevaluate the epistatic effects of the loci in this population as well as the functionalcontribution of the allelic heterogeneity.
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| 4. |
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| 5. |
- Andersson, Patrik U, 1970, et al.
(författare)
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Formation of Highly Rovibrationally Excited Ammonia from Dissociative Recombination of NH4
- 2010
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Ingår i: Journal of Physical Chemistry Letters. - : American Chemical Society (ACS). - 1948-7185. ; 1:17, s. 2519-2523
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Tidskriftsartikel (refereegranskat)abstract
- The internal energy distribution of ammonia formed in the dissociative recombination (DR) of NH4+ with electrons has been studied by an imaging technique at the ion storage ring CRYRING. The DR process resulted in the formation of NH3 + H (0.90 ± 0.01), with minor contributions from channels producing NH2 + H2 (0.05 ± 0.01) and NH2 + 2H (0.04 ± 0.02). The formed NH3 molecules were highly internally excited, with a mean rovibrational energy of 3.3 ± 0.4 eV, which corresponds to 70% of the energy released in the neutralization process. The internal energy distribution was semiquantitatively reproduced by ab initio direct dynamics simulations, and the calculations suggested that the NH3 molecules are highly vibrationally excited while rotational excitation is limited. The high internal excitation and the translational energy of NH3 and H will influence their subsequent reactivity, an aspect that should be taken into account when developing detailed models of the interstellar medium and ammonia-containing plasmas.
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| 6. |
- Andrén, Eva, et al.
(författare)
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Öppna prioriteringar inom nya områden : logopedi, nutritionsbedömning, habilitering och arbetsterapi
- 2011
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Det finns fortfarande ett behov av att öka kunskapen om och stödja den praktiska tillämpningen av riksdagens riktlinjer för öppna prioriteringar inom svensk hälso- och sjukvård. Flera förslag på hur ett sådant stöd kan se ut har tagits fram de senaste åren. Spridning av goda exempel är ett sådant förslag, metodstöd ett annat (PrioriteringsCentrum 2007). En mer påtaglig form av metodstöd är den nationella modell som vuxit fram för att konkretisera innebörden i riktlinjerna (Carlsson m fl 2007). Den får idag anses som välbeprövad inom ett flertal områden och har bidragit till att samsynen och kommunicerbarheten kring prioriteringar har ökat i landet. Erfarenheter visar dock att det behövs pedagogisk vägledning i hur modellen kan tillämpas. För att möta upp efterfrågan på sådant metodstöd erbjuder Prioriteringscentrum handledning i grupp. Den första handledningsgruppen är nu avslutad och det är deltagarnas prioriteringsarbeten som presenteras i denna rapport i syfte att sprida konkreta exempel på försök att tillämpa prioriteringsriktlinjerna.I rapporten presenteras fyra prioriteringsarbeten med fokus på: Regionsamverkan inom arbetsterapi Logopedi Yrkesspecifika prioriteringar på väg till teamet Från projekt till integrerat redskapExemplet med prioriteringar i regionsamverkan utgörs av det prioriteringsarbete som genomförts i det s k femklövernätverket bestående av en samverkansgrupp för arbetsterapeuter i ledningsposition på sjukhusen i Uppland, Västmanland, Södermanland, Gävleborg och Dalarna. Arbetet var ett försök att skapa gemensamma prioriteringar i regionen för ett sjukdomsområde som kändes relevant. Valet kom att falla på arbetsterapi inom reumatologi. Arbetet har sedan huvudsakligen bedrivits i en projektgrupp, bestående av en representant från varje sjukhus där arbetet växlat mellan arbete på hemmaplan och avstämningsträffar i projektgruppen.Försöket har visat att det finns en samsyn inom regionen kring prioriteringar inom arbetsterapi och reumatologi. Säkerheten i prioriteringarna har ökat i och med att fem arbetsterapiorganisationer tillsammans bidragit med ett stort underlagsmaterial bl a genom att delge varandra sina kliniska erfarenheter. Förutsättningarna för en mer likartad vård i regionen har ökat. Arbetet har också gett upphov till frågor om i vilka situationer det är att föredra att prioriteringsarbete bedrivs lokalt, regionvis och/eller nationellt.
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| 7. |
- Björklund, Svante, et al.
(författare)
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A three-dimensional displaced phase center antenna condition for clutter cancellation
- 2014
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Ingår i: Proceedings of the IEEE Sensor Array and Multichannel Signal Processing Workshop. - : IEEE. - 9781479914814 ; , s. 305-308
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Konferensbidrag (refereegranskat)abstract
- In moving radar, e.g. airborne radar, the clutter from land and sea needs to be suppressed in order to detect the target. One approach to total cancellation of the clutter is Displaced Phase Center Antenna (DPCA). DPCA assumes that the antenna elements are positioned on a line parallel to the velocity vector of the radar platform so that the elements can take each others positions at different points of times. This paper theoretically investigates if it is possible with other antenna element positions, e.g. in three dimensions, for a total cancellation of the clutter. We arrive at a condition which conforms to the principle that the elements should take each others positions at different times but allows other antennas than the single line parallel to the velocity vector. Our condition could be used as constraints in an optimization problem where the target signal performance is optimized. The multipulse DPCA condition is one solution to our condition.We also give two examples of non-linear antennas fulfilling our condition.
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| 8. |
- Björklund, Svante, et al.
(författare)
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Auxiliary beam terrain-scattered interference suppression : reflection system and radar performance
- 2013
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Ingår i: IET Radar, Sonar & Navigation. - : The Institution of Engineering and Technology (IET). - 1751-8784 .- 1751-8792. ; 7:8, s. 836-847
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Tidskriftsartikel (refereegranskat)abstract
- Terrain-scattered interference (TSI), that is, jammer signals reflected on the earth's surface, is a significant problem to military airborne radar. In auxiliary beam TSI suppression, the TSI in the main radar beam is estimated by a single or several auxiliary beams and is subtracted from the main beam channel. The signal to subtract is the auxiliary beam signals fed through an estimate of the ‘reflection system’, which describes scattering on the surface. The authors first present results on the structure of this TSI suppression, on the estimation of the reflection system and on the quality of the estimate. Then the authors derive theoretical expressions for the signal-to-interference plus noise ratio (SINR) and the remaining TSI power for a single auxiliary beam. Since the SINR is directly connected to the radar performance, it can be seen what factors affect the performance and how. It was noted that when the estimated reflection system is missing one or more delays of the true system, the TSI filter cannot suppress the TSI signal completely. This phenomenon, which is called ‘TSI leakage’, has a very large impact on the performance. The SINR cannot be kept constant. Instead, an ‘SINR improvement’ can be defined.
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| 9. |
- Burström, Lage, et al.
(författare)
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A follow-up study of welders’ exposure to vibration in a heavy engineering production workshop
- 2010
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Ingår i: Journal of Low Frequency Noise Vibration and Active Control. - : Sage Publications. - 0263-0923 .- 1461-3484 .- 2048-4046. ; 29:1, s. 33-39
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Tidskriftsartikel (refereegranskat)abstract
- Manual work involving vibrating power tools is associated with symptoms that include vascular, neurological and musculoskeletal disorders. This study examines the vibration exposure of welders to determine the change between 1987 and 2008. Vibration measurements on handheld tools were used to evaluate the acceleration and the daily exposure time was determined by subjective rating. From these data, the 8-hour equivalent vibration exposure A(8), has been calculated. During the period, the A(8) decreased from 3.9 m/s2 to 1.9 m/s2. It was concluded that this decrease is the result of fewer vibrating tools and a decrease in daily exposure time. Although the daily vibration exposure has decreased over the study time, for some welders the daily vibration exposure A(8) is still above the action value set by the EU directive on vibration. This means more effort should be spent to decrease vibration exposure.
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| 10. |
- Carnlöf, Carina, et al.
(författare)
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Health-related quality of life in patients with atrial fibrillation undergoing pulmonary vein isolation, before and after treatment
- 2010
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Ingår i: European Journal of Cardiovascular Nursing. - : Oxford University Press. - 1474-5151 .- 1873-1953. ; 9:1, s. 45-49
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Tidskriftsartikel (refereegranskat)abstract
- Background: Atrial fibrillation (AF) is the most common arrhythmia and many AF patients experience a significantly impaired health-related quality of life (HRQOL). AF is also associated with a high risk of stroke and death. Many pharmacologic treatments for AF are ineffective and may have adverse effects. New methods, such as pulmonary vein isolation (PVI), have been developed to treat AF.Aims: The aim of this study was to investigate the HRQOL issues in severe symptomatic AF patients before and after pulmonary vein isolation.Methods: Forty patients treated with PVI were included of which 36 concluded the study with the self-reported HRQOL questionnaires before and once after PVI. A standardized control group was used.Results: Compared to the control group the HRQOL before PVI was significantly lower in all domains except for bodily pain. The preoperative scores were compared with the scores obtained at the follow-up. All subscales of the SF-36 significantly improved after the PVI except for bodily pain, which remained unaltered.Conclusion: HRQOL is improved in AF patients with severe symptoms after PVI intervention.
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