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SM-Omics is an auto...
SM-Omics is an automated platform for high-throughput spatial multi-omics
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- Vickovic, Sanja (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Broad Institute of MIT and Harvard, USA; Massachusetts Institute of Technology, USA; New York Genome Center, USA,Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;MIT, Dept Biol, Cambridge, MA 02139 USA.;New York Genome Ctr, New York, NY 10013 USA.;Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Solna, Sweden.
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- Lötstedt, Britta (författare)
- KTH,Genteknologi,Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA.
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- Klughammer, J. (författare)
- Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.
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- Mages, S. (författare)
- Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.
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- Segerstolpe, A. (författare)
- Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.
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- Rozenblatt-Rosen, O. (författare)
- Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;Genentech Inc, 1 DNA Way, San Francisco, CA 94080 USA.
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- Regev, A. (författare)
- Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA.;MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA.;MIT, Koch Inst Integrat Canc Res, Dept Biol, Cambridge, MA 02139 USA.;Genentech Inc, 1 DNA Way, San Francisco, CA 94080 USA.
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(creator_code:org_t)
- 2022-02-10
- 2022
- Engelska.
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The spatial organization of cells and molecules plays a key role in tissue function in homeostasis and disease. Spatial transcriptomics has recently emerged as a key technique to capture and positionally barcode RNAs directly in tissues. Here, we advance the application of spatial transcriptomics at scale, by presenting Spatial Multi-Omics (SM-Omics) as a fully automated, high-throughput all-sequencing based platform for combined and spatially resolved transcriptomics and antibody-based protein measurements. SM-Omics uses DNA-barcoded antibodies, immunofluorescence or a combination thereof, to scale and combine spatial transcriptomics and spatial antibody-based multiplex protein detection. SM-Omics allows processing of up to 64 in situ spatial reactions or up to 96 sequencing-ready libraries, of high complexity, in a similar to 2 days process. We demonstrate SM-Omics in the mouse brain, spleen and colorectal cancer model, showing its broad utility as a high-throughput platform for spatial multi-omics.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
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- ref (ämneskategori)
- art (ämneskategori)
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