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- Kaur-Kahlon, G., et al.
(författare)
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Response of a coastal tropical pelagic microbial community to changed salinity and temperature
- 2016
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Ingår i: Aquatic Microbial Ecology. - 0948-3055 .- 1616-1564. ; 77:1, s. 37-50
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Tidskriftsartikel (refereegranskat)abstract
- Studies on the responses of tropical microbial communities to changing hydrographic conditions are presently poorly represented. We present here the results from a mesocosm experiment conducted in southwest (SW) coastal India to investigate how changes in temperature and salinity may affect a coastal tropic microbial community. The onset of algal and bacterial blooms, the maximum production and biomass, and the interrelation between phytoplankton and bacteria were studied in replicated mesocosms. The treatments were set up featuring ambient conditions (28 °C, 35 PSU), hyposalinity (31 PSU), warming (31 °C) and a double manipulated treatment with warming and hyposalinity (31 °C, 31 PSU). The hyposaline treatment had the most considerable influence manifested as significantly lower primary production, and the most dissimilar microphytoplankton species community. The increased temperature acted as a catalyst in the double manipulated treatment and higher primary production was maintained. We investigated the dynamics of the microbial community with a structural equation model approach, and found a significant interrelation between phytoplankton biomass and bacterial abundance. Using this methodology, it became evident that temperature and salinity changes, individually and together, mediate direct and indirect effects that influence different compartments of the microbial loop. In the face of climate change, we suggest that in relatively nutrient replete tropical coastal zones, salinity and temperature changes will affect nutrient assimilation with subsequent significant effects on the quantity of microbial biomass and production.
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| 3. |
- Axelsson, Carolina, 1967-, et al.
(författare)
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Optimization of several parameters in order to reduce time in antibiotic susceptibility testing in a clinical laboratory
- 2017
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Background - When sepsis or bacteraemia is suspected, patient blood samples are cultivated in blood culture bottles and then further incubated for identification of the organism and antimicrobial susceptibility testing. These methods are slow, identifying causative pathogens in a couple of hours, and antibiotic susceptibility results within 18-36 hours.Here we present optimization of several parameters in order to evaluate if the MBT ASTRA™ method can be a rapid tool, used for routine antibiotics susceptibility testing, in a clinical laboratory.Methods – MALDI-TOF MS measurements were performed with a Microflex LT/SH bench-top mass spectrometer (Bruker) with standard settings. The resulting spectra were uploaded in the MBT-ASTRA™ software, which normalizes the peaks and determines the AUC and RG values for each setup.Results - The bacterial preparation steps generated a new protocol, which reduced time with 30-60 minutes.The antibiotics susceptibility test was optimized for 90 minutes incubation time. 200 µl McFarland 0.5 bacterial suspension in broth were incubated in broth at 37°C, with and without 32 µg/ml Cefotaxime, 16 µg/ml Meropenem and 4 µg/ml Ciprofloxacin.The suspensions were transferred to 0.45 µm pore size filter membraned 96 well plate. They were centrifuged; washed; fixated and eluted; put on a MALDI-target, and covered by matrix solution. All could be automated with robot, which reduced time with 60 minutes.Conclusion – Rapid susceptibility testing becomes more requested with the increase of resistance bacteria causing infections. Our study can be a valuable tool for clinical laboratories striving for reduction in time handling of antibiotic susceptibility testing.
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Man and Biosphere Health : en komplett akademisk miljö
- 2018
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- Inom forskningsmiljön Man and Biospere Health, förkortat MABH (hkr.se/mabh), bedrivs forskning inom biologi, kemi, biomedicin och psykologi, men också mycket tvärvetenskaplig forskning, med fokus kring människans påverkan på ekosystem och dess effekter på biologiskt liv, mångfald och hälsa. Forskare inom MABH är också involverade i åtgärdsprogram och utveckling av nya tekniker och metoder för att kunna undersöka de negativa effekterna av människans aktiviteter. Forskningsmiljön MABH bildades 2009 med syfte att samla högskolan forskning inom bland annat ekologi, miljöteknik, kemi och biomedicin under ett paraply. Ambitionen var att utveckla tvärvetenskapliga projekt såväl inom som utanför gruppen.
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| 5. |
- Rehnstam-Holm, Ann-Sofi, 1959-, et al.
(författare)
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ESBL resistance patterns among environmental and clinical bacterial isolates
- 2017
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- ObjectivesWe have studied the phenotypic and genetic pattern among Extended Spectrum Beta-lactamase (ESBL) producing bacteria in the aquatic environment, and have compared the result with clinical samples from the same area.MethodsWater samples have been collected at three different sites in the Helge river, Kristianstad community, Sweden. The first station is located before the outlet from the municipal sewage plant, the second just after the outlet and the third close to the Baltic Sea. Cultured bacterial isolates from the water and clinical isolates were analysed for phenotypic expression of ESBL related genes using the MAST-test, and genetically by PCR analyses of a set of ESBL genes, i.e. blaCTX-M, blaSHV, blaTEM, and blaOXA ResultsBoth clinical and environmental ESBL isolates were dominated by Escherichia coli and Klebsiella pneumoniae. Highest abundance of environmental ESBL isolates were obtained from the station close to the sewage outlet, the lowest close to the Baltic Sea. The gene cluster blaCTX-M was the most common among all isolates (65%), followed by blaTEM (30%). The blaOXA and blaSHV genes were more common in clinical isolates.ConclusionA majority of the ESBL bacteria were mediated by chromosomal genes, dominated by blaCTX-M. However, blaOXA .and blaSHV were more common in clinical isolates. Further genetic analyses will be performed on more isolates, and on total bacterial community DNA.
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| 6. |
- Rehnstam-Holm, Ann-Sofi, 1959-, et al.
(författare)
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Forskningsanknuten grundutbildning : utbildningsanknuten forskning?
- 2018
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Ingår i: Man and Biosphere Health. - Kristianstad : Högskolan Kristianstad. - 9789187973352 ; , s. 8-15
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Begreppet forskningsanknuten utbildning kan definieras på ett flertal sätt. Idealt ska studenterna inom sin utbildning stöta på forskning genom att få kännedom om aktuell forskning och vara praktiskt involverade i forskningsprojekt. För att detta ska uppnås bör majoriteten av lärarna vara forskningsaktiva inom för utbildningen relevanta områden och att ett vetenskapligt förhållningssätt till kunskap förmedlar på bästa pedagogiska sätt också detta förankrat i forskning. I den här artikeln ger vi exempel på hur studenter har involverats i våra forskningsprojekt både på högskolan, på universitetssjukhuset i Lund och vid internationella universitet och forskningsstationer.
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