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Histo-blood group a...
Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection
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- Rimkute, Inga (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Institute of Biomedicine, Department of Microbiology and Immunology,Department of Laboratory Medicine,Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Thorsteinsson, Konrad, 1991- (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Marta Bally
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- Henricsson, Marcus, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory,Wallenberg Laboratory, Department of Molecular and Clinical Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Tenge, V. R. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA
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- Yu, X. M. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA
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- Lin, S. C. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA
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- Haga, K. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA
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- Atmar, R. L. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA; Department of Medicine, Baylor College of Medicine, Houston, Texas, USA
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- Lycke, Nils Y, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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- Nilsson, Jonas, 1970 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Laboratory of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden
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- Estes, M. K. (författare)
- Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA; Department of Medicine, Baylor College of Medicine, Houston, Texas, USA
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- Bally, Marta (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Marta Bally
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- Larson, Göran, 1953 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Laboratory of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden
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(creator_code:org_t)
- Elsevier, 2020
- 2020
- Engelska.
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Ingår i: Journal of Biological Chemistry. - : Elsevier. - 0021-9258 .- 1083-351X. ; 295:47, s. 15974-15987
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The molecular mechanisms behind infection and propagation of human restricted pathogens such as human norovirus (HuNoV) have defied interrogation because they were previously unculturable. However, human intestinal enteroids (HIEs) have emerged to offer unique ex vivo models for targeted studies of intestinal biology, including inflammatory and infectious diseases. Carbohydrate-dependent histo-blood group antigens (HBGAs) are known to be critical for clinical infection. To explore whether HBGAs of glycosphingolipids contribute to HuNoV infection, we obtained HIE cultures established from stem cells isolated from jejunal biopsies of six individuals with different ABO, Lewis, and secretor genotypes. We analyzed their glycerolipid and sphingolipid compositions and quantified interaction kinetics and the affinity of HuNoV virus-like particles (VLPs) to lipid vesicles produced from the individual HIE-lipid extracts. All HIEs had a similar lipid and glycerolipid composition. Sphingolipids included HBGA-related type 1 chain glycosphingolipids (GSLs), with HBGA epitopes corresponding to the geno- and phenotypes of the different HIEs. As revealed by single-particle interaction studies of Sydney GII.4 VLPs with glycosphingolipid-containing HIE membranes, both binding kinetics and affinities explain the patterns of susceptibility toward GII.4 infection for individual HIEs. This is the first time norovirus VLPs have been shown to interact specifically with secretor gene-dependent GSLs embedded in lipid membranes of HIEs that propagate GII.4 HuNoV ex vivo, highlighting the potential of HIEs for advanced future studies of intestinal glycobiology and host-pathogen interactions.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- glycerophospholipid
- glycosphingolipid
- sphingomyelin
- ceramide
- host-pathogen interaction
- intestinal epithelium
- human norovirus
- viral
- replication
- sphingolipid
- glycosylation
- glycolipid structure
- intestine
- fucosyltransferase
- plus-stranded RNA virus
- virus entry
- cell-surface antigens
- virus-like particles
- epithelial-cells
- molecular
- characterization
- membrane-properties
- fetal tissue
- stem-cells
- mini-guts
- meconium
- expression
- Biochemistry & Molecular Biology
- ceramide
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Rimkute, Inga
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Thorsteinsson, K ...
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Henricsson, Marc ...
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Tenge, V. R.
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Yu, X. M.
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Lin, S. C.
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Haga, K.
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Atmar, R. L.
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Lycke, Nils Y, 1 ...
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Nilsson, Jonas, ...
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Estes, M. K.
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Bally, Marta
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Larson, Göran, 1 ...
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Göteborgs universitet
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