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Sökning: WFRF:(Russell J. J.) > (1990-1994) > The Crl protein act...

The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HB101.

Arnqvist, Anna (författare)
Olsén, A (författare)
Pfeifer, J (författare)
visa fler...
Russell, D G (författare)
Normark, S (författare)
visa färre...
1992
1992
Engelska.
Ingår i: Molecular Microbiology. - 0950-382X .- 1365-2958. ; 6:17, s. 2443-52
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Curli are thin, coiled, temperature-regulated fibres on fibronectin-binding Escherichia coli. The subunit protein of curli was highly homologous at its amino terminus to SEF-17, the subunit protein of thin, aggregative fimbriae of Salmonella enteritidis 27655 strain 3b, suggesting that these fibres form a novel class of surface organelles on enterobacteria. E. coli HB101 is non-curliated and unable to bind soluble, iodinated fibronectin. The phenotypically cryptic curlin subunit gene, csgA, in HB101 is transcriptionally activated by expressing the cytoplasmic Crl on a multicopy plasmid. Transcriptional activation of csgA by Crl was observed after growth at 26 degrees C but not at 37 degrees C, even though crl transcription was not thermoregulated. A deletion of the 39 carboxy-terminal residues abolished Crl activity, whereas a deletion of 10 residues at the C-terminus did not, implying that a region between residue 93 and 122 in the 132-amino-acid-residue large Crl protein is required for activating curli expression in E. coli HB101. crl is a normal housekeeping gene in E. coli and it is suggested that its gene product may either be a DNA-binding protein affecting chromatin structure as has been suggested for histone-like protein H1 or interact with specific regulatory protein(s) controlling transcription of genes required for curli formation and fibronectin binding.

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Av författaren/redakt...
Arnqvist, Anna
Olsén, A
Pfeifer, J
Russell, D G
Normark, S
Artiklar i publikationen
Molecular Microb ...
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Umeå universitet

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