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| 2. |
- Hanssen, K. E., et al.
(författare)
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The effect of strength training volume on satellite cells, myogenic regulatory factors, and growth factors
- 2013
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Ingår i: Scandinavian Journal of Medicine and Science in Sports. - Hoboken, USA : Wiley-Blackwell. - 0905-7188 .- 1600-0838. ; 23:6, s. 728-739
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this work was to study the effect of training volume on activation of satellite cells. Healthy untrained men were randomly assigned into two groups. The 3L-1UB group (n = 10) performed three-set leg exercises and single-set upper body exercises, and the 1L-3UB group (n = 11) performed single-set leg exercises and three-set upper body exercises. Both groups performed three sessions (80-90 min) per week for 11 weeks. Biopsies were taken from m. vastus lateralis and m. trapezius. The number of satellite cells, satellite cells positive for myogenin and MyoD, and the number of myonuclei were counted. Homogenized muscle was analyzed for myogenin and MyoD, and extracted ribonucleic acid (RNA) was monitored for selected growth factor transcripts. Knee extensor strength increased more in the 3L-1UB group than in the 1L-3UB group (48 ± 4% vs 29 ± 4%), whereas the strength gain in shoulder press was similar in both training groups. The number of satellite cells in m. vastus lateralis increased more in the 3L-1UB group than in the 1L-3UB group. The number of myonuclei increased similarly in both groups. The messenger RNA expression of growth factors peaked after 2 weeks of training. In conclusion, increasing training volume enhanced satellite cell numbers in the leg muscle, but not in the upper body muscle.
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| 3. |
- Andersson, Helena M., et al.
(författare)
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Differences in the inflammatory plasma cytokine response following two elite female soccer games separated by a 72-h recovery
- 2010
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Ingår i: Scandinavian Journal of Medicine and Science in Sports. - Malden, USA : Wiley-Blackwell. - 0905-7188 .- 1600-0838. ; 20:5, s. 740-747
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Tidskriftsartikel (refereegranskat)abstract
- We investigated changes in a large battery of pro- and anti-inflammatory cytokines in elite female soccer players following two 90-min games separated by a 72-h active or passive recovery. Blood samples were taken from 10 players before, within 15-20 min, 21, 45 and 69 h after the first game and within 15-20 min after the second game. The leukocyte count was analyzed, together with several plasma pro- and anti-inflammatory cytokines, using a multiplex bead array system. After the first and second game, the total leukocytes and neutrophils increased significantly. Likewise, increases (P<0.05) in pro-inflammatory cytokines [interleukin (IL)-12, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (INF-gamma), IL-17], chemokines [monocyte chemotactic protein-1 (MCP-1), IL-8 and monokine induced by gamma interferon (MIG)], anti-inflammatory cytokines (IL-2R, IL-4, IL-5, IL-7, IL-10, IL-13, INF-alpha) and the mixed cytokine IL-6 were observed. Leukocyte and cytokine levels were normalized within 21 h. Active recovery (low-intensity exercises) did not affect the cytokine responses. A dampened cytokine response was observed after the second game as only IL-12, IL-6, MCP-1, IL-8 and MIG increased (P<0.05). In conclusion, a robust pro- and anti-inflammatory cytokine response occurs after the first but not the second soccer game. The implications of the dampened cytokine response in female players after the second game are unknown.
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| 4. |
- Andersson, Helena M., et al.
(författare)
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Plasma antioxidant responses and oxidative stress following a soccer game in elite female players
- 2010
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Ingår i: Scandinavian Journal of Medicine and Science in Sports. - Malden, USA : Wiley-Blackwell. - 0905-7188 .- 1600-0838. ; 20:4, s. 600-608
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Tidskriftsartikel (refereegranskat)abstract
- We aimed to investigate markers of oxidative stress and levels of endogenous and dietary antioxidants in 16 elite female soccer players in response to a 90-min game (average intensity 82+/-3% HRpeak). Blood samples were taken before, immediately and 21 h after the game. Plasma-oxidized glutathione, the ratio of reduced to oxidized glutathione (GSH:GSSG) and lipid peroxidation measured by d-ROMs were used as markers of oxidative stress. Plasma endogenous [uric acid, total glutathione (TGSH)] and dietary antioxidants (alpha-tocopherol, ascorbic acid, total carotenoids and polyphenols) were analyzed using liquid chromatography and the Folin-Ciocalteu method. Exercise induced an acute increase (P<0.05) in GSSG, uric acid, TGSH, alpha-tocopherol, and ascorbic acid. In parallel, the GSH:GSSG ratio and polyphenols decreased (P<0.05). GSSG, GSH:GSSG ratio, uric acid, TGSH, and ascorbic acid returned to baseline at 21 h, while polyphenols and alpha-tocopherol remained altered. Total carotenoids increased above baseline only at 21 h (P<0.05). Lipid peroxidation, measured by d-ROMs, remained unchanged throughout the study. Thus, intermittent exercise in well-trained female athletes induces a transient increase in GSSG and a decrease in the GSH:GSSG ratio, which is effectively balanced by the recruitment of both endogenous and dietary antioxidants, resulting in the absence of lipid peroxidation measured by d-ROMs.
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| 5. |
- Gjelstad, I. M. F., et al.
(författare)
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Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance
- 2012
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Ingår i: Archives of Physiology and Biochemistry. - : Informa UK Limited. - 1381-3455 .- 1744-4160. ; 118:1, s. 22-30
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Tidskriftsartikel (refereegranskat)abstract
- The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.
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| 6. |
- Paulsen, G., et al.
(författare)
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Strength training elevates HSP27, HSP70 and alpha B-crystallin levels in musculi vastus lateralis and trapezius
- 2012
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Ingår i: European Journal of Applied Physiology. - Heidelberg, Germany : Springer. - 1439-6319 .- 1439-6327. ; 112:5, s. 1773-1782
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Tidskriftsartikel (refereegranskat)abstract
- A single bout of high-force exercise has been shown to increase the muscle levels of heat shock proteins (HSPs). Here, changes in the levels of HSPs after 2 and 11 weeks of strength training with either one or three sets per exercise were examined. Fifteen young men (27 +/- A 6 years, 182 +/- A 8 cm and 82 +/- A 13 kg) were randomized to train either one set in lower-body exercises and three sets in upper-body exercises (1L-3UB), or three sets in lower-body exercises and one set in upper-body exercises (3L-1UB). Biopsies from vastus lateralis and trapezius were obtained before, during (2 weeks) and after 11 weeks of strength training (3 bouts per week). The biopsies were analysed for HSP27 (cytosolic and cytoskeletal fractions) and HSP70 and alpha B-crystallin (cytosolic fraction). No evidence for an effect of training volume (1 vs. 3 sets) on the HSP response was found. For all subjects combined, HSP27 [186 +/- A 69% (mean +/- A SD)], HSP70 (146 +/- A 51%) and alpha B-crystallin (184 +/- A 82%) increased in the cytosolic fraction of vastus lateralis after 11 weeks of training. In the trapezius, the only observed increase was for HSP27 in the cytosolic fraction after 2 weeks of training (149 +/- A 59%). However, the trapezius contained somewhat higher levels of HSP70 and alpha B-crystallin than vastus lateralis at baseline. The HSP27 levels in the cytoskeletal compartment did not increase significantly in either muscle. In conclusion, strength training resulted-independent of training volume-in elevated levels of HSP27, HSP70 and alpha B-crystallin in the cytosolic compartment of the vastus lateralis. In the trapezius, only the cytosolic HSP27 levels were increased with training.
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