| 1. |
- Almeida, Teresa, et al.
(författare)
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Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosis.
- 2008
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Ingår i: Molecular biology of the cell. - 1939-4586. ; 19:3, s. 865-76
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Tidskriftsartikel (refereegranskat)abstract
- The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1Delta mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1Delta mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.
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| 2. |
- Rodrigues, Ana, et al.
(författare)
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Generating functions for Hopf bifurcation with Sn-symmetry
- 2009
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Ingår i: Discrete and Continuous Dynamical Systems. - : American Institute of Mathematical Sciences (AIMS). - 1078-0947 .- 1553-5231. ; 25:3, s. 823-842
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Tidskriftsartikel (refereegranskat)abstract
- Hopf bifurcation in the presence of the symmetric group (acting naturally by permutation of coordinates) is a problem with relevance to coupled oscillatory systems. To study this bifurcation it is important to construct the Taylor expansion of the equivariant vector field in normal form. We derive generating functions for the numbers of linearly independent invariants and equivariants of any degree, and obtain recurrence relations for these functions. This enables us to determine the number of invariants and equivariants for all , and show that this number is independent of for sufficiently large . We also explicitly construct the equivariants of degree three and degree five, which are valid for arbitrary .
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| 3. |
- Rodrigues, Ana, et al.
(författare)
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Hopf bifurcation with SN-symmetry
- 2009
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Ingår i: Nonlinearity. - : IOP Publishing. - 0951-7715 .- 1361-6544. ; 22:3, s. 627-666
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Tidskriftsartikel (refereegranskat)abstract
- We study Hopf bifurcation with SN-symmetry for the standard absolutely irreducible action of SN obtained from the action of SN by permutation of N coordinates. Stewart (1996 Symmetry methods in collisionless many-body problems, J. Nonlinear Sci. 6 543–63) obtains a classification theorem for the C-axial subgroups of SN × S1. We use this classification to prove the existence of branches of periodic solutions with C-axial symmetry in systems of ordinary differential equations with SN-symmetry posed on a direct sum of two such SN-absolutely irreducible representations, as a result of a Hopf bifurcation occurring as a real parameter is varied. We determine the (generic) conditions on the coefficients of the fifth order SN × S1-equivariant vector field that describe the stability and criticality of those solution branches. We finish this paper with an application to the cases N = 4 and N = 5.
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