A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae.

• While light is a crucial energy source in photosynthetic organisms, if its intensity exceeds their photosynthetic capacity it may cause light-induced damage. A dominant photoprotective mechanism in plants and algae is the qE (quenching of energy), the major component of nonphotochemical quenching (NPQ). qE is a process that dissipates absorbed excitation energy as heat, ensuring cell survival even under adverse conditions. The present protocol gathers together a set of experimental approaches (in vivo chlorophyll fluorescence, western blotting, growth and cellular chlorophyll content at very strong light) that collectively allow for the characterization of the qE capacity of the model green algae Chlamydomonas reinhardtii.

Ämnesord

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

Nyckelord

High light stress

In vivo chlorophyll fluorescence

Nonphotochemical quenching (NPQ)

Quenching of energy (qE)

Western blotting

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)