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- Siegbahn, Agneta, et al.
(författare)
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Production of chemokinetic inhibitory factor (CIF) by normal blood and spleen B lymphocytes
- 1986
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Ingår i: Leukemia Research. - 0145-2126 .- 1873-5835. ; 10:2, s. 179-186
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We have recently reported the partial purification and characterization of of a new lymphokine, the heat-labile chemokinetic inhibitory factor (CIF) which inhibits neutrophil movement. We have also shown that this lymphokine is produced and secreted by cultured B-chronic lymphocytic leukaemia (CLL) cells in vitro. The present study shows that highly purified resting normal B lymphocytes from blood and spleen have the capacity to produce CIF spontaneously. After activation with anti-IgM or EBV-infection the lymphocytes produced a number of other factors, heat-stable chemokinetic inhibitory factors and heat-labile chemokinetic enhancing factors. Supernatants from a collection of human B-cell lines representing different stages of B-cell differentiation were also investigated. None of these cell lines produced CIF. The present results show that the production of CIF is not restricted to the malignant B-CLL cell but is also produced by a subset of normal blood and spleen B cells.
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