| 1. |
- Anderson, Beverley H., et al.
(författare)
-
Mutations in CTC1, encoding conserved telomere maintenance component 1, cause Coats plus
- 2012
-
Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 44:3, s. 338-342
-
Tidskriftsartikel (refereegranskat)abstract
- Coats plus is a highly pleiotropic disorder particularly affecting the eye, brain, bone and gastrointestinal tract. Here, we show that Coats plus results from mutations in CTC1, encoding conserved telomere maintenance component 1, a member of the mammalian homolog of the yeast heterotrimeric CST telomeric capping complex. Consistent with the observation of shortened telomeres in an Arabidopsis CTC1 mutant and the phenotypic overlap of Coats plus with the telomeric maintenance disorders comprising dyskeratosis congenita, we observed shortened telomeres in three individuals with Coats plus and an increase in spontaneous gamma H2AX-positive cells in cell lines derived from two affected individuals. CTC1 is also a subunit of the alpha-accessory factor (AAF) complex, stimulating the activity of DNA polymerase-alpha primase, the only enzyme known to initiate DNA replication in eukaryotic cells. Thus, CTC1 may have a function in DNA metabolism that is necessary for but not specific to telomeric integrity.
|
|
| 2. |
- Quaresimin, Marino, et al.
(författare)
-
Damage evolution under cyclic multiaxial stress state : A comparative analysis between glass/epoxy laminates and tubes
- 2014
-
Ingår i: Composites Part B. - : Elsevier BV. - 1359-8368 .- 1879-1069. ; 61, s. 282-290
-
Tidskriftsartikel (refereegranskat)abstract
- In this work an experimental investigation on damage initiation and evolution in laminates under cyclic loading is presented. The stacking sequence [0/θ2/0/−θ2]s has been adopted in order to investigate the influence of the local multiaxial stress state in the off-axis plies and the possible effect of different thickness between the thin (2-plies) and the thick (4-plies) layers. Results are presented in terms of S–N curves for the initiation of the first cracks, crack density evolution, stiffness degradation and Paris-like curves for the crack propagation phase. The values of the off-axis angle θ has been chosen in order to obtain local multiaxial stress states in the off-axis plies similar to those in previous studies for biaxially loaded tubes. Results concerning damage initiation and growth for these two specimen configurations are shown to be consistent for similar local multiaxial stress states
|
|
| 3. |
- Quaresimin, Marino, et al.
(författare)
-
Reprint of: Damage evolution under cyclic multiaxial stress state : a comparative analysis between glass/epoxy laminates and tubes
- 2014
-
Ingår i: Composites Part B. - : Elsevier BV. - 1359-8368 .- 1879-1069. ; 65, s. 2-10
-
Tidskriftsartikel (refereegranskat)abstract
- In this work an experimental investigation on damage initiation and evolution in laminates under cyclic loading is presented. The stacking sequence [0/θ2/0/-θ2]s has been adopted in order to investigate the influence of the local multiaxial stress state in the off-axis plies and the possible effect of different thickness between the thin (2-plies) and the thick (4-plies) layers. Results are presented in terms of S–N curves for the initiation of the first cracks, crack density evolution, stiffness degradation and Paris-like curves for the crack propagation phase. The values of the off-axis angle θ has been chosen in order to obtain local multiaxial stress states in the off-axis plies similar to those in previous studies for biaxially loaded tubes. Results concerning damage initiation and growth for these two specimen configurations are shown to be consistent for similar local multiaxial stress states
|
|
| 4. |
- Gerlach, Samantha L., et al.
(författare)
-
Anticancer and Chemosensitizing Abilities of Cycloviolacin O2 from Viola odorata and Psyle Cyclotides from Psychotria leptothyrsa
- 2010
-
Ingår i: Biopolymers. - : Wiley. - 0006-3525 .- 1097-0282. ; 94:5, s. 617-625
-
Tidskriftsartikel (refereegranskat)abstract
- Cycloviolacin O2 (CyO2), a cyclotide from Viola odorata (Violaceae) has antitumor effects and causes cell death by membrane permeabilization. In the breast cancer line, MCF-7 and its drug resistant subline MCF-7/ADR, the cytotoxic effects of CyO2 (0.2-10 mu M) were monitored in the presence and absence of doxorubicin (0.1-5 mu M) using cell proliferation assays to establish its chemosensitizing abilities. SYTOX Green assays were performed to verify membrane permeabilization and showed cellular disruption correlates with cyclo tide chemosensitization. Fluorescence microscopy studies demonstrated increased cellular internalization of doxorubicin in drug resistant cells when coexposed to CyO2. Interestingly, CyO2 did not produce significant membrane disruption in primary human brain endothelial cells, which suggested cyclo tide specificity toward induced pore formation in highly proliferating tumor cells. Furthermore, three novel cyclotides (psyle A, C and E) from Psychotria leptothyrsa (Rubiaceae) were also monitored for cytotoxic activity. The cyclotides displayed potent cytotoxicity (IC50 = 0.64->10 mu M), and coexposure to cyclotides significantly enhanced doxorubicin induced toxicity (IC50 = 0.39-0.76 mu M). This study documents several cyclotides with robust cytotoxicity that may be promising chemosensitizing agents against drug resistant breast cancer.
|
|
| 5. |
|
|
